Potential pitfalls in analysing a SARS-CoV-2 RT-PCR assay and how to standardise data interpretationSARS-CoV-2 RT-PCR 분석 및 데이터 해석을 표준화하는 방법 분석의 잠재적 함정Article Published on 2022-10-012022-09-11 Journal: Journal of virological methods [Category] COVID19(2023년), SARS, 치료기술, [키워드] addition analysed Applied Biosystem Audit automated baseline Baseline threshold carried complex data interpretation determine Diagnostics.ai Frequency implementation in some increase in Interpretation introduced investigated Laboratory manual intervention Patient PCR positive result Potential processed produced provide Real-time PCR real-time RT-PCR relative Relative threshold RT-PCR RT-PCR kit RT-PCR kits SARS-CoV-2 SARS-CoV-2 RT-PCR solution tested thermocyclers threshold time-consuming virus detection was determined [DOI] 10.1016/j.jviromet.2022.114589 PMC 바로가기 [Article Type] Article
Minimizing errors in RT-PCR detection and quantification of SARS-CoV-2 RNA for wastewater surveillance폐수 감시를 위한 SARS-CoV-2 RNA의 RT-PCR 검출 및 정량화 오류 최소화Review Published on 2022-01-202022-09-11 Journal: The Science of the total environment [Category] SARS, 치료기술, [키워드] analyses approaches Chain Reaction communicable diseases Community comparison Concentration controls coronavirus disease Coronavirus disease-19 COVID-19 COVID-19 infection COVID-19 infections COVID-19 pandemic Critical data interpretation demonstrated effective Efficacy Factor false False negative False positive false-negative False-positive health information identify IMPROVE incidence include Inclusion Inconclusive information initial interpretability Laboratory Measures mitigate pathogen Pathogens PCR polymerase chain reaction positive public health Quality assurance Quality control quantification reliability respiratory retrospective reverse transcription Reverse transcription-polymerase chain reaction RNA extraction RT-PCR RT-PCR assay RT-PCR detection SARS-CoV-2 SARS-CoV-2 RNA Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus-2 silver standardized protocol standardized protocols Surveillance virus wastewater [DOI] 10.1016/j.scitotenv.2021.149877 PMC 바로가기 [Article Type] Review
Transcriptional Profiling and Machine Learning Unveil a Concordant Biosignature of Type I Interferon-Inducible Host Response Across Nasal Swab and Pulmonary Tissue for COVID-19 Diagnosis전사 프로파일링 및 기계 학습, COVID-19 진단을 위한 비강 면봉 및 폐 조직 전반에 걸쳐 I형 인터페론 유도성 숙주 반응의 일치하는 생체특징 공개Immunology Published on 2021-11-222022-09-11 Journal: Frontiers in Immunology [Category] MERS, SARS, 유전자 메커니즘, 진단, 치료기술, [키워드] adjusted adopted Analysis approach biosignature BST2 caused complementary computational analysis concordant conserved COVID-19 COVID-19 diagnosis data interpretation dataset Diagnosis Diagnostic method embedding Factor false-negative Genes geometric global pandemic GSE147507 heterogeneous high mortality homological Host host response IFIT1 IFIT2 IFIT3 IFITM1 IFN-I IFN-I signaling infection control Interferome interferon involved ISG15 Limited lung tissue machine learning morbidity MX1 MX2 nasal swab NCBI-GEO network OAS1 OAS2 OAS3 OASL objective other respiratory infections outcome Profiling pulmonary respiratory respiratory infections response Result retrieved RNA-seq data RNA-sequencing RSAD2 RT-PCR SARS-CoV-2 SARS-CoV-2 virus sequencing data severity of COVID-19 similarity Stat1 stochastic stratified transcriptional transcriptional level transcriptomics Type type I interferon virus was used WGCNA [DOI] 10.3389/fimmu.2021.733171 PMC 바로가기 [Article Type] Immunology
Explorative assessment of coronavirus-like short sequences from host-associated and environmental metagenomes숙주 관련 및 환경 메타게놈에서 코로나바이러스와 유사한 짧은 서열의 탐색적 평가Article Published on 2021-11-012022-09-11 Journal: The Science of the total environment [Category] SARS, 유전자 메커니즘, 치료기술, [키워드] aggravate Analysis analyzed approaches caused Coronaviruses COVID-19 COVID-19 pandemic data interpretation dataset datasets Deepening DNA sequence expand healthcare Host indicated knowledge metagenome metagenomic metagenomics Natural environment nucleotide nucleotide sequence parameter Previous studies previous study SARS-CoV-2 selected sequence situation terrestrial Virome profiling. virus detection [DOI] 10.1016/j.scitotenv.2021.148494 PMC 바로가기 [Article Type] Article
COVID19 Disease Map, a computational knowledge repository of virus–host interaction mechanisms코로나19 질병 지도, 바이러스-숙주 상호작용 메커니즘의 전산 지식 저장소Article Published on 2021-10-192022-09-10 Journal: Molecular Systems Biology [Category] MERS, SARS, 유전자 메커니즘, 치료제, [키워드] affected Analysis approach build Community community effort complex computable knowledge repository Computational biology COVID‐19 COVID19 curated data interpretation dataset disease domain drug candidates example framework help highlight hypotheses Interaction Intervention involved knowledge large‐scale biocuration Map mechanism Microbiology, Virology & Host Pathogen Interaction molecular mechanism molecular mechanisms network analysis omics data analysis Open Access open access community effort Pandemics pathway Pathways Perspective platform predisposition Repository resource SARS‐CoV‐2 SARS‐CoV‐2 infection Support systems biomedicine target text mining Treatment response virus [DOI] 10.15252/msb.202110387 PMC 바로가기 [Article Type] Article
