Pre-analytical sample stabilization by different sampling devices for PCR-based COVID-19 diagnosticsPCR 기반 COVID-19 진단을 위한 다양한 샘플링 장치에 의한 사전 분석 샘플 안정화Article Published on 2022-09-252022-09-11 Journal: New biotechnology [Category] COVID19(2023년), MERS, SARS, 진단, 치료기술, [키워드] Bacterial cell culture systems copy number copy numbers COVID-19 Critical detectability detectable diagnostic diagnostic testing diagnostics examined except for gold gold standard growth handling healthcare worker infectious Infectivity inhibited outbreak pandemic Patient Pre-analytic Quantitative RT-PCR reduction remained RNA RNA stability room temperature Saliva Saliva collection devices SARS-CoV-2 SARS-CoV-2 pandemic SARS-CoV-2 RNA Sensitivity and specificity specimen specimen collection stability Swab Swab systems. temperature test results tested the SARS-CoV-2 Transport Viral Viral RNA [DOI] 10.1016/j.nbt.2022.04.001 PMC 바로가기 [Article Type] Article
A qualitative RT-PCR assay for the specific identification of the SARS-CoV-2 B.1.1.529 (Omicron) Variant of ConcernSARS-CoV-2 B.1.1.529(Omicron) 변형 우려의 특정 식별을 위한 정성적 RT-PCR 분석Article Published on 2022-07-012022-09-11 Journal: Journal of Clinical Virology [Category] COVID19(2023년), MERS, SARS, 변종, 진단, 치료기술, [키워드] Alpha Alpha variant Alpha VOC B.1.1.529 B.1.1.7 B.1.351 B.1.429 B.1.526 B.1.617.1 B.1.617.2 Beta can be used circulating clinical sample clinical samples collected Combination concern copy numbers Delta detect develop Epsilon Gamma Genome in silico Iota lineages negative controls non-specific omicron P.1 performed positive replaced REPLACED BY RNA RNA genome RT-PCR RT-PCR assay SARS-CoV-2 Sequencing Specific Specific identification. specificity the SARS-CoV-2 variant virus VoC were used Wuhan [DOI] 10.1016/j.jcv.2022.105191 PMC 바로가기 [Article Type] Article
SARS-CoV-2 wastewater surveillance in Germany: Long-term RT-digital droplet PCR monitoring, suitability of primer/probe combinations and biomarker stability독일의 SARS-CoV-2 폐수 감시: 장기 RT-디지털 액적 PCR 모니터링, 프라이머/프로브 조합의 적합성 및 바이오마커 안정성Case Reports Published on 2022-02-152022-09-11 Journal: Water research [Category] COVID19(2023년), MERS, SARS, 바이오마커, 변종, 치료기술, 치료제, [키워드] approach assays B.1.1.7 Biomarker Biomarker stability Case report circulation city Clinical data Clinical tests Combination Concentration conditions contributing to copy numbers coronavirus coronavirus. correlation COVID-19 case detect determine Digital Digital droplet PCR dissolved oxygen Early detection early stage Epidemiology experiment Germany glycol management methodology N501Y N501Y mutation Nsp3 Nsp9 Occurrence ORF ORF1 ORF1ab oxygen pandemic PCR PCR analysis Polyethylene glycol primer probe proportion reported robust SARS-CoV-2 SARS-CoV-2 RNA Seven shown Spread stability Surveillance target target gene Target genes the SARS-CoV-2 titre Treatment variant variants variants of concern Viral Viral RNA was used wastewater Wastewater-based epidemiology wildtype [DOI] 10.1016/j.watres.2021.117977 PMC 바로가기 [Article Type] Case Reports
Performance and usefulness of a novel automated immunoassay HISCL SARS-CoV-2 Antigen assay kit for the diagnosis of COVID-19COVID-19의 진단을위한 새로운 자동화 면역 분석 HISCL SARS-COV-2 항원 분석 키트의 성능 및 유용성Article Published on 2021-12-012022-09-01 Journal: Scientific Reports [Category] SARS, 진단, 치료기술, 치료법, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Analysis anti-SARS-CoV antibodies anti-SARS-CoV antibody Antigen automated best Chain Reaction chemiluminescence common cold copy numbers coronavirus coronavirus disease Coronavirus disease 2019 CoV antibodies COVID-19 cross-react cut-off detect detection systems diagnosed diagnosis of