Diagnosis of SARS-CoV-2 Infection with LamPORE, a High-Throughput Platform Combining Loop-Mediated Isothermal Amplification and Nanopore Sequencing루프 매개 등온 증폭과 나노포어 시퀀싱을 결합한 고처리량 플랫폼인 LamPORE를 사용한 SARS-CoV-2 감염 진단Virology Published on 2021-03-292022-09-10 Journal: Journal of Clinical Microbiology [Category] SARS, 치료기술, [키워드] 95% confidence interval acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 approach clinical samples collected coronavirus Diagnosis diagnosis of SARS-CoV-2 diagnostic diagnostic sensitivity diagnostic specificity evaluated extracted RNA Genome Infection isothermal amplification LamPORE limit limit of detection Loop-mediated isothermal amplification Nanopore nanopore sequencing nose and throat offer Pathogens Patient per day platform produced reduction reproducibility respiratory respiratory pathogen respiratory sample reverse transcriptase reverse transcriptase PCR RNA RT-PCR SARS-CoV-2 SARS-COV-2 infection sensitivity Sequencing severe acute respiratory syndrome Coronavirus specificity specimen specimens Swab symptomatic infection symptomatic patient Symptomatic patients throat swabs UK hospitals [DOI] 10.1128/JCM.03271-20 PMC 바로가기 [Article Type] Virology
Comparison of rhesus and cynomolgus macaques as an infection model for COVID-19Article Published on 2021-02-242022-10-04 Journal: Nature Communications [Category] MERS, 유전자 메커니즘, 진단, 치료기술, [키워드] a metric alleviate Analysis animal animal model cause convalescent COVID-19 COVID-19 disease COVID-19 pandemic cynomolgus macaque cynomolgus macaques Efficacy emerging disease Endpoint Evidence form Infection Intervention Lower respiratory tract Lung histopathology majority Mild milder infection moderate Non-human non-human primates Novel coronavirus Pathogenesis patients pulmonary lesion replicate reported respiratory sample responses against rhesus SARS-CoV-2 SARS-COV-2 infection scoring system transcriptional Translational research used to evaluate Vaccine viral infection [DOI] 10.1038/s41467-021-21389-9 PMC 바로가기 [Article Type] Article
SARS-CoV-2 RNA in plasma samples of COVID-19 affected individuals: a cross-sectional proof-of-concept studyCOVID-19 영향을 받는 개인의 혈장 샘플에서 SARS-CoV-2 RNA: 횡단면 개념 증명 연구Research Article Published on 2021-02-172022-09-01 Journal: BMC Infectious Diseases [Category] MERS, 진단, [키워드] affected Blood characterized COVID-19 cross-sectional ddPCR develop Fisher exact test haematologic haematological malignancies haematological malignancy help Hematological malignancies higher risk hospital Hospitalized individual IQR laboratory data management median Molecular diagnosis mortality rate outcome Patient patients with COVID-19 plasma plasma sample predict Prognosis quantified Quantitative recent respiratory respiratory sample Respiratory samples Result SARS-CoV-2 SARS-CoV-2 RNA severe COVID-19 significant difference significant differences supplementary material Support variables Viral Viral load Viremia were used Wilcoxon test [DOI] 10.1186/s12879-021-05886-2 PMC 바로가기 [Article Type] Research Article
Molecular Evolution of Human Coronavirus 229E in Hong Kong and a Fatal COVID-19 Case Involving Coinfection with a Novel Human Coronavirus 229E Genogroup홍콩에서 인간 코로나바이러스 229E의 분자 진화와 새로운 인간 코로나바이러스 229E 유전자 그룹과의 동시 감염을 포함하는 치명적인 COVID-19 사례Case Reports Published on 2021-02-102022-09-10 Journal: mSphere [Category] MERS, 진단, 치료기술, [키워드] 229E addition airway Analysis camelid circulating CLOCK Clustering Coinfection COVID-19 COVID-19 pneumonia described diagnose disease domain Evolution Genetic genetic diversity genetic drift Genome sequencing Genotype HCoV HCoV-229 HCoV-229E Hong Kong human coronavirus human coronavirus 229E human coronaviruses identity infected patients Involving N gene nasopharyngeal sample novel nucleotide path patients Pneumonia positive Positive selection RdRP Receptor-binding domain related viruses required respiratory respiratory sample retrieved SARS-CoV-2 SARS-CoV-2 strain Sequence analysis Sequencing Spike protein strain Strains tested the patient the SARS-CoV-2 the spike protein the United State The United States Transmission Travel type strain United States Viral viral evolution virus viruses Wuhan Wuhan-Hu-1 [DOI] 10.1128/mSphere.00819-20 PMC 바로가기 [Article Type] Case Reports
Characteristics of Viral Shedding in Respiratory Samples and Specific Antibodies Production in 564 COVID-19 Patients564명의 COVID-19 환자에서 호흡기 샘플 및 특정 항체 생산의 바이러스 배출 특성Article Published on 2021-02-012022-09-12 Journal: Current medical science [Category] MERS, 진단, [키워드] accounted age antibody antibody production antibody tests branch changes in city collected Concentration concentrations correlated correlation COVID-19 COVID-19 patient COVID-19 patients Day discharge discharged disease severity Gender had no IgG and IgM IgM Inpatient median nasopharyngeal swabs New nucleic acid Patient patients positive positive rate Production proportion quarantine reduce Reporting respiratory respiratory sample Sample SARS-CoV-2 shedding significantly Specific specific antibodies specific antibody sputum suggested symptom onset the patient throat swabs Tongji Hospital viral shedding were recorded [DOI] 10.1007/s11596-021-2316-3 PMC 바로가기 [Article Type] Article
