ACE2 decoy receptor generated by high-throughput saturation mutagenesis efficiently neutralizes SARS-CoV-2 and its prevalent variants고처리량 포화 돌연변이 유발에 의해 생성된 ACE2 미끼 수용체는 SARS-CoV-2 및 그 유행하는 변이체를 효율적으로 중화합니다Article Published on 2022-12-012022-09-11 Journal: Emerging Microbes & Infections [Category] COVID19(2023년), SARS, 변종, [키워드] ACE2 ACE2 binding ACE2-RBD binding approach binding binding affinity C-terminal cellular receptor changes in convalescent sera Critical critical point D614G decoy receptor domain Effect fused global pandemic glycosylation site High-throughput screening increase in Microarray monoclonal antibodies Mutagenesis mutations neutralization neutralize neutralized Omicron variant Point mutation prevalent protease Protein protein engineering Pseudotyped virus RBD RBD binding RBD-ACE2 receptor Receptor binding domain rescued SARS-CoV-2 SARS-CoV-2 virus significantly single-cell soluble ACE2 Spike protein the SARS-CoV-2 the SARS-CoV-2 virus therapeutic potential Treatment Vaccine variant variants viral entry viral spike protein wild-type [DOI] 10.1080/22221751.2022.2079426 PMC 바로가기 [Article Type] Article
Highly restricted SARS-CoV-2 receptor expression and resistance to infection by primary human monocytes and monocyte-derived macrophages고도로 제한된 SARS-CoV-2 수용체 발현 및 1차 인간 단핵구 및 단핵구 유래 대식세포에 의한 감염에 대한 내성Article Published on 2022-09-012022-09-11 Journal: Journal of leukocyte biology [Category] COVID19(2023년), SARS, 유전자 메커니즘, [키워드] ACE2 acute respiratory syndrome angiotensin Angiotensin-converting enzyme angiotensin-converting enzyme 2 cause caused cellular cellular receptor condition contribute coronavirus COVID-19 Defense direct infection enzyme Express expression facilitated global pandemic immune activation in vitro Infection involved Macrophage macrophages MDM mechanism monocyte monocyte-derived macrophage Monocyte-derived macrophages Monocytes myeloid cell myeloid cells occur Pathogenesis primary human monocyte priming pseudotyped virions receptor remained resistance to infection SARS-CoV-2 SARS-CoV-2 receptor SARS-CoV2 SARS-CoV2 infection Severe acute respiratory syndrome severity spike subtype the disease TMPRSS2 unlikely unpolarized viral spike [DOI] 10.1002/JLB.4COVA1121-579RR PMC 바로가기 [Article Type] Article
Antibody Avidity and Neutralizing Response against SARS-CoV-2 Omicron Variant after Infection or VaccinationArticle Published on 2022-08-312022-11-15 Journal: Journal of Immunology Research [Category] SARS, 변종, 진단, [키워드] adenovirus-based antibody Antibody avidity avidity bind binding binding antibody cellular receptor collected cross-protection domain dose elicited epitope exhibit Heterologous high affinity Immunity increase mRNA vaccine Mutation natural infection Neutralizing Neutralizing activity Neutralizing antibodies neutralizing antibody Neutralizing titer omicron pandemic patients Protective antibody RBD RBD-binding IgG remained response Sample SARS-CoV-2 SARS-CoV-2 infected patient SARS-CoV-2 spike protein subject tested the RBD the spike protein vaccination Vaccine vaccine dose vaccinees Vaccines variant virus wild-type wild-type SARS-CoV-2 wild-type virus [DOI] 10.1155/2022/4813199 PMC 바로가기