COVID-19 evaluate help human coronavirus human coronaviruses immunoassay individual Influenza Nasopharyngeal swab nasopharyngeal swab sample Nasopharyngeal swab samples nucleocapsid overdiagnosis pandemic Patient performance polymerase chain polymerase chain reaction positive Protein Proteins Quantitative Rapid reduce respiratory reverse transcription reverse transcription quantitative polymerase chain reaction RT-qPCR SARS-CoV-2 sensitivity severe acute respiratory syndrome Coronavirus tested Virology virus load was determined was tested western blot Western blot analysis [DOI] 10.1038/s41598-021-02636-x PMC 바로가기 [Article Type] Article
Ultrarapid detection of SARS-CoV-2 RNA using a reverse transcription–free exponential amplification reaction, RTF-EXPAR역전사 없는 지수 증폭 반응, RTF-EXPAR을 사용한 SARS-CoV-2 RNA의 초고속 검출403 Published on 2021-08-312022-09-10 Journal: Proceedings of the National Academy of Sciences of [Category] MERS, SARS, 유전자 메커니즘, 진단, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 amplification amplified amplify approach bypassing cleavage copy numbers coronavirus COVID-19 COVID-19 assay DNA equipment EXPAR faster Fluorescence generate genomic RNA infectious agents isothermal isothermal amplification Laboratory Loop-mediated isothermal amplification methodology microliter nucleic acids Pathogens PCR protocol qPCR reported required respiratory responsible reverse transcription RNA RNA detection RT-LAMP RT-qPCR SARS-CoV-2 SARS-CoV-2 RNA selective severe acute respiratory syndrome Coronavirus signal Significance Stage Trigger virus virus genome while [DOI] 10.1073/pnas.2100347118 PMC 바로가기 [Article Type] 403
Development of robust isothermal RNA amplification assay for lab-free testing of RNA viruses실험실이 필요 없는 RNA 바이러스 검사를 위한 강력한 등온 RNA 증폭 분석법 개발Article Published on 2021-08-062022-09-10 Journal: Scientific Reports [Category] MERS, SARS, 진단, 치료기술, [키워드] 16S rRNA Amplification assay applicability biomedical engineering Concentration copy numbers COVID-19 development E. coli cell E. coli cells heat heat-inactivated human saliva infectiousness isothermal Laboratory lateral flow minutes nucleic acid amplification nucleic acids point of care primary focus RNA RNA fragment RNA virus RNA virus robust rRNA Saliva SARS-CoV-2 SARS-CoV-2 virus sensitivity simple Synthetic biology Viral Viral load virus [DOI] 10.1038/s41598-021-95411-x PMC 바로가기 [Article Type] Article
Gamma-irradiated SARS-CoV-2 vaccine candidate, OZG-38.61.3, confers protection from SARS-CoV-2 challenge in human ACEII-transgenic mice감마-조사 된 SARS-COV-2 백신 후보자, OZG-38.61.3은 인간 aceii-transgenic 마우스에서 SARS-COV-2 도전으로부터 보호를 제공한다.Article Published on 2021-08-042022-09-01 Journal: Scientific Reports [Category] MERS, SARS, 변종, 비임상, 유전자 메커니즘, 진단, 치료제, [키워드] added applied approach BALB/c mice breath caused cellular immune responses Challenge test clinical trial copy numbers COVID-19 pandemic dose Efficacy followed by Hepatitis hepatitis A histological analysis Human humoral Humoral and cellular immune responses immune responses immunization immunized immunogenicity inactivated Inactivated Vaccines inactive Infectious diseases lung lung tissues mice Neutralizing antibodies neutralizing antibody Oropharyngeal pandemic Pathogenesis Phase 1 Phase 1 clinical trial Protective purification SARS-CoV-2 SARS-CoV-2 vaccine SARS-CoV-2 viral RNA SARS-CoV-2 virus shown smallpox the SARS-CoV-2 virus the vaccine Toxicity transgenic mice Vaccine vaccine candidate Vaccine development Viral Viral RNA virus [DOI] 10.1038/s41598-021-95086-4 PMC 바로가기 [Article Type] Article
