Validation of improved automated nucleic acid extraction methods for direct detection of polioviruses for global polio eradicationarticle Published on 2024-05-312024-09-01 Journal: Journal of Virological Methods [Category] 폴리오, [키워드] Automated NA extraction Direct detection poliovirus eradication Real-time PCR [Article Type] article
Detection of Enterovirus D68 in Wastewater Samples from the UK between July and November 2021Brief Report Published on 2022-01-132022-10-28 Journal: Viruses [Category] Coronavirus, [키워드] Active surveillance acute flaccid myelitis (AFM) AFM approach association circulating circulation clade clinical sample clinically COVID-19 pandemic detect detection Direct detection enterovirus Enterovirus D68 Environmental surveillance European expected Genetic human enterovirus D68 increase in Infection introduced Neurological disease Next-generation sequencing next-generation sequencing (NGS) NGS nonpharmaceutical intervention Prevalence reducing reported Sample sequence serotype strain Surveillance Transmission dynamics United Kingdom utility variants virus viruses wastewater [DOI] 10.3390/v14010143 PMC 바로가기 [Article Type] Brief Report
Culture-Independent Detection of Poliovirus in Stool Samples by Direct RNA Extraction문화 독립적인 폴리오바이러스의 대변 샘플에서 직접 RNA 추출에 의한 검출Article Published on 2021-12-222024-08-26 Journal: Microbiol Spectr [Category] 폴리오, [키워드] culture-independent diagnostics Direct detection Polio Eradication Poliovirus RNA extraction rRT-PCR
Rapid Increase of SARS-CoV-2 Variant B.1.1.7 Detected in Sewage Samples from England between October 2020 and January 2021Research Article Published on 2021-06-152022-10-29 Journal: mSystems [Category] COVID-19, [키워드] affecting Amino acid Amplicon antigenicity B.1.1.7 B.1.351 Brazil can be used Clinical data collected COVID-19 COVID-19 pandemic Critical detect Direct detection E484K Efficacy England Environmental surveillance Frequency growth help human immune response in viral increase increase in independent investigated Lineage B.1.1.7 London Mutation new SARS-CoV-2 Next-generation sequencing P.1 prevalent quantification raise raising Rapid RNA Sample SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 variant Sequencing analysis sewage shown South Africa spike mutation Surveillance the spike protein Transmission Vaccine variant variant B.1.1.7 variant of concern variants of concern virus virus transmission viruses VoC VOCs wastewater widespread [DOI] 10.1128/mSystems.00353-21 PMC 바로가기 [Article Type] Research Article
Comparison of SARS-CoV-2 indirect and direct RT-qPCR detection methodsSARS-CoV-2 간접 및 직접 RT-qPCR 검출 방법 비교Comparative Study Published on 2021-05-172022-09-01 Journal: Virology Journal [Category] SARS, 진단, 치료기술, [키워드] 2019-nCoV Analysis approach best BGI boiling caused Clinical diagnosis comparable Cost analysis COVID-19 COVID-19 pandemic Critical Ct values Detection limit detection system diagnostic diagnostics Direct detection evaluated exhibited extracted RNA gold gold standard handling initial Invitrogen isolation procedures less lysis buffer methodology MOST NEB other treatment Patient performed Primers provided purified viral RNA Qiagen reagents reduced Result RNA extraction RNA extraction kit RNA isolation RNase RNase inhibitor RT-qPCR SARS-CoV-2 SARS-CoV-2 virus sensitive sensitivity specificity supplementary material SYBR Green TaqMan tested the SARS-CoV-2 virus Universal Viral viral genome Viral RNA virus [DOI] 10.1186/s12985-021-01574-4 PMC 바로가기 [Article Type] Comparative Study
Preliminary optimisation of a simplified sample preparation method to permit direct detection of SARS-CoV-2 within saliva samples using reverse-transcription loop-mediated isothermal amplification (RT-LAMP)Article Published on 2021-03-012023-06-19 Journal: Journal of virological methods [Category] MERS, SARS, [키워드] COVID-19 Direct detection Near patient testing rapid diagnostics RT-LAMP Saliva SARS-CoV-2 [DOI] 10.1016/j.jviromet.2020.114048 PMC 바로가기
Specific detection of Trichophyton rubrum and Trichophyton interdigitale based on loop-mediated isothermal amplification (LAMP) from onychomycosis specimensArticle Published on 2019-12-312024-08-30 Journal: The Journal of Dermatology [Category] 백선증, [키워드] culture method dermatophytes Direct detection LAMP method Molecular diagnosis PMC 바로가기
Detection by Direct Next Generation Sequencing Analysis of Emerging Enterovirus D68 and C109 Strains in an Environmental Sample From ScotlandOriginal Research Published on 2018-08-212023-08-30 Journal: Frontiers in Microbiology [Category] E형 간염, [키워드] Direct detection enterovirus pathogenesis Environmental surveillance EV-C109 EV-D68 human enterovirus next generation sequencing [DOI] 10.3389/fmicb.2018.01956 PMC 바로가기 [Article Type] Original Research