differential scanning fluorimetry – Communicable Diseases Research

A thermostable Cas12b from Brevibacillus leverages one-pot discrimination of SARS-CoV-2 variants of concern

Brevibacillus의 열안정성 Cas12b는 우려되는 SARS-CoV-2 변이체의 원팟 식별을 활용합니다.

Article

Published on 2022-03-012022-09-11
Journal: EBioMedicine [Category] SARS, 변종, 치료기술,

[키워드] Accuracy activity Alicyclobacillus Alpha amplification applied B.1.1.529 B.1.1.7 B.1.351 B.1.617.2 Beta Breast Breast cancer Cas12b center clinical samples college Complete Control COVID-19 CRISPR Ct value current Delta detection system diagnostics differential scanning fluorimetry disease control dissemination Dual-detection Engineering facilitate foundation fraction Gamma guide RNA high viral load house implementation isothermal amplification lack landscape Loop-mediated isothermal amplification Medicine Negative predictive value NIAID offer office omicron One-pot detection overcome P.1 Palindromic Pathogens platform point-of-care positive Predictive value President prevention primer Protein purified qPCR reaction reagent Real-time monitoring reverse transcription robust RT-LAMP S gene SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2 variant Science sensitivity Sequencing SPADE specificity technology the SARS-CoV-2 Thermophillic university variants variants of concern veterinary medicine vice Vice President Viral load VoC VOCs were expressed
[DOI] 10.1016/j.ebiom.2022.103926 PMC 바로가기 [Article Type] Article

D614G mutation in the SARS-CoV-2 spike protein enhances viral fitness by desensitizing it to temperature-dependent denaturation

Accelerated Communication

Published on 2021-09-242022-10-30
Journal: The Journal of Biological Chemistry [Category] COVID-19, MERS, SARS,

[키워드] 3DVA, three-dimensional variability analysis ACE2, angiotensin converting enzyme 2 Alter approach binding Characteristics cold-induced unfolding conformational conjugate COVID-19 pandemic Cryo-electron microscopy CTF, contrast transfer function D614G D614G mutation demonstrated denaturation differential scanning fluorimetry dominant DSC, differential scanning calorimetry DSF, differential scanning fluorimetry ENhance exceptional exhibited host receptor mechanism Microscopy molecular basis Multiple Mutation NSEM, negative staining electron microscopy analysis physiological protein stability RBD, receptor-binding domain RBDs RBM, receptor-binding motif Receptor-binding domain SARS-CoV-2 SARS-CoV-2, Severe acute respiratory syndrome coronavirus 2 significantly Spike protein stability staining the SARS-CoV-2 the spike protein thermal stability Thermodynamics Vaccine development variant viral fitness Viral protein virus WT, wild type
[DOI] 10.1016/j.jbc.2021.101238 PMC 바로가기 [Article Type] Accelerated Communication

Fragment binding to the Nsp3 macrodomain of SARS-CoV-2 identified through crystallographic screening and computational docking

결정학적 스크리닝 및 컴퓨터 도킹을 통해 확인된 SARS-CoV-2의 Nsp3 매크로도메인에 대한 단편 결합

Research Article

Published on 2021-04-142022-08-13
Journal: Science Advances [Category] Biochemistry, COVID-19, SARS,

[키워드] active site acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 adenosine adenosine diphosphate Antiviral Antiviral agents antiviral target binding catalytic chemotype chemotypes conformational coronavirus counteract Data collection differential scanning fluorimetry docking effort enzyme Fluorescence foundation fragment heterogeneity homogeneous host-mediated antiviral adenosine diphosphate–ribosylation signaling inhibitor inhibitors isothermal titration calorimetry macrodomain macrodomain-binders massive Mutation nonpathogenic nonstructural protein 3 physiological temperature protein 3 provide respiratory SARS-CoV-2 selected severe acute respiratory syndrome Coronavirus Signaling starting point starting points Structure unique virus viruses X-ray data collection
[DOI] 10.1126/sciadv.abf8711 PMC 바로가기 [Article Type] Research Article

High-resolution structure and biophysical characterization of the nucleocapsid phosphoprotein dimerization domain from the Covid-19 severe acute respiratory syndrome coronavirus 2

Covid-19 중증급성호흡기증후군 코로나바이러스 2의 뉴클레오캡시드 인단백질 이량체화 도메인의 고해상도 구조 및 생물물리학적 특성 규명

Article

Published on 2021-01-292022-09-11
Journal: Biochemical and Biophysical Research Communication [Category] SARS, 치료법,

[키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 antiviral target binding C-terminal domain caused complex conformational change coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 Cryo-electron microscopy crystal structure crystal structures CTD demonstrated develop differential scanning fluorimetry domain electron microscopy full-length function genomic Health Health crisis immunogenic immunogenic determinant initial moderate multifunctional nucleocapsid Nucleocapsid phosphoprotein oligomerization pandemic pathogenicity Protein provide respiratory RNA RNA binding SARS Coronavirus SARS-CoV N SARS-CoV-2 SARS-CoV-2 N scattering severe acute respiratory syndrome Coronavirus single-stranded solution ssRNA tendency the SARS-CoV-2 thermal stability ultracentrifugation Viral viral genome virulence
[DOI] 10.1016/j.bbrc.2020.09.131 PMC 바로가기 [Article Type] Article

Discovery of Drug-Like Ligands for the Mac1 Domain of SARS-CoV-2 Nsp3

Article

Published on 2020-09-282022-10-29
Journal: SLAS discovery : advancing life sciences R & D [Category] COVID-19, SARS,

Biophysical comparison of diphtheria and tetanus toxins with the formaldehyde-detoxified toxoids, the main components of diphtheria and tetanus vaccines

디프테리아 및 파상풍 독소와 포름알데히드 해독된 독소의 생물물리학적 비교, 디프테리아 및 파상풍 백신의 주요 성분

Article

Published on 2017-11-172024-08-18
Journal: Virulence [Category] 파상풍,

[키워드] Circular dichroism differential scanning fluorimetry Diphtheria MALDI-TOF mass spectrometry small-angle X-ray scattering Tetanus toxin toxoid Vaccine