A new-type HOCl-activatable fluorescent probe and its applications in water environment and biosystems새로운 유형의 HOCl 활성화 가능한 형광 프로브 및 물 환경 및 생물 시스템에서의 응용Article Published on 2022-09-152022-09-11 Journal: The Science of the total environment [Category] 치료기술, 치료제, [키워드] Accuracy Affect aggregate assistance Biotin biotinylated Cancer cancer cell Cancer cells cancer diagnosis Cell cells chlorine Concentration Consumption contribute convenience Corona COVID-19 Early Cancer expected Fluorescence fluorescent Fluorescent probe Health HOCl human body hypochlorite hypochlorous acid inflammatory disease Inflammatory diseases introduced kill membrane outbreak overexpressed oxygen PBS Precision probe reactive oxygen specy receptor ROS significant increase sodium sodium hypochlorite Spread Virus Disease viruses water Water samples. while [DOI] 10.1016/j.scitotenv.2022.156164 PMC 바로가기 [Article Type] Article
Electrochemical genosensor for the specific detection of SARS-CoV-2SARS-CoV-2의 특정 검출을 위한 전기화학적 제노센서Article Published on 2022-08-012022-09-11 Journal: Talanta [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] alternative biotinylated capture caused cell cultures complex complexes coronavirus disease COVID-19 cultures detect Diagnosis diagnostic discrimination disease DNA-RNA followed by Genosensor homologous horseradish peroxidase imperative in vitro Increases limit of detection maleimide molecular mortality rate Nanobioconjugate pandemic Particles peroxidase probe probes respiratory respiratory viruses responsible SARS-CoV-2 SARS-CoV-2 cell SARS-CoV-2 detection sensitivity serological test Serological tests Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus-2 Signaling streptavidin Surveillance tested the patient virus [DOI] 10.1016/j.talanta.2022.123482 PMC 바로가기 [Article Type] Article
Multiplexed flow cytometric approach for detection of anti-SARS-CoV-2 IgG, IgM and IgA using beads covalently coupled to the nucleocapsid protein뉴클레오캡시드 단백질에 공유 결합된 비드를 사용하여 항-SARS-CoV-2 IgG, IgM 및 IgA의 검출을 위한 다중 유동 세포측정 접근법Article Published on 2022-06-012022-09-11 Journal: Letters in Applied Microbiology [Category] COVID19(2023년), MERS, SARS, 진단, 치료기술, 치료법, [키워드] anti-IgG anti-IgM anti-SARS-CoV-2 IgG antibody approach biotinylated CBA CBA functional beads condition COVID-19 COVID-19 negative Cytometry Diagnosis Flow flow cytometric flow cytometry functional IgA IgA antibodies IgA2 IgG IgM IgM and IgA antibodies immunoassay Immunoglobulin Immunoglobulin G multiplex multiplex immunoassay. nucleocapsid protein opens positive Protein SARS-CoV-2 SARS-CoV-2 antibodies SARS-CoV-2 nucleocapsid secondary antibodies sensitivity specificity streptavidin were used [DOI] 10.1111/lam.13674 PMC 바로가기 [Article Type] Article
An antibody-based proximity labeling protocol to identify biotinylated interactors of SARS-CoV-2SARS-CoV-2의 비오틴화된 상호작용자를 식별하기 위한 항체 기반 근접 라벨링 프로토콜Article Published on 2022-05-022022-09-12 Journal: STAR protocols [Category] COVID19(2023년), MERS, SARS, 진단, [키워드] Analysis approach biotinylated Complete Host identify labeling mass mass spectrometry mechanism Microbiology molecular interaction Molecular/Chemical Probes non-specific peptide Protein Biochemistry Proteins proteomics protocol SARS-CoV-2 SARS-CoV-2 protein viral pathogenesis Viral proteins virus [DOI] 10.1016/j.xpro.2022.101406 PMC 바로가기 [Article Type] Article
