Identification of a novel conserved HLA-A*0201-restricted epitope from the spike protein of SARS-CoVResearch article Published on 2009-12-032022-10-28 Journal: BMC Immunology [Category] Coronavirus, Fulltext, MERS, [키워드] acute respiratory syndrome analyzed assays autologous binding Candidates Cell-mediated immune responses cleavage conserved coronavirus CoV CTL dendritic cell different Donor eight epitope Ex vivo full-length S protein glycoprotein healthy help identification IFN-γ immunized immunized mice immunopathology lysed mechanism Pathogenesis PBL peptide peptides Plasmid predict processed Protein Region released Result S protein SARS-CoV SARS-CoV infection sequence target cell tested the S protein the spike protein transgenic mice virus virus clearance [DOI] 10.1186/1471-2172-10-61 PMC 바로가기 [Article Type] Research article
Identification of a novel conserved HLA-A*0201-restricted epitope from the spike protein of SARS-CoVResearch article Published on 2009-12-032022-10-27 Journal: BMC Immunology [Category] Coronavirus, [키워드] acute respiratory syndrome analyzed assays autologous binding Candidates Cell-mediated immune responses cleavage conserved coronavirus CoV CTL dendritic cell different Donor eight epitope Ex vivo full-length S protein glycoprotein healthy help identification IFN-γ immunized immunized mice immunopathology lysed mechanism Pathogenesis PBL peptide peptides Plasmid predict processed Protein Region released Result S protein SARS-CoV SARS-CoV infection sequence target cell tested the S protein the spike protein transgenic mice virus virus clearance [DOI] 10.1186/1471-2172-10-61 PMC 바로가기 [Article Type] Research article
Antibody-mediated synergy and interference in the neutralization of SARS-CoV at an epitope cluster on the spike proteinArticle Published on 2009-10-252023-06-16 Journal: Biochemical and Biophysical Research Communication [Category] MERS, [키워드] epitope monoclonal antibody neutralization SARS-CoV [DOI] 10.1016/j.bbrc.2009.10.115 PMC 바로가기 [Article Type] Article
Broadening of Neutralization Activity to Directly Block a Dominant Antibody-Driven SARS-Coronavirus Evolution PathwayResearch Article Published on 2008-11-072022-10-29 Journal: PLoS Pathogens [Category] Coronavirus, [키워드] 80R activity amino acid change Analysis Anti-viral Anti-viral therapy Author block caused CDR Combination complementarity determining region component Cytidine deaminase dominant driving effective elicited Epidemic epitope escape mutant escape mutants escape mutation Evidence Evolution found generate human convalescent sera immune immune pressure IMPROVE Infected Infectious disease information introduced investigated Mutagenesis NAb natural infection neutralization neutralization activity neutralizing antibody non-immune outbreak pathway Pathways Positive selection Protein provided response responses against responsible SARS Coronavirus SARS-CoV SARS-CoV spike protein Somatic Spike protein strain Strategy Support Therapies transfer Transmission viral variant virus viruses while zoonotic zoonotic transmission [DOI] 10.1371/journal.ppat.1000197 PMC 바로가기 [Article Type] Research Article
Prevention of Cytotoxic T Cell Escape Using a Heteroclitic Subdominant Viral T Cell DeterminantResearch Article Published on 2008-10-242022-10-29 Journal: PLoS Pathogens [Category] Coronavirus, MERS, [키워드] alteration amino acid substitution Analysis antigen-specific T cell association Author CD8+ T cell changes in clinical disease complex Complex molecule conformation Consensus Critical CTL cytotoxic determinant Disease progression Diseases elicit Enhancing epitope escape expressed functional Hepatitis histocompatibility HIV immune response immune responses immunodominant Infected Local Major memory MHC class I mice molecular basis motif mouse hepatitis mutated Mutation natural infection occur pathogen peptide performed prevention PROTECT Protective recombinant residue response significantly higher stability Subdominant T cell T cell epitope T cell response tumor immunity Vaccine development viral infection virus wildtype [DOI] 10.1371/journal.ppat.1000186 PMC 바로가기 [Article Type] Research Article
Preparation and characterization of a novel monoclonal antibody specific to severe acute respiratory syndrome-coronavirus nucleocapsid proteinArticle Published on 2006-08-302022-11-01 Journal: Virus research [Category] Coronavirus, [키워드] Antigen assays C-terminus Cell coronavirus cross-reactivity Diagnosis diagnostic early diagnosis epitope expressed FIVE found generate Host IgG immune Infection insect cell linear epitopes mAb modulation monoclonal antibody N protein N proteins N-terminus nucleocapsid nucleocapsid protein pathology phosphorylated preparation Protein reactivity related SARS-CoV SARS-CoV N selected severe Severe acute respiratory syndrome suggested viral pathogenesis virus replication [DOI] 10.1016/j.virusres.2006.07.004 PMC 바로가기 [Article Type] Article
Human Monoclonal Antibody Combination against SARS Coronavirus: Synergy and Coverage of Escape MutantsResearch Article Published on 2006-07-042022-10-29 Journal: PLoS Medicine [Category] Coronavirus, [키워드] 2003 acute respiratory syndrome Administered affected against SARS-CoV Alter Amino acid anti-SARS-CoV antibody antibody antibody concentration Antigen antigens approach arising Bacteria binding blocked Blood breadth carried caused center China close contact close person-to-person contact Combination complementary Concentration Control convalescent SARS patient coronavirus Coverage CR3014 CR3022 died different disease disease severity dose Effect effective Epidemic Epidemics epitope escape experiment feasible ferret ferrets finding generate glycoprotein Guangdong Health healthy Hong Kong Human human cell human cells human macrophage Human monoclonal antibody humans immune Immune escape immunization immunize in vitro individual effects Infected infecting Infection infectious Infectious disease information interact Interaction International killing Laboratory less limit lung damage Lung pathology mAb mAbs measure monoclonal monoclonal antibody Mutation neutralization neutralized NO organism Patient pharyngeal Prevent prevented prevention producing Prophylaxis provided quarantine recognize recognizing reduce reduction repeated replicated required Research researcher residue responsible SARS epidemic SARS-CoV SARS-CoV infection screened shown sick Spread strain suppress synergistic synergistic fashion synergy tested the disease the receptor-binding domain Travel treat variant variants viral escape viral spread virus Virus neutralization virus strain virus-neutralizing viruses website Wikipedia World Health Organization [DOI] 10.1371/journal.pmed.0030237 PMC 바로가기 [Article Type] Research Article
Generation of human antibody fragments recognizing distinct epitopes of the nucleocapsid (N) SARS-CoV protein using a phage display approachResearch Article Published on 2005-09-192022-10-28 Journal: BMC Infectious Diseases [Category] Coronavirus, [키워드] acute respiratory syndrome antibody antibody fragment Antiviral compound approach cause characterized coated with antigen described detect diagnostic test disease domain ELISA epitope Generation high mortality rate Human immunocytochemistry immunohistochemistry in vitro Infected Isolation MONITOR N protein neutralizing antibody nucleocapsid Particle Prevent Protein reagent recognize recognizing Result SARS epidemic SARS virus SARS-CoV SARS-CoV N protein scFv selected severe Spread target cells treat Vaccine Vero cells virus was used [DOI] 10.1186/1471-2334-5-73 PMC 바로가기 [Article Type] Research Article