Rapid cGMP manufacturing of COVID-19 monoclonal antibody using stable CHO cell pools안정적인 CHO 세포 풀을 사용하여 COVID-19 단일 클론 항체의 신속한 cGMP 제조Article Published on 2022-02-012022-09-11 Journal: Biotechnology and bioengineering [Category] COVID19(2023년), MERS, SARS, 임상, 진단, [키워드] accelerated activities acute respiratory syndrome acute respiratory syndrome coronavirus approach Cell cell bank Cell culture cGMP cGMP manufacturing CHO cell CHO pools clinical trial clone comparability coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 Critical demonstrated Final generate host cell material monoclonal antibodies monoclonal antibody pandemic performed Phase 1 Phase 1 clinical trial Proteins provided Rapid respiratory subsequent supply Testing timeline transposase [DOI] 10.1002/bit.27995 PMC 바로가기 [Article Type] Article
Comparative Immunogenicity of the Recombinant Receptor-Binding Domain of Protein S SARS-CoV-2 Obtained in Prokaryotic and Mammalian Expression SystemsArticle Published on 2022-01-092022-10-28 Journal: Vaccines [Category] COVID-19, [키워드] Analysis Antibody titer antigenic BALB/c mice cells CHO cell Comparative COVID-19 domain effective ELISA expressed expression humoral immune response immunized immunogenic immunogenicity maintain mammalian mice neutralization neutralizing antibody Protein protein S provide RBD Receptor-binding domain recombinant recombinant RBD protein S protein SARS-CoV-2 sera significantly subunit vaccines System target target protein the RBD Vaccine variant [DOI] 10.3390/vaccines10010096 PMC 바로가기 [Article Type] Article
Quality comparability assessment of a SARS-CoV-2-neutralizing antibody across transient, mini-pool-derived and single-clone CHO cells일시적인 미니 풀 유래 및 단일 클론 CHO 세포 전반에 걸친 SARS-CoV-2 중화 항체의 품질 동등성 평가Comparative Study Published on 2022-01-012022-09-12 Journal: mAbs [Category] SARS, 진단, [키워드] accelerate acute respiratory syndrome adopted Cell cell line Chemistry Chinese hamster CHO cell clinical trial comparability comparable conducted Control coronavirus Degradation immunological functions indicated measure mini-pool monoclonal antibody Neutralizing neutralizing antibody patients Phase 1 profiles public health crisis Quality Research SARS-CoV-2 SARS-CoV-2-neutralizing antibody Spike protein stress condition structures Support the disease the SARS-CoV-2 therapeutic transient. triggered Variation [DOI] 10.1080/19420862.2021.2005507 PMC 바로가기 [Article Type] Comparative Study
Functional Trimeric SARS-CoV-2 Envelope Protein Expressed in Stable CHO CellsBioengineering and Biotechnology Published on 2021-12-172022-10-31 Journal: Frontiers in Bioengineering and Biotechnology [Category] COVID-19, [키워드] ACE2 acute respiratory syndrome Cell cellular CHO cell coronavirus COVID-19 pandemic COVID–19 CR3022 described docking domain envelope envelope protein expressing expression Extracellular infection mechanism infection with SARS-CoV-2 integrated bioprocessing Interaction mandatory membrane-bound mutant pandemic parameter Perfusion Protein protocol purification Recombinant spike protein responsible robust S-protein SARS CoV-2 SARS-CoV-2 similarity spike (S) glycoprotein Spike protein STABLE surface protein surface proteins the receptor-binding domain the spike protein therapeutic drug trimeric variant viral envelope proteins virus β-coronavirus [DOI] 10.3389/fbioe.2021.779359 PMC 바로가기 [Article Type] Bioengineering and Biotechnology
A Linkage-specific Sialic Acid Labeling Strategy Reveals Different Site-specific Glycosylation Patterns in SARS-CoV-2 Spike Protein Produced in CHO and HEK Cell SubstratesChemistry Published on 2021-09-242022-10-31 Journal: Frontiers in Chemistry [Category] COVID-19, [키워드] ACE2 acid acute respiratory syndrome amidation ammonium hydroxide C-terminal trimerization motif cell substrate cells Chinese hamster CHO cell complex coronavirus determine diagnostics dimethylamine embryonic kidney EThcD evaluate exhibit Express glycan glycan shield glycoproteomics glycosylated glycosylation HEK cell high-mannose Host host immune system immune system Interaction modify N-glycosylation N-linked glycosylation pattern predicted presenting produced profile Protein reaction receptor recombinant S protein Research reveal S protein SARS-CoV-2 sialic acid site occupancy spike Spike protein Spike proteins Strategy substrate Support suspensions the S protein the SARS-CoV-2 These data transmembrane domain Vaccines viral envelope virus [DOI] 10.3389/fchem.2021.735558 PMC 바로가기 [Article Type] Chemistry
