SARS-CoV-2 Spike Protein Mutation at Cysteine-488 Impairs Its Golgi Localization and Intracellular S1/S2 ProcessingArticle Published on 2022-12-132023-07-11 Journal: International Journal of Molecular Sciences [Category] COVID19(2023년), [키워드] Brefeldin A cysteine proteolysis SARS-CoV-2 Spike. [DOI] 10.3390/ijms232415834 PMC 바로가기
Novel Regioselective Approach to Cyclize Phage-Displayed Peptides in Combination with Epitope-Directed Selection to Identify a Potent Neutralizing Macrocyclic Peptide for SARS-CoV-2Article Published on 2022-10-212022-11-15 Journal: ACS chemical biology [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] ACE2 acute respiratory syndrome angiotensin-converting enzyme 2 antivirals Combination cysteine cysteines effective epitope human host inhibit Interaction iterative methodology N-terminal novel peptide peptides potent Protein RBD reaction receptor Receptor binding domain SARS-CoV-2 Selection spike the SARS-CoV-2 the Spike Vero E6 cell virus were used [DOI] 10.1021/acschembio.2c00565 PMC 바로가기
Targeting SARS-CoV-2 Main Protease for Treatment of COVID-19: Covalent Inhibitors Structure-Activity Relationship Insights and Evolution PerspectivesArticle Published on 2022-10-132022-11-15 Journal: Journal of medicinal chemistry [Category] COVID19(2023년), SARS, 치료제, [키워드] analysis analyzed antiviral drug catalytic category cysteine eight electrophilic enzyme example highlighting in silico in vitro in vivo inhibitor inhibitors insight involved mechanism Pathways protease protocols provided reactive relationship Replication reported SARS-CoV-2 Scientific community selective target targeting the SARS-CoV-2 virus without affecting [DOI] 10.1021/acs.jmedchem.2c01005 PMC 바로가기
A systematic exploration of boceprevir-based main protease inhibitors as SARS-CoV-2 antiviralsSARS-CoV-2 항바이러스제로서의 보세프레비르 기반 주요 프로테아제 억제제의 체계적인 탐색Article Published on 2022-10-052022-09-11 Journal: European journal of medicinal chemistry [Category] COVID19(2023년), SARS, 변종, 치료제, [키워드] 293T cell 293T cells active site Alanine aldehyde amide Antiviral antiviral potency boceprevir Carbamate carbamide changed characterized complex covalent inhibitor COVID-19 crystal structure crystal structures cysteine cytotoxicity expressed HCV hydrophobic hydrophobic interaction IMPROVE in vitro inhibit inhibitor inhibitors isovaleramide M pro main protease Modification modifications N-terminal nitrile observation Opal PF-07321332 Pro protease Protease inhibitor recognize residue reversed SARS-CoV-2 SARS-CoV-2 main protease SARS-CoV-2 variants SARS-CoV-2. side chain substrate tested the SARS-CoV-2 undetectable unique warhead [DOI] 10.1016/j.ejmech.2022.114596 PMC 바로가기 [Article Type] Article
A multi-pronged evaluation of aldehyde-based tripeptidyl main protease inhibitors as SARS-CoV-2 antiviralsSARS-CoV-2 항바이러스제로서의 알데히드 기반 트리펩티딜 주요 프로테아제 억제제의 다각적 평가Article Published on 2022-10-052022-09-11 Journal: European journal of medicinal chemistry [Category] COVID19(2023년), SARS, 치료제, [키워드] 293T cell 293T cells active site aldehyde Amino acid amino acids Antiviral antiviral potency binding carbon cellular complexes conducted covalent inhibitors COVID-19 crystal structure cysteine cytotoxicity develop enzyme exhibited expressed flexible high potency in vitro inhibit inhibitor inhibitors interact low potency M pro main protease N-terminal pathogen Previous studies previous study Pro protease Protease inhibitor residue residues SARS-CoV-2 selected Structural variation structures tolerable Toxicity Treatment treatment of COVID-19 Tripeptides. unnatural Variation X-ray [DOI] 10.1016/j.ejmech.2022.114570 PMC 바로가기 [Article Type] Article