Selection of Bis-Indolyl Pyridines and Triphenylamines as New Inhibitors of SARS-CoV-2 Cellular Entry by Modulating the Spike Protein/ACE2 Interfaces스파이크 단백질/ACE2 인터페이스를 조절하여 SARS-CoV-2 세포 진입의 새로운 억제제로서 비스-인돌릴 피리딘 및 트리페닐아민 선택Article Published on 2022-08-162022-09-11 Journal: Antimicrobial Agents and Chemotherapy [Category] COVID19(2023년), SARS, 변종, [키워드] 50% effective concentration ACE2 acute respiratory syndrome acute respiratory syndrome coronavirus approaches association biochemical caused Cell cell lines cellular cellular receptor circulating circulating variant complex Compound coronavirus coronavirus disease COVID COVID-19 docking drug functional human cell lines identify in silico indicate Infection infectious agent inhibit inhibitor inhibitory inhibitory effect Interaction interface Lentiviral vector lentiviral vectors modulating molecular molecules New pandemic parameter pseudotyped pyridine receptor ACE2 S protein SARS-CoV-2 SARS-CoV-2 entry SARS-CoV-2 S protein SARS-CoV-2 strain screened selected Selection severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 spike susceptible the SARS-CoV-2 the Spike therapeutic purposes these compound these compounds triphenylamine variant viral entry viral infection viral replication virus wild-type [DOI] 10.1128/aac.00083-22 PMC 바로가기 [Article Type] Article
Evaluation of commercial Anti-SARS-CoV-2 neutralizing antibody assays in seropositive subjects혈청 양성 환자에서 상업용 Anti-SARS-CoV-2 중화 항체 분석 평가Article Published on 2022-07-012022-09-11 Journal: Journal of Clinical Virology [Category] COVID19(2023년), MERS, SARS, 변종, 진단, [키워드] absence added addition anti-RBD Anti-RBD antibodies Anti-RBD antibody anti-spike antibody anti-Spike IgG antibody Antibody titer Area bioMérieux cells cellular receptor Commercial tests Competitive anti-RBD immunoassays correlation difficulty functional highlight house immunoassay immunoassays immunochromatographic test Interaction marker NAb titer NAb titers neutralization test Neutralizing antibodies neutralizing antibody neutralizing antibody assay New P-value patients plaque reduction neutralization PRNT qualitative test Quantitative reduction SARS-CoV-2 SARS-CoV-2 entry Sensitivity and specificity sera seropositive subject seropositive subjects Spike protein surrogate surrogate markers Surrogate markers of protection. sVNT tested the spike protein threshold thresholds titer variants of concern virus neutralization test VNT VoC [DOI] 10.1016/j.jcv.2022.105169 PMC 바로가기 [Article Type] Article
A computational study of the interface interaction between SARS-CoV-2 RBD and ACE2 from human, cat, dog, and ferret인간, 고양이, 개 및 흰 족제비의 SARS-CoV-2 RBD와 ACE2 간의 인터페이스 상호 작용에 대한 컴퓨터 연구Article Published on 2022-07-012022-09-12 Journal: Transboundary and emerging diseases [Category] COVID19(2023년), MERS, SARS, 진단, [키워드] ACE2 acute respiratory syndrome Angiotensin-converting enzyme angiotensin-converting enzyme 2 animal model assist can be used cellular receptor classical coronavirus COVID-19 COVID-19 pandemic evaluate ferret help helping infection process inhibitor interacting residue Interaction molecular molecular dynamics Molecular dynamics simulation New coronavirus pathology performed Receptor binding domain residue SARS-CoV-2 RBD SARS-CoV-2 spike glycoprotein severe acute respiratory syndrome coronavirus 2 significant difference the spike protein [DOI] 10.1111/tbed.14234 PMC 바로가기 [Article Type] Article
Evidence for an ACE2-Independent Entry Pathway That Can Protect from Neutralization by an Antibody Used for COVID-19 TherapyCOVID-19 치료에 사용되는 항체에 의한 중화로부터 보호할 수 있는 ACE2 독립적 진입 경로에 대한 증거Article Published on 2022-06-282022-09-11 Journal: mBio [Category] COVID19(2023년), MERS, SARS, 변종, 진단, 치료기술, 치료제, [키워드] ACE2 ACE2 binding analyzed antibody Antibody-mediated neutralization blockade Can cell entry cell line cellular receptor COVID-19 COVID-19 patient COVID-19 therapy E484K Evidence hepatoma host cells Huh-7 Imdevimab Infection Interaction lung cell mechanism Mutation NCI-H1299 neutralization Neutralizing antibodies neutralizing antibody position PROTECT Protein rescued SARS-CoV-2 SARS-CoV-2 spike protein SARS-CoV-2 variants spike Spike protein the SARS-CoV-2 therapy vaccination variants of concern Vero Cell Viral viral entry virus VoC [DOI] 10.1128/mbio.00364-22 PMC 바로가기 [Article Type] Article