Longitudinal COVID-19 Surveillance and Characterization in the Workplace with Public Health and Diagnostic Endpoints공중 보건 및 진단 엔드포인트가 있는 직장에서의 종단적 COVID-19 감시 및 특성화Research Article Published on 2021-07-072022-09-10 Journal: mSphere [Category] MERS, SARS, 치료기술, [키워드] 95% confidence interval absence acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 analyzed antibodies antibody concentration Asymptomatic Care characterization clinical study Concentration concentrations copy numbers coronavirus coronavirus disease Coronavirus disease 2019 Course COVID-19 COVID-19 infection detectable Disease surveillance Economy effective vaccine example global pandemic half-life Health Health care Hospitalization IgA IgM and IgG immune response Impact individual initiated Intervention Isolation long-term infection longitudinal nasal oral swab pandemic participant PCR positive precipitated predict primary intervention primary interventions productivity Public public health public health measure public health measures quantitative PCR reduce remained respiratory resulting RT-qPCR Safe SARS-CoV-2 SARS-CoV-2 RNA SARS-CoV-2 transmission serology serum severe acute respiratory syndrome Coronavirus Spread study participant Study participants subject Surveillance Therapeutics Transmission vaccination rate Viral Viral RNA was used widespread Workplace [DOI] 10.1128/mSphere.00542-21 PMC 바로가기 [Article Type] Research Article
The detection and stability of the SARS-CoV-2 RNA biomarkers in wastewater influent in Helsinki, Finland핀란드 헬싱키의 폐수 유입수에서 SARS-CoV-2 RNA 바이오마커의 검출 및 안정성Article Published on 2021-05-202022-09-11 Journal: The Science of the total environment [Category] MERS, SARS, 바이오마커, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addition Analysis analyzed Betacoronavirus Biomarker collected conditions copy numbers coronavirus COVID-19 disease decay Decay characteristics fraction Frequency Helsinki IMPROVE influent linear lockdown lockdowns many cases MONITOR Municipal sewage New New coronavirus Norovirus Particulate matter persistence Prevalence quantified quantitative PCR remained respiratory RNA RNA copy number RNA copy numbers RT-qPCR SARS-CoV-2 SARS-CoV-2 RNA SARS-CoV-2 virus severe acute respiratory syndrome Coronavirus stability statistically significant storage storage condition storage conditions Storage conditions. supernatant Surveillance temperature the SARS-CoV-2 the SARS-CoV-2 virus Treatment Ultrafiltration was tested wastewater water [DOI] 10.1016/j.scitotenv.2021.145274 PMC 바로가기 [Article Type] Article
Persistence of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Virus and Viral RNA in Relation to Surface Type and Contamination Concentration표면 유형 및 오염 농도와 관련된 중증 급성 호흡기 증후군 코로나바이러스 2(SARS-CoV-2) 바이러스 및 바이러스 RNA의 지속성Public and Environmental Health Microbiology Published on 2021-05-072022-09-10 Journal: Applied and Environmental Microbiology [Category] MERS, SARS, 치료기술, [키워드] Analysis Concentration Contact contact with contaminated surfaces Contamination copy number copy numbers coronavirus 2 COVID-19 Cycle threshold values detect determine environmental persistence environmental sampling exhibited Expected level expected levels Genome high stability hydrophobic hydrophobic surfaces implication independent independent of Interpretation lead occur polyester quantity reduced reduction reduction in relation respiratory reverse transcription reverse transcription-PCR RNA RNA copies RNA copy RNA copy number RNA copy numbers RT-PCR SARS-CoV-2 SARS-CoV-2 RNA stainless steel surface surface sampling surgical Transmission transmission of SARS-CoV-2 Type undetectable viability Viable virus was reduced [DOI] 10.1128/AEM.00526-21 PMC 바로가기 [Article Type] Public and Environmental Health Microbiology