Use of a Bacterial Artificial Chromosome to Generate Recombinant SARS-CoV-2 Expressing Robust Levels of Reporter GenesArticle Published on 2022-12-212023-07-10 Journal: Microbiology Spectrum [Category] COVID19(2023년), [키워드] BAC bacterial artificial chromosome coronavirus COVID-19 pBeloBAC recombinant virus reporter genes reverse genetics SARS-CoV-2 [DOI] 10.1128/spectrum.02732-22 PMC 바로가기
Streptomyces BAC Cloning of a Large-Sized Biosynthetic Gene Cluster of NPP B1, a Potential SARS-CoV-2 RdRp InhibitorArticle Published on 2022-07-282023-07-09 Journal: The Cochrane database of systematic reviews [Category] COVID19(2023년), [키워드] bacterial artificial chromosome biosynthetic gene cluster. Nystatin-like polyene SARS-CoV-2 RdRp [DOI] 10.4014/jmb.2205.05036 PMC 바로가기
Of mice and human-specific long noncoding RNAs마우스 및 인간 특이적 긴 비암호화 RNAReview Published on 2022-06-012022-09-11 Journal: Mammalian genome : official journal of the Interna [Category] COVID19(2023년), SARS, 유전자 메커니즘, [키워드] ACE2 gene acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 approach BAC Bacterial bacterial artificial chromosome Cell culture cell culture system cell culture systems chromosome complement conducted coronavirus coronavirus disease Coronavirus disease-19 COVID-19 effort ENCODE etiologic agent example exceeded Genome highlight in vivo integration lncRNA long noncoding RNA mechanism mice MOST mouse offer pandemic protein-coding genes random receptor Regulation SARS-CoV-2 severe acute respiratory syndrome Coronavirus upstream Vascular [DOI] 10.1007/s00335-022-09943-2 PMC 바로가기 [Article Type] Review
Development of Robust Varicella Zoster Virus Luciferase Reporter Viruses for In Vivo Monitoring of Virus Growth and Its Antiviral Inhibition in Culture, Skin, and Humanized Mice강력한 수두대상포진 바이러스 루시페라제 리포터 바이러스의 개발: 배양, 피부 및 인간화 마우스에서 바이러스 성장 및 항바이러스 억제의 인 비보 모니터링을 위한Article Published on 2022-04-152023-05-31 Journal: Viruses [Category] 대상포진, 두창, 수두, 홍역, [키워드] antiviral drugs bacterial artificial chromosome bioluminescence imaging humanized mice luciferase skin organ culture Varicella-Zoster Virus [DOI] 10.3390/v14040826 PMC 바로가기
Establishment of a stable SARS-CoV-2 replicon system for application in high-throughput screening고처리량 스크리닝 적용을 위한 안정적인 SARS-CoV-2 레플리콘 시스템 구축Article Published on 2022-03-012022-09-11 Journal: Antiviral Research [Category] SARS, 치료제, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus Antiviral Antiviral effect antiviral effects Bacterial bacterial artificial chromosome biosafety level Biosafety Level 3 BSL2 BSL3 camostat Cell cell line cell lines cells chromosome compounds condition conditions coronavirus COVID-19 examined exhibited Favipiravir gene encoding In vitro infection inhibited interferon level 3 luciferase luciferase activity molnupiravir Neomycin Novel coronavirus Particles powerful tool produced RdRP RdRp inhibitor Remdesivir Renilla Renilla luciferase replicon reported RNA SARS-coronavirus SARS-coronavirus-2 SARS-CoV-2 severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 sgRNA sgRNAs stable cell stable cell line suppressed the SARS-CoV-2 transient transfection transiently transfected Treatment VeroE6 cells Viral proteins viral replication [DOI] 10.1016/j.antiviral.2022.105268 PMC 바로가기 [Article Type] Article
A Convenient and Biosafe Replicon with Accessory Genes of SARS-CoV-2 and Its Potential Application in Antiviral Drug DiscoverySARS-CoV-2의 보조 유전자와 항바이러스 약물 발견에 대한 잠재적 응용을 포함하는 편리하고 생물학적으로 안전한 복제품Article Published on 2021-10-012022-09-11 Journal: Virologica Sinica [Category] SARS, 치료제, [키워드] absence Accessory Analysis anti-SARS-CoV-2 antiviral drug Antiviral drug screening antiviral drugs application BAC Bacterial bacterial artificial chromosome benefit biosafety level Biosafety Level 3 BSL3 can be used cause cDNA Cell chromosome Containment costly Deletion disease drug effective drug effective drugs exonuclease expression genetics Genome genome replication Infection infectious inhibition assay Inhibition assays inhibitors Laboratory level 3 Live virus luciferase reporter gene methyltransferase Nsp12 nsp14 pandemic of COVID-19 pathogenicity Potential profile Replication replicon reporter gene Research research and development reverse genetics RNA RNA polymerase S protein SARS-CoV-2 sgRNA subgenomic RNA subgenomic RNAs this system transfection viral cDNA virion assembly virus wild-type virus [DOI] 10.1007/s12250-021-00385-9 PMC 바로가기 [Article Type] Article
Cloning of a Passage-Free SARS-CoV-2 Genome and Mutagenesis Using Red Recombination무계대 SARS-CoV-2 게놈의 복제 및 적색 재조합을 사용한 돌연변이 유발Article Published on 2021-09-222022-09-10 Journal: International Journal of Molecular Sciences [Category] SARS, 치료기술, 치료법, [키워드] acute respiratory syndrome Antiviral applied approach BAC Bacterial bacterial artificial chromosome Biology biology of SARS-CoV-2 caused Cell culture chromosome click chemistry clone Cloning Combination concerning contribute coronavirus CoV COVID-19 COVID-19 pandemic disease economic devastation facilitate Features FLAG Frame full-length generate Genetic global effort global efforts incoming viral particles labeling Lambda marker morbidity Mortality nucleocapsid Open reading frame pandemic coronavirus platform powerful tool question recombinant virus Recombination Red red recombination replicated Replication reporter reporter virus respiratory reverse genetics SARS-CoV-2 Severe acute respiratory syndrome Severe Acute Respiratory Syndrome CoV single-cell level spatiotemporal Spread Viral viral particle viral replication Viral RNA viral RNA synthesis virus viruses Visualization [DOI] 10.3390/ijms221910188 PMC 바로가기 [Article Type] Article
Identification of four insertion sites for foreign genes in a pseudorabies virus vectorResearch Published on 2021-05-122022-10-28 Journal: BMC veterinary research [Category] Coronavirus, [키워드] Affect antibody BAC Bacterial bacterial artificial chromosome Cell chromosome epidemic diarrhea exhibited expression expression cassette Gene Expression Genome growth identification immunofluorescence insertion insertion site mRNA expression mutant Noncoding region parental PCR post infection Pseudorabies virus Pseudoraby Real-time PCR recovered Result S gene Safe shown spike gene Vaccine Vaccines variant virulent virus virus strain Virus vector [DOI] 10.1186/s12917-021-02887-w PMC 바로가기 [Article Type] Research
A bacterial artificial chromosome (BAC)-vectored noninfectious replicon of SARS-CoV-2SARS-CoV-2의 박테리아 인공 염색체(BAC) 벡터 비감염성 복제Article Published on 2021-01-012022-09-11 Journal: Antiviral Research [Category] SARS, 치료제, [키워드] Antiviral antiviral agent Antiviral agents antiviral protein BAC Bacterial bacterial artificial chromosome biosafety biosafety level cell lines chromosome COVID-19 pandemic daclatasvir desired mutation desired mutations envelope facilitate hepatitis C hepatitis C virus IFN-α in viral In-vitro inhibitor Interferon alpha membrane nCoV overexpression Plasmid Protein Remdesivir replicated Replication replicon reporter reporter gene RNA SARS-CoV-2 secreted sofosbuvir structural genes subgenomic mRNA transfection Viral viral subgenomic mRNA Virus-host interactions ZAP Zinc [DOI] 10.1016/j.antiviral.2020.104974 PMC 바로가기 [Article Type] Article
Generation of Recombinant SARS-CoV-2 Using a Bacterial Artificial ChromosomeArticle Published on 2020-12-012023-07-13 Journal: Curr Protoc Microbiol [Category] SARS, [키워드] bacterial artificial chromosome coronavirus COVID-19 infectious clone reverse genetics SARS-CoV-2 virus rescue. [DOI] 10.1002/cpmc.126 PMC 바로가기