Coronavirus Pseudotypes for All Circulating Human Coronaviruses for Quantification of Cross-Neutralizing Antibody Responses교차 중화 항체 반응의 정량화를위한 모든 순환 인간 코로나 바이러스에 대한 코로나 바이러스 의사 타입Article Published on 2021-08-102022-09-01 Journal: Viruses [Category] MERS, SARS, 진단, [키워드] ACE2 antibody antibody cross-reactivity Antibody neutralization best Betacoronavirus breadth can be used Cell cell line cells CHO cell CHO cells Clinical outcome condition coronavirus COVID-19 COVID-19 disease COVID-2019 cross-reactivity disease Disease progression Effect Endemic envelope Haemagglutinin HCoV HCoV-229E HCoV-HKU1 HCoV-NL63 HCoV-OC43 HCoVs HEK293T HKU1 HUH7 cells Human human coronavirus Immunity immunopathology lentiviral MERS MERS-CoV natural infection neutralization Neutralizing NL63 Novel coronavirus novel coronavirus SARS-CoV-2 OC43 pre-existing immunity produced progression Protein Proteins provide pseudotype pseudotype virus pseudotype viruses Pseudotyped virus Research question response Safe SARS-CoV-1 SARS-CoV-2 seasonal coronavirus serological Seropositivity Seven surface proteins target cell target cells the SARS-CoV-2 titre TMPRSS2 TMPRSS2 expression transduced transfected with understanding vaccination viruses [DOI] 10.3390/v13081579 PMC 바로가기 [Article Type] Article
Evaluation of Anti-PT Antibody Response after Pertussis Vaccination and Infection: The Importance of Both Quantity and Quality백일해 백신 접종 및 감염 후 항-PT 항체 반응 평가: 양과 질의 중요성Review Published on 2021-07-212024-07-28 Journal: Toxins [Category] 백일해, [키워드] antibodies avidity B cells CHO cell Epitopes Pertussis toxin [DOI] 10.3390/toxins13080508 PMC 바로가기 [Article Type] Review
A simple, sensitive, and low‐cost FACS assay for detecting antibodies against the native SARS‐CoV‐2 spike protein천연 SARS-CoV-2 스파이크 단백질에 대한 항체를 검출하기 위한 간단하고 민감하며 저렴한 FACS 분석Original Article Published on 2021-05-122022-09-10 Journal: Immunity, Inflammation and Disease [Category] SARS, 진단, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus antibodies antibody Antibody Response Antibody responses Antibody titer Antibody titers applied Asymptomatic Care CHO cell CHO cells coronavirus COVID‐19 COVID‐19 patient COVID‐19 patients detectable develop Disease progression donors Epidemic expression vector expression vectors FACS flow cytometry Germany GFP Hamburg Health Health care HEK cell help hospital hospitalized patients household contacts human serum identify individual Infectious diseases Infectious virus lab Microscopy MONITOR nuclear Protein Recombinant protein recombinant proteins remained respiratory responses SARS‐CoV‐2 secondary antibody social distancing spike Spike protein Symptom symptomatic contacts the epidemic transfected cell transfected cells trials utility vaccination Virology virus [DOI] 10.1002/iid3.446 PMC 바로가기 [Article Type] Original Article
High-level expression of the monomeric SARS-CoV-2 S protein RBD 320-537 in stably transfected CHO cells by the EEF1A1 -based plasmid vectorEEF1A1 기반 플라스미드 벡터에 의한 안정적으로 형질감염된 CHO 세포에서 단량체 SARS-CoV-2 S 단백질 RBD 320-537의 고수준 발현Research Article Published on 2021-02-022022-08-13 Journal: PLoS ONE [Category] Biochemistry, COVID-19, [키워드] Accuracy acute respiratory syndrome Alpha amplification analyzed Antigen antigenic antigenic determinants C-terminal Cell culture cell line Chinese hamster CHO cell condition coronavirus coronavirus 2 Coronaviruses described determinant domain EEF1A1 elongation factor exceeded except for expression gel gel filtration Genetic Genome glycans glycosylation Host immune response inducer mammalian mass spectrometry methotrexate monomeric N-glycosylation peptide performed Plasmid PNGase F polyacrylamide polyacrylamide gel produced Protein purification RBD RBD protein recombinant Recombinant SARS-CoV-2 RBD reducing regulatory sequence regulatory sequences respiratory S protein S proteins SARS-CoV-2 SARS-CoV-2 RBD SARS-CoV-2 S protein selected serological serological test Serological tests severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 signal peptide subunit Subunit vaccine the S protein transfected cells translation unique Vaccine variant Viral viral envelope viral signal was obtained [DOI] 10.1371/journal.pone.0242890 PMC 바로가기 [Article Type] Research Article
Recombinant SARS-CoV-2 spike S1-Fc fusion protein induced high levels of neutralizing responses in nonhuman primatesArticle Published on 2020-07-312022-10-29 Journal: Vaccine [Category] COVID-19, [키워드] antibody production Antibody titer CHO cell confirmed case COVID-19 COVID-19 outbreak COVID-19 patient COVID-19 vaccine death evidenced examined expressing fusion protein global pandemic immunized immunogenic induce Infection injection live SARS-CoV-2 Live virus mice MOST Neutralizing activity neutralizing response Protein Pseudovirus neutralization assay recombinant responsible SARS-CoV-2 SARS-CoV-2 spike Spike protein STABLE Strong vaccination Vaccine Vaccine development virus virus neutralizing titer [DOI] 10.1016/j.vaccine.2020.06.066 PMC 바로가기 [Article Type] Article