Potential Use of Untreated Wastewater for Assessing COVID-19 Trends in Southern ItalyArticle Published on 2021-09-292022-10-29 Journal: International Journal of Environmental Research an [Category] COVID-19, SARS, [키워드] affecting applied Assessing can be used characteristic Characteristics clinical disease collected complement Concentration coronavirus coronavirus 2 coronavirus disease COVID-19 data interpretation Epidemics Epidemiology Genome IMPROVE include investigated Italy knowledge molecular analysis occur PCR plant positive positive sample Potential predict Prevalence quantitative PCR ranged respiratory RNA ROC SARS-CoV-2 sensitivity specificity Surveillance surveillance data Switzerland Treatment trend variable virus wastewater Wastewater-based epidemiology WHO World Health Organization [DOI] 10.3390/ijerph181910278 PMC 바로가기 [Article Type] Article
Lives saved with vaccination for 10 pathogens across 112 countries in a pre-COVID-19 worldResearch Article Published on 2021-07-132022-10-31 Journal: eLife [Category] COVID-19, SARS, [키워드] activity Alliance ART authors BAL Cambridge center children Clinical research country Coverage Dame Data analysis data interpretation death Department development disease Diseases effective Fever Final foundation funding group had no Haemophilus influenzae hepatitis B human papillomavirus Impact Imperial College London Japanese encephalitis Johns Hopkins University LMICs London mathematical Mathematical modelling Medical Research Council Medicine Melinda Gate MRC Neisseria meningitidis occur Other Oxford pathogen Pennsylvania State University provide Public Health England Public health interventions receive Richard School Science Singapore Streptococcus pneumoniae Study design Support supported Travel unit university vaccination Vaccine Vaccine impact virus was used Washington [DOI] 10.7554/eLife.67635 PMC 바로가기 [Article Type] Research Article
Long-Term Longitudinal Evaluation of Six Commercial Immunoassays for the Detection of IgM and IgG Antibodies against SARS CoV-2SARS COV-2에 대한 IgM 및 IgG 항체의 검출에 대한 6 개의 상업적 면역 분석의 장기 종단 평가Multicenter Study Published on 2021-06-262022-09-01 Journal: Viruses [Category] MERS, SARS, 진단, 치료기술, 치료제, [키워드] Analysis antibody antibody levels Antigen assays best Characteristics Chemiluminescent assay chemiluminescent immunoassay CLIA clinical form CMIA concern COVID-19 data interpretation detection disease severity ECLIA ELISA evaluated Follow-up visit follow-up visits healthy humoral Humoral response IgG IgM IgM and IgG immunoassay Increases independent longitudinal Microparticle moderate Patient performed positive post-symptom onset PSO raised remained required sample selection SARS CoV-2 SARS-CoV-2 SARS-COV-2 infection sensitivity Seroconversion Serological assay serological assays serology Seropositivity serum sample serum samples severity Severity of disease significantly specificity the disease were used [DOI] 10.3390/v13071244 PMC 바로가기 [Article Type] Multicenter Study
Maintenance of neutralizing antibodies over ten months in convalescent SARS‐CoV‐2 afflicted patients회복기 SARS-CoV-2 환자에서 중화항체를 10개월 이상 유지Original Article Published on 2021-05-272022-09-01 Journal: Transboundary and emerging diseases [Category] COVID19(2023년), MERS, SARS, 진단, [키워드] analysed Antibody titre approaches chemiluminescent immunoassay convalescent convalescent individual convalescent individuals data interpretation develop Evidence finding humoral immune response IgG antibodies immune responses Immunity immunofluorescence assay individual Infection investigated lack maintain maintenance Neutralization assay neutralization test Neutralizing antibodies neutralizing antibody Patient persistence positive correlation Primary infection Protective protective immunity SARS‐CoV‐2 SARS‐CoV‐2 Severity of infection specific antibodies [DOI] 10.1111/tbed.14130 PMC 바로가기 [Article Type] Original Article
Risk factors for poor outcomes in hospitalised COVID-19 patients: A systematic review and meta-analysisResearch Theme 6: UNCOVER - Usher Institute Network of COVID-19 Evidence Reviews Published on 2021-03-012022-10-31 Journal: Journal of Global Health [Category] COVID-19, SARS, [키워드] 95% confidence interval absence adjusted Admission adopted age Cancer Characteristics Chronic kidney disease chronic obstructive clinical factor COVID-19 COVID-19 patient data interpretation database diabete effect size Evidence Factor Factors help hospitalisation hospitalised hospitalised COVID-19 patients ICU identify increased risk indicated intensive care Interpretation Invasive mechanical ventilation Joanna Briggs Institute Male sex meta-analyses Meta-analysis Mortality MOST obesity Odds ratio Older age outcome outcomes Population provide pulmonary disease reported required Research Result risk risk factor searched Sex smoker synthesised systematic review the WHO treatment for COVID-19 understanding were assessed [DOI] 10.7189/jogh.11.10001 PMC 바로가기 [Article Type] Research Theme 6: UNCOVER - Usher Institute Network of COVID-19 Evidence Reviews