A paper-based optical sensor for the screening of viruses through the cysteine residues of their surface proteins: A proof of concept on the detection of coronavirus infection표면 단백질의 시스테인 잔기를 통한 바이러스 스크리닝을 위한 종이 기반 광학 센서: 코로나바이러스 감염 감지에 대한 개념 증명Article Published on 2022-10-012022-09-11 Journal: Talanta [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus BENZOTHIAZOLE Biothiol cause Chain Reaction clinical study Colorimetric assay complex Concentration coronavirus Coronavirus infection current cysteine cysteine residue Disulfide disulfide bond Filter Paper group Health Human immunodeficiency virus human Papilloma virus immunodeficiency intensity lab lack limit of detection linear relationship logarithm lowest naked eye nasopharyngeal swabs optical pandemic paper Paper based sensor papilloma pathology Patient pink color polymerase chain polymerase chain reaction proof proof of concept Protein Proteins qRT-PCR Rapid reduced required reverse transcription reverse transcription polymerase chain reaction S1 protein Saliva SARS-CoV-2 SARS-CoV-2. screening method severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 specimens Spike protein subunit sulfhydryl the S1 protein time-consuming virus virus viruses [DOI] 10.1016/j.talanta.2022.123630 PMC 바로가기 [Article Type] Article
FASN inhibitor TVB-3166 prevents S-acylation of the spike protein of human coronavirusesArticle Published on 2022-09-012022-11-15 Journal: Journal of Lipid Research [Category] COVID19(2023년), SARS, 진단, 치료기술, [키워드] acute respiratory syndrome Cell click chemistry conserved coronavirus CoV CoV-229E Critical cysteine de novo enzyme epithelial cells examined expressed FASN fatty Fatty acid host cell Host cell entry human coronavirus in vitro incubated Infection inhibited inhibitor isoform Modification modify Multiple other coronavirus palmitate phylogenetically Prevent Protein reduction in reported residues S-acylation SARS-CoV-2 specificity spike coronavirus. Spike protein Spread the spike protein ZDHHC ZDHHC5 [DOI] 10.1016/j.jlr.2022.100256 PMC 바로가기
Towards Label-free detection of viral disease agents through their cell surface proteins: Rapid screening SARS-CoV-2 in biological specimensArticle Published on 2022-09-012022-11-15 Journal: SLAS discovery : advancing life sciences R & D [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] approach biological fluid Blood blood plasma Care Cell clinical settings country current cysteine cysteine residue disulfide bond ELISA enzyme-linked immunosorbent group Human Human blood plasma Human immunodeficiency virus Label-free SERS lack medium POC polymerase chain Protein Proteins purified Raman spectroscopy Rapid required residues reverse transcription RT-qPCR S1 protein Saliva SARS-CoV-2 SARS-CoV-2 virus specimen surface proteins the S1 protein thiol Thiol chemistry time-consuming viral capsid Viral capsid proteins. viral disease viral infection virus virus viruses was used [DOI] 10.1016/j.slasd.2022.06.001 PMC 바로가기
Surface cysteines could protect the SARS-CoV-2 main protease from oxidative damageArticle Published on 2022-09-012023-07-09 Journal: Journal of inorganic biochemistry [Category] COVID19(2023년), [키워드] cysteine reactive oxygen species SARS-CoV-2 main protease. [DOI] 10.1016/j.jinorgbio.2022.111886 PMC 바로가기
Papain-like protease of SARS-CoV-2 inhibits RLR signaling in a deubiquitination-dependent and deubiquitination-independent mannerArticle Published on 2022-08-122022-10-06 Journal: Frontiers in Immunology [Category] SARS, 진단, [키워드] acute respiratory syndrome antiviral therapy attenuate catalytic component components contribute Coronavirus-2 COVID-19 Critical cysteine demonstrated Deubiquitination disease severity domain DUB dysregulated Effect Efficiency exhibited greater IFN IFN response independent inhibit inhibitory activity inhibitory effect innate immune interferon IRF3 IκBα MAVS mechanism mechanisms mutant Papain-like protease papain-like protease. PLPro protease receptor reduced reported required residue response responses RIG-I RLR signaling SARS-CoV SARS-CoV-2 SARS-CoV-2 pathogenesis Sendai virus Signal transduction Signaling signaling pathway significantly specificity suppressed target TBK1 TRAF3 Traf6 [DOI] 10.3389/fimmu.2022.947272 PMC 바로가기 [Article Type] Article