Production of Reