Quantification of antibody avidities and accurate detection of SARS-CoV-2 antibodies in serum and saliva on plasmonic substrates Research Published on 2020-10-292022-10-05 Journal: Nature biomedical engineering [Category] SARS, 진단, 치료기술, [키워드] 229E acute respiratory syndrome amplification anamnestic responses antibody Antibody avidity Antigen assays collected common-cold coronavirus coronavirus disease Coronaviruses COVID-19 HKU1 human serum Immunoglobulin NL63 OC43 pandemic positive Receptor binding domain Saliva SARS-CoV-1 SARS-CoV-2 SARS-CoV-2 antibody serum serum sample substrate substrates symptom onset tested the S1 subunit the spike protein virus viruses [DOI] 10.1038/s41551-020-00642-4 [Article Type] Research
Structural basis for potent neutralization of SARS-CoV-2 and role of antibody affinity maturationArticle Published on 2020-10-272022-10-04 Journal: Nature Communications [Category] MERS, SARS, 변종, [키워드] antibody affinity Betacoronavirus bind binding binding affinity competing complex COVID-19 pandemic COVID19 determine effective epitope human ACE2 receptor induce infected with SARS-CoV-2 maturation mechanism monoclonal antibody motif Mutation neutralization Neutralizing neutralizing antibody overlap Patient Receptor binding domain reduction in responsible reveal SARS-CoV-2 the S1 subunit Treatment Vaccine viral infection virus X-ray X-ray crystallography [DOI] 10.1038/s41467-020-19231-9 PMC 바로가기 [Article Type] Article
Subunit Nanovaccine with Potent Cellular and Mucosal Immunity for COVID-19COVID-19에 대한 강력한 세포 및 점막 면역을 가진 서브유닛 나노백신Article Published on 2020-09-212022-09-12 Journal: ACS applied bio materials [Category] MERS, SARS, 진단, [키워드] activating acute respiratory syndrome adjuvants alum adjuvant cationic CD4 CD8 cells cellular cellular immunity coronavirus COVID-19 CpG elicit humoral humoral immune response Humoral immunity IgA antibody Immunity infecting inhibit SARS-CoV-2 Liposome mice nanoadjuvant nanovaccine pandemic potent receptor SARS-CoV-2 sera significantly strong T-cell the S1 subunit Vero cells virus [DOI] 10.1021/acsabm.0c00668 PMC 바로가기 [Article Type] Article
Impact of glycan cloud on the B-cell epitope prediction of SARS-CoV-2 Spike proteinArticle Published on 2020-09-042022-10-04 Journal: NPJ Vaccines [Category] 진단, [키워드] Alter Analysis antibody antibody-based therapy B-cell epitope China diagnostics epitope flexible glycosylation pattern heterogeneous homogeneous identify Impact inaccessible N-glycosylation outbreak pandemic proportions Protein Receptor binding domain resulting S-protein S2 subunit SARS-CoV-2 spike spread to target the S1 subunit the Spike trimeric Vaccine Vaccine development Vaccines were used [DOI] 10.1038/s41541-020-00237-9 PMC 바로가기 [Article Type] Article
Four SARS-CoV-2 Genome Sequences from Late April in Stockholm, Sweden, Reveal a Rare Mutation in the Spike ProteinGenome Sequences Published on 2020-08-272022-10-29 Journal: Microbiology Resource Announcements [Category] COVID-19, [키워드] acute respiratory syndrome arginine coronavirus genome sequence histidine residue SARS-CoV-2 sequence the S1 subunit the SARS-CoV-2 genome the Spike the spike protein variant [DOI] 10.1128/MRA.00934-20 PMC 바로가기 [Article Type] Genome Sequences
Broad and Differential Animal Angiotensin-Converting Enzyme 2 Receptor Usage by SARS-CoV-2Virus-Cell Interactions Published on 2020-07-132022-10-30 Journal: Journal of Virology [Category] COVID-19, MERS, [키워드] ACE2 ACE2 ortholog activities amplifying animal animal ACE2 Animal host animal hosts animal models bat caused cleavage coronavirus Coronaviruses COVID-19 pandemic CoVs determine effort Entry examined exhibited furin furin cleavage furin cleavage site global public health glycoprotein highest host cell host cells host range hosts human ACE2 human Angiotensin-converting enzyme human-to-animal transmission implication infect cells Infection Interaction Lentiviral particle lowest molecular basis Mutant SARS-CoV-2 pangolins Pathogenesis polybasic pseudotyped Pseudotyped virus receptor Receptor binding reservoirs S protein SARS-CoV-2 SARS-CoV-2 S protein Spike protein Support supported the S protein the S1 subunit tissue tropism Transmission Usage wild-type zoonotic transmission [DOI] 10.1128/JVI.00940-20 PMC 바로가기 [Article Type] Virus-Cell Interactions
Cryo-EM structures of HKU2 and SADS-CoV spike glycoproteins provide insights into coronavirus evolutionArticle Published on 2020-06-172022-10-29 Journal: Nature Communications [Category] COVID-19, [키워드] bats C-terminal domain China coronavirus coronavirus spike proteins Coronaviruses Cross-species transmission cryo-EM structure Cryoelectron microscopy diarrhea domain Evolution fusion peptide Genome glycoprotein Human identity infecting S2 subunit spike spike glycoprotein spikes Structure the S1 subunit the spike protein trimer unique viral evolution Virus structures α-coronavirus β-coronavirus [DOI] 10.1038/s41467-020-16876-4 PMC 바로가기 [Article Type] Article
Role of changes in SARS-CoV-2 spike protein in the interaction with the human ACE2 receptor: An in silico analysisOriginal Article Published on 2020-03-182022-10-31 Journal: EXCLI Journal [Category] COVID-19, [키워드] ACE2 affected Analysis binding energy caused change changes in coronavirus determine Epidemic Epidemics event higher affinity host receptor human ACE2 human ACE2 receptor humans in silico in silico Approach Interaction Ligand magnitude outbreak promote receptor reduction in role SARS-CoV-2 SARS-CoV-2 spike protein sequence significant effect spike Spike protein the disease the receptor-binding domain the S1 subunit the SARS-CoV-2 the spike protein Transmission viral disease Viral protein viral spread was performed zoonotic [DOI] 10.17179/excli2020-1167 PMC 바로가기 [Article Type] Original Article
Cryo-EM analysis of a feline coronavirus spike protein reveals a unique structure and camouflaging glycans530 Published on 2020-01-212022-10-29 Journal: Proceedings of the National Academy of Sciences of [Category] Coronavirus, [키워드] Activation alphacoronavirus analyses Analysis cause cleavage site complex protein coronavirus spike protein CoV cryo-EM cryo-EM structure Cryoelectron microscopy demonstrated domain effective feasibility feature Feline infectious peritonitis Feline infectious peritonitis virus glycan glycosylation high-mannose highlight Host mass spectrometry Microscopy molecular mortality rate N-glycan Pathogenesis Protein protein glycosylation provided reported responsible resulting reveal S protein S proteins S2 subunit serotype Significance structures the S1 subunit Treatment unique viral entry virus [DOI] 10.1073/pnas.1908898117 PMC 바로가기 [Article Type] 530
Identification of the immunodominant neutralizing regions in the spike glycoprotein of porcine deltacoronavirusArticle Published on 2019-12-062022-11-01 Journal: Virus research [Category] Coronavirus, MERS, [키워드] Amino acid Antibody Response Antisera C-terminal domain coronavirus CTD defined E. coli effective effective therapy Epitope region expression fluorescent Host identification immunodominant in vitro in vivo Infection inhibit mice N-terminal domain neutralization Neutralization assay Neutralizing Neutralizing antibodies neutralizing antibody NTD Porcine deltacoronavirus porcine deltacoronavirus (PDCoV) produced Protein Recombinant protein Region S protein S1 subunit S2 subunit spike glycoprotein the S protein the S1 subunit Transmission Vaccine Vaccines Virus neutralization were expressed [DOI] 10.1016/j.virusres.2019.197834 PMC 바로가기 [Article Type] Article