Highly Sensitive Lineage Discrimination of SARS-CoV-2 Variants through Allele-Specific Probe PCR대립유전자 특이적 프로브 PCR을 통한 SARS-CoV-2 변이체의 고감도 계통 판별Article Published on 2022-04-202022-09-11 Journal: Journal of Clinical Microbiology [Category] COVID19(2023년), SARS, 변종, 치료기술, 치료제, [키워드] adopted allele allele-specific probe PCR alternative method alternative methods Brazil clinical trial cohorts Comparative advantage COVID-19 therapeutics cycle threshold Degradation detect diagnostics discrimination effort Evidence evidence of Evolution Future future work gold gold standard high accuracy identify individual likelihood lineages Next-generation sequencing NGS nucleotide PCR probe public health Regression model required Result RNA SARS-CoV-2 SARS-CoV-2 lineage SARS-CoV-2 variant SARS-CoV-2 variants screened sensitive shown Single nucleotide polymorphism SNP SNPs Support United Kingdom Vaccines variant Variant identification variants variants of concern Viral load viral loads virus [DOI] 10.1128/jcm.02283-21 PMC 바로가기 [Article Type] Article
A cell-based ELISA as surrogate of virus neutralization assay for RBD SARS-CoV-2 specific antibodiesRBD SARS-CoV-2 특이적 항체에 대한 바이러스 중화 분석의 대리인 세포 기반 ELISAArticle Published on 2022-03-182022-09-11 Journal: Vaccine [Category] COVID19(2023년), MERS, SARS, 진단, 치료기술, [키워드] alternative methods biosafety level Biosafety Level 3 BSL2 BSL3 Cell cell-based ELISA correlation COVID-19 pandemic cVNT death detect determine Efficacy ELISA gold gold standard immune response Inhibition assays Laboratory level 3 molecular mVNT. neutralization Neutralization assay neutralization test performed RBD Safe SARS-CoV-2 SARS-CoV-2 RBD SARS-CoV-2 RBD binding SARS-CoV-2 specific antibody sensitivity serological Serological assay species specificity surrogate Vaccine Viral viral neutralization Virus neutralization virus neutralization assays [DOI] 10.1016/j.vaccine.2022.02.044 PMC 바로가기 [Article Type] Article
COVID-19 disease diagnosis from paper-based ECG trace image data using a novel convolutional neural network model새로운 컨볼루션 신경망 모델을 사용하여 종이 기반 ECG 추적 이미지 데이터에서 COVID-19 질병 진단Article Published on 2022-03-012022-09-11 Journal: Physical and engineering sciences in medicine [Category] SARS, 치료기술, [키워드] Abnormalities abnormality Accuracy addition alternative method alternative methods AUC value binary classification cardiovascular cardiovascular diseases Cardiovascular diseases diagnosis cardiovascular system caused clinical clinician Convolutional neural network Convolutional neural networks COVID-19 COVID-19 diagnosis COVID-19 disease COVID-19 infected patient COVID-19 infected patients COVID-19 pandemic COVID-19 patients COVID-19 virus deep deep learning detect Diagnosis diagnosis of COVID-19 Diagnostic method disease ECG Electrocardiography heartbeat Impact infected patients limitations machine machine learning Medical imaging multi-classification Myocardial Myocardial infarction network model Neural normal pandemic PCR tests Respiratory system RT-PCR test shown Treatment treatment of COVID-19 patients [DOI] 10.1007/s13246-022-01102-w PMC 바로가기 [Article Type] Article
In Silico Prediction and Selection of Target Sequences in the SARS-CoV-2 RNA Genome for an Antiviral AttackArticle Published on 2022-02-142022-10-28 Journal: Viruses [Category] COVID-19, COVID19(2023년), Fulltext, SARS, [키워드] 229E alternative methods analyzed Antiviral antiviral drugs Antiviral strategies antiviral strategy approaches attack conserved contribute Coronaviruses COVID-19 CRISPR-Ca CRISPR-Cas13d effective HKU1 human coronavirus humans MERS-CoV Molecular biology NL63 nucleotide OC43 omicron outbreak Pandemics performed prediction protective vaccine provide related viruses replicate RNA RNA interference Safe SARS-CoV SARS-CoV-2 SARS-CoV-2 genome SARS-CoV-2 isolates SARS-CoV-2 pandemic screened Selection sequence target the SARS-CoV-2 the SARS-CoV-2 genome unique variant [DOI] 10.3390/v14020385 PMC 바로가기 [Article Type] Article
An ultrafast SARS-CoV-2 virus enrichment and extraction method compatible with multiple modalities for RNA detectionRNA 검출을 위한 다중 양식과 호환되는 초고속 SARS-CoV-2 바이러스 농축 및 추출 방법Randomized Controlled Trial Published on 2021-10-022022-09-11 Journal: Analytica chimica acta [Category] MERS, SARS, 임상, 진단, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus affinity Affinity nanoparticles alternative method alternative methods amplification Analysis Chain Reaction characterization characterized clinical samples clinically comparable compatibility Concordance coronavirus Coronavirus-2 COVID-19 pandemic demonstrated diagnoses Dilution dilutions double-blind study Enzymatic extraction form isothermal amplification Laboratory LAMP Loop-mediated amplification (LAMP) Loop-mediated isothermal amplification miniaturization nasopharyngeal swabs Particle pathogenicity polymerase polymerase chain polymerase chain reaction Polymerase chain reaction (PCR) positive recombinase polymerase amplification Recombinase polymerase amplification (RPA) respiratory reverse transcription reverse transcription real-time polymerase chain reaction RNA RNA eluate RNA isolation RNA virus RPA RT-PCR Saliva SARS-CoV-2 SARS-CoV-2 viral SARS-CoV-2 virus Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 severe acute respiratory syndrome coronavirus-2 Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) solution Stage the stage transmission rate Viral viral titer viral titers viral transport media VTM zoonotic [DOI] 10.1016/j.aca.2021.338846 PMC 바로가기 [Article Type] Randomized Controlled Trial