Frequency of respiratory pathogens and SARS‐CoV ‐2 in canine and feline samples submitted for respiratory testing in early 2020Paper Published on 2021-01-312022-10-30 Journal: The Journal of Small Animal Practice [Category] COVID-19, SARS, [키워드] 2019 novel coronavirus Asia Bordetella calicivirus canine caused clinical Europe evaluated Frequency Laboratory material Mycoplasma North America objective pandemic pathogen Patient PCR PCR positive Respiratory disease respiratory pathogen respiratory sample Result Sample SARS‐CoV‐2 SARS‐CoV‐2 infection Significance submitted subset tested [DOI] 10.1111/jsap.13300 PMC 바로가기 [Article Type] Paper
Summary of the Available Molecular Methods for Detection of SARS-CoV-2 during the Ongoing Pandemic진행중인 유행성 전염병 동안 SARS-CoV-2의 검출에 사용 가능한 분자 방법의 요약Review Published on 2021-01-282022-09-10 Journal: International Journal of Molecular Sciences [Category] MERS, 변종, 진단, 치료기술, 치료제, [키워드] 501Y.V2 501Y.V2 variant affected B.1.1.7 variant caused Containment containment strategies Coronaviridae COVID-19 COVID-19 pandemic described detection Diagnosis diagnostic diagnostic protocol Evolution excess genetic variability genomic gold gold standard in silico analysis Laboratory laboratory diagnosis molecular molecular methods morbidity morbidity and mortality mortality rates Mutation no effect pandemic probes protocol protocols Region reliability respiratory respiratory sample Respiratory samples SARS-CoV-2 SARS-CoV-2 genome sensitivity situation South Africa target UK variant variants Viral viral genome virus virus variant [DOI] 10.3390/ijms22031298 PMC 바로가기 [Article Type] Review
MERS-CoV: epidemiology, molecular dynamics, therapeutics, and future challengesReview Published on 2021-01-182022-10-28 Journal: Annals of Clinical Microbiology and Antimicrobials [Category] COVID-19, MERS, [키워드] affected Betacoronavirus challenges changes in Cluster Community coronavirus coronavirus 2 Cross-species transmission effective Epidemic Epidemiology event Factor Fatality rate genetic variability Genome highest highly pathogenic immunological inherent membrane MERS MERS-CoV Middle East molecular molecular dynamics Nosocomial infection nucleocapsid nucleotide occur open reading frames outbreak pandemic pathology Phylogeny predicted progression Protein Region reported Research respiratory respiratory sample SARS-CoV-2 Saudi Arabia Seven spike Spike protein structural proteins subsequent therapeutic Therapeutics Transmission viral infection zoonotic zoonotic transmission [DOI] 10.1186/s12941-020-00414-7 PMC 바로가기 [Article Type] Review
Intermittent viral shedding in respiratory samples of patients with SARS-CoV-2: observational analysis with infection control implicationsSARS-CoV-2 환자의 호흡기 샘플에서 간헐적인 바이러스 배출: 감염 통제 의미를 가진 관찰 분석Observational Study Published on 2021-01-012022-09-12 Journal: The Journal of hospital infection [Category] MERS, SARS, 임상, 진단, [키워드] Abstract Analysis Asymptomatic clinical clinical presentation COVID-19 Hospitalization implication infection control Intermittent Patient respiratory sample SARS-CoV-2 viral shedding [DOI] 10.1016/j.jhin.2020.09.011 PMC 바로가기 [Article Type] Observational Study
Viral RNA load in plasma is associated with critical illness and a dysregulated host response in COVID-19혈장의 바이러스 RNA 부하는 COVID-19의 심각한 질병 및 조절되지 않는 숙주 반응과 관련이 있습니다Research Published on 2020-12-142022-09-11 Journal: Critical Care [Category] MERS, 바이오마커, 진단, 치료제, [키워드] Activation Analysis angiopoietin association Biomarker Biomarkers Blood CCL2 characterized chemokine chemokines Coagulation correlated correlation coefficients correlations Course COVID-19 Critical Critical illness critical patient critical patients Critically ill Critically ill patient critically ill patients CRP CXCL10 cytokine D-dimer digital PCR disease dysregulated dysregulated response dysregulation Endothelial dysfunction evaluated ferritin Frequency GM-CSF GPT highest Hospitalized host response host responses ICAM-1 ICU ICU Patients IL-10 IL-15 IL-6 INR LDH lymphopenia monocytopenia Multivariate analysis multivariate logistic regression myeloperoxidase N1 and N2 neutrophil neutrophil response Neutrophils NK cell NK cells non-critically ill patients non-survivor Non-survivors Outpatient outpatients Pathogenesis Patient patients with COVID-19 PD-L1 plasma quantification recruited Region respiratory respiratory sample Respiratory samples Result RNA RNAemia SARS-CoV-2 SARS-CoV-2 RNA Sepsis severe COVID-19 severity Spearman Stool systemic inflammatory response the SARS-CoV-2 tissue damage Urine VCAM-1 Viral viral replication Viral RNA viral RNA detection viral RNA load virus ward was performed [DOI] 10.1186/s13054-020-03398-0 PMC 바로가기 [Article Type] Research