An engineered nano-liposome-human ACE2 decoy neutralizes SARS-CoV-2 Spike protein-induced inflammation in both murine and human macrophages조작된 나노 리포솜 인간 ACE2 미끼는 쥐와 인간 대식세포 모두에서 SARS-CoV-2 스파이크 단백질 유도 염증을 중화합니다Article Published on 2022-03-062022-09-11 Journal: Theranostics [Category] SARS, 유전자 메커니즘, [키워드] abrogate abrogated ACE2 activating Activation acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Analysis angiotensin Angiotensin-converting enzyme angiotensin-converting enzyme 2 binding biotinylated canonical CCL-2 chemokines competitive inhibitor complex coronavirus CXCL-10 cytokine D614G deficiency DEGs demonstrated differentially expressed gene Effect enzyme expressing expression fusion generate glycoprotein hACE2 human ACE2 human host cells human macrophage human macrophages IKKβ IKKβ/NF-κB signaling IL-1β IL-6 Immune cell immune cells in vitro in vivo Infection Inflammation Inflammatory response Inflammatory responses inhibit SARS-CoV-2 inhibitor internalization Intravenous injection IκB lentivirus Liposome Liposome-Human ACE2 Macrophage macrophages mechanism mice molecular murine myeloid-specific IKKβ knockout. neutralize NF-κB NF-κB activation NF-κB signaling Protein rationale receptor RNA sequencing SARS-CoV-2 SARS-CoV-2 spike glycoprotein severe acute respiratory syndrome Coronavirus Signaling spike spike glycoprotein Spike protein stimulated Therapeutic approach tissue inflammation TNFα Treatment was used wild type [DOI] 10.7150/thno.66831 PMC 바로가기 [Article Type] Article
Development and utilization of a surrogate SARS-CoV-2 viral neutralization assay to assess mRNA vaccine responsesmRNA 백신 반응을 평가하기 위한 대리 SARS-CoV-2 바이러스 중화 분석의 개발 및 활용Research Article Published on 2022-01-182022-09-12 Journal: PLoS ONE [Category] 진단, [키워드] angiotensin angiotensin converting enzyme biosafety biotinylated booster Booster vaccine Complete configuration correlation COVID COVID-19 COVID-19 patient COVID-19 patients Decline declines development doses ELISA ELISAs enzyme evaluate gold gold standard help heterogeneous highlight Highlights host receptor immunoassay Infection inhibitory activity Interaction Longitudinal analyses longitudinal analysis mRNA vaccine Neutralization assay neutralization test Neutralizing activity plaque reduction neutralization Plaque reduction neutralization test post vaccination PRNT raise RBD receptor Receptor binding domain recipient Recovered COVID-19 patients response SARS-CoV-2 immunity SARS-CoV-2 spike SARS-CoV-2 viral serum Serum neutralizing antibody significant difference significant differences significantly higher Spike protein Test tested vaccinated individual vaccination Vaccine vaccine response vaccine responses viral culture was used within 6 months [DOI] 10.1371/journal.pone.0262657 PMC 바로가기 [Article Type] Research Article
Proximity-dependent biotinylation detects associations between SARS coronavirus nonstructural protein 1 and stress granule–associated proteins근접 의존성 비오틴화는 SARS 코로나바이러스 비구조 단백질 1과 스트레스 과립 관련 단백질 사이의 연관성을 감지합니다Research Article Published on 2021-11-112022-09-10 Journal: The Journal of Biological Chemistry [Category] MERS, SARS, 유전자 메커니즘, 치료기술, [키워드] α-CoV, α-coronavirus β-CoV, β-coronavirus acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addition Amino acid amino acids Analysis assembly association BioID2 biotinylated biotinylated proteins BSA, bovine serum albumin C-terminal cellular coronavirus Critical detect disrupt eIF, eukaryotic translation initiation factor Exosome Factor FDR, false