Validation of conventional PCR-like alternative to SARS-CoV-2 detection with target nucleocapsid protein gene in naso-oropharyngeal samples나스-오만 핵 샘플에서 표적 뉴 클레오 캡시드 단백질 유전자에 의한 SARS-COV-2 검출에 대한 종래의 PCR- 유사 대안의 검증Research Article Published on 2021-09-232022-09-10 Journal: PLoS ONE [Category] SARS, 진단, 치료기술, 치료법, 치료제, [키워드] Accuracy alternative alternative method alternative methods bioinformatics can be used condition conditions Conventional correlation coefficient correlations Detection limit develop Diagnosis diagnostic test diagnostic tests epidemiological studies Epidemiological study expected generate Health health problem Infection N gene naso-oropharyngeal sample naso-oropharyngeal swab nucleocapsid protein Patient positive control primer product qPCR RT-PCR SARS-CoV-2 SARS-CoV-2 detection sensitivity specificity Spread validation was determined [DOI] 10.1371/journal.pone.0257350 PMC 바로가기 [Article Type] Research Article
Comparison of the Modified Centers for Disease Control and Prevention 2019-Novel Coronavirus Real-Time RT-PCR Method for Detection of Infectious and Heat-Inactivated Virus on Stainless Steel수정된 질병통제예방센터 2019-신종 코로나바이러스 실시간 RT-PCR 방법으로 스테인리스강의 감염성 및 열불활성화 바이러스 검출 비교Article Published on 2021-08-202022-09-11 Journal: Journal of AOAC International [Category] SARS, 치료기술, [키워드] 2019-nCoV Aliquots alternative method alternative methods analyzed biosafety level BSL-3 facility CDC center chain Chain Reaction Control demonstrated detection disease control Emergency expected extension genomic heat heat-inactivated Highlights in viral infectious Infectious virus Level medium mitigate no difference Novel coronavirus Panel POD polymerase chain reaction positive result prevention Probability qPCR reaction Real time reverse transcription polymerase chain reaction respiratory response Result resulting RT-PCR RT-qPCR SARS-CoV-2 severe acute respiratory syndrome coronavirus-2 stainless steel statistical steel Study design Test time Transport transport medium validation viral stocks Viral transport medium virus virus detection VTM was used [DOI] 10.1093/jaoacint/qsab046 PMC 바로가기 [Article Type] Article
Modelling conformational state dynamics and its role on infection for SARS-CoV-2 Spike protein variants모델링 형태 상태 역학 및 SARS-COV-2 스파이크 단백질 변이체에 대한 감염에 대한 역할Research Article Published on 2021-08-052022-08-13 Journal: PLoS Computational Biology [Category] MERS, SARS, 변종, 유전자 메커니즘, [키워드] 501.V2 ACE2 alternative methods B.1.1.7 calculate cell entry computational method conformational COVID-19 D614G decrease facilitate flexibility glycine helping human ACE2 receptor identify increase in Infection K417 knowledge less matter mechanism molecular mechanism molecular mechanisms multiple mutation multiple mutations Mutation N501Y N501Y mutation new strain normal mode analysis open Pandemics predict proof proof of concept Protein public health residue residues resource SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 spike protein several variant several variants Simulation specific mutation specific mutations spike Spike protein Strains Surveillance the SARS-CoV-2 the Spike transition probabilities transition probability treat variant variants Viral viral entry virus [DOI] 10.1371/journal.pcbi.1009286 PMC 바로가기 [Article Type] Research Article
Eating Motives and Other Factors Predicting Emotional Overeating during COVID-19 in a Sample of Polish Adults폴란드 성인 표본에서 COVID-19 기간 동안 정서적 과식을 예측하는 섭식 동기 및 기타 요인Clinical Trial Published on 2021-05-132022-08-13 Journal: Nutrients [Category] SARS, 임상, [키워드] activity Affect affect regulation age alternative method alternative methods BMI Contact contact with COVID-19 COVID-19 pandemic COVID-19-related stress demographic variable Demographic variables dominant eating eating motives Effect emotional emotional overeating Factor Final Gender generate Health help hunger hypothesised individual lead mechanism motivation negative emotion negative emotions Other overeating participant patients predictor R 2 Regression Regulation resource resulting Sample significantly social contact socio-demographic survey Stress survey weight control weight control) [DOI] 10.3390/nu13051658 PMC 바로가기 [Article Type] Clinical Trial
An integrated autoencoder-based hybrid CNN-LSTM model for COVID-19 severity prediction from lung ultrasound폐 초음파에서 COVID-19 심각도 예측을 위한 통합 자동 인코더 기반 하이브리드 CNN-LSTM 모델Article Published on 2021-05-012022-09-11 Journal: Computers in biology and medicine [Category] SARS, 치료기술, [키워드] alternative method alternative methods Analysis average build CNN Convolutional neural network COVID-19 COVID-19 pandemic COVID-19 severity creating deep deep learning Diagnosis disease severity effective Efficacy feature flexible fused global healthcare integration Long lung Lung ultrasound Neural Neural network performed predict Safe Severity Score Short-Term Memory the patient threat Ultrasound [DOI] 10.1016/j.compbiomed.2021.104296 PMC 바로가기 [Article Type] Article