Glucocorticoids Bind to SARS-CoV-2 S1 at Multiple Sites Causing Cooperative Inhibition of SARS-CoV-2 S1 Interaction With ACE2글루코코르티코이드는 여러 부위에서 SARS-CoV-2 S1에 결합하여 ACE2와 SARS-CoV-2 S1 상호작용을 협력적으로 억제합니다Article Published on 2022-06-152022-09-11 Journal: Frontiers in Immunology [Category] COVID19(2023년), SARS, 변종, 진단, [키워드] ACE2 ACE2 (angiotensin converting enzyme 2) Affect Analysis angiotensin angiotensin converting enzyme angiotensin converting enzyme 2 anti-SARS-CoV-2 Asymptomatic Beta bind binding binding energy binding pocket binding pockets biochemical biochemically catalytic activity causing cellular receptor chimeric conformational state contributed Cooperative coronavirus cortisol COVID COVID-19 patients Delta Dexamethasone disrupt E484K enzyme Glucocorticoids indicated inhibition Innate immunity Interaction K417N Limited mass spectrometry mechanism Mild molecular molecular dynamics monoclonal antibody Multiple mutant mutated N501Y omicron Omicron variants Protein Receptor binding domain reduce mortality SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 S1 SARS-CoV-2 spike Site variant without affecting [DOI] 10.3389/fimmu.2022.906687 PMC 바로가기 [Article Type] Article
Cytoplasmic domain and enzymatic activity of ACE2 are not required for PI4KB dependent endocytosis entry of SARS-CoV-2 into host cellsSARS-CoV-2의 숙주 세포 내로의 PI4KB 의존성 세포내이입 진입에는 ACE2의 세포질 도메인 및 효소 활성이 필요하지 않습니다Article Published on 2022-06-012022-09-11 Journal: Virologica Sinica [Category] COVID19(2023년), SARS, 변종, [키워드] ACE2 addition angiotensin angiotensin converting enzyme angiotensin converting enzyme 2 Angiotensin converting enzyme 2 (ACE2) catalytic activity causative agent Cell cellular cellular entry cellular receptor clathrin Clathrin-mediated endocytosis COVID-19 pandemic delta variant demonstrated dependent on domain effective endocytosis entry of SARS-CoV-2 enzymatic activity enzyme health emergency host cell host cells human angiotensin converting enzyme 2 infections intracellular domain lentivirus Phosphoinositides PI4KB predominant principle pseudotype required SARS-CoV-2 Spike protein syncytium syncytium formation treat variant viral entry virus entry [DOI] 10.1016/j.virs.2022.03.003 PMC 바로가기 [Article Type] Article
A pair of noncompeting neutralizing human monoclonal antibodies protecting from disease in a SARS-CoV-2 infection modelSARS-CoV-2 감염 모델에서 질병으로부터 보호하는 한 쌍의 비경쟁 중화 인간 단일클론 항체Article Published on 2022-05-012022-09-11 Journal: European Journal of Immunology [Category] COVID19(2023년), MERS, SARS, 변종, 진단, [키워드] Analysis animals antibodies antibody binding candidate cellular receptor cellular receptor hACE2 Cluster competing COVID-19 disease DNA DNA sequence hACE2 human ACE2 human immunoglobulin human monoclonal antibodies Human monoclonal antibody IC50 Immunoglobulin mice N-terminal domain neutralize neutralized Neutralizing Neutralizing antibodies neutralizing antibody pandemic platform powerful tool Prophylaxis Receptor-binding domain reduced SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 mutant SARS-CoV-2 mutants SARS-CoV-2 neutralizing antibody SARS-CoV-2 spike protein selected Spike protein Support the receptor-binding domain the SARS-CoV-2 the spike protein therapy transgenic variants variants of concern Viral viral spread weight loss [DOI] 10.1002/eji.202149374 PMC 바로가기 [Article Type] Article