discovery rate followed by G3BP1 G3BP1, Ras GTPase-activating protein SH3 domain–binding protein 1 HA, hemagglutinin HEK293, human embryonic kidney 293 cell line Host host cell host gene expression identify interact Interaction maturation mechanism MERS-CoV, Middle Eastern respiratory syndrome coronavirus mRNAs Mutation nonstructural protein 1 nsp1 nsp1, nonstructural protein 1 observé pre-mRNA Protein Proteins proteomic respiratory ribosome SARS Coronavirus SARS-CoV SARS-CoV-2 SARS-CoV-2, Severe acute respiratory syndrome coronavirus 2 SARS-CoV, severe acute respiratory syndrome coronavirus severe acute respiratory syndrome Coronavirus SG, stress granule sodium sodium arsenite Stress stress granule suppresse Transcription Viral Viral protein [DOI] 10.1016/j.jbc.2021.101399 PMC 바로가기 [Article Type] Research Article
An antibody-based proximity labeling map reveals mechanisms of SARS-CoV-2 inhibition of antiviral immunityResearch article Published on 2021-10-222022-10-05 Journal: Cell chemical biology [Category] SARS, 치료제, [키워드] ACE2 Activation acute respiratory syndrome angiotensin-converting enzyme 2 Antiviral antiviral immunity biotinylated caused Clinical treatment Compound coronavirus coronavirus disease Coronavirus-2 COVID-19 domain drug Epidemic Human human cell humans identify immune responses Infection inhibit Interaction interactome ITGB1 knowledge labeling lysine MAVS mechanism membrane protein methyltransferase mitochondrial mitochondrial antiviral-signaling protein pathway Protein provide proximal proximity labeling replication of SARS-CoV-2 resource reveal SARS-CoV-2 SARS-CoV-2 entry SARS-CoV-2 protein SET SETD2 significantly the interferon Treatment TurboID viral infection Viral protein [DOI] 10.1016/j.chembiol.2021.10.008 [Article Type] Research article
Catalytic hairpin DNA assembly-based chemiluminescent assay for the detection of short SARS-CoV-2 target cDNA짧은 SARS-CoV-2 표적 cDNA 검출을 위한 촉매 헤어핀 DNA 어셈블리 기반 화학발광 분석Article Published on 2021-10-012022-09-11 Journal: Talanta [Category] SARS, 변종, 치료기술, 치료제, [키워드] amplify approach Biotin biotinylated catalytic Catalytic hairpin assembly cDNA challenge Chemiluminescent assay color change colorimetric detection Combination complex Concentration detect Detection limit Detection sensitivity diagnostics dimeric DNA DNA probe enzyme enzyme-linked immunosorbent enzyme-linked immunosorbent assay equipment exhibit horseradish peroxidase IMPROVE interactions mutant mutant gene naked eye peroxidase powerful tool probe protocol resulting SARS-CoV-2 selective sensitivity sequence specificity target Target DNA. target gene Target genes [DOI] 10.1016/j.talanta.2021.122505 PMC 바로가기 [Article Type] Article
Single-Domain Antibodies for the Detection of SARS-CoV-2 Nucleocapsid ProteinSARS-CoV-2 뉴클레오캡시드 단백질 검출을 위한 단일 도메인 항체Article Published on 2021-05-182022-09-11 Journal: Analytical Chemistry [Category] SARS, 치료기술, 치료법, [키워드] antibody assays assist biotinylated described detection Detection limit determined by develop domain Effectiveness ENhance epitope Epitopes evaluated expressed FIVE Genetic high affinity immune immunized incorporation llama microsphere multiplexed immunoassay nucleocapsid nucleocapsid protein pandemic platform Protein reagent SARS-CoV-2 SARS-CoV-2 nucleocapsid selected sequence surface plasmon surface plasmon resonance the SARS-CoV-2 unique utility virus [DOI] 10.1021/acs.analchem.1c00677 PMC 바로가기 [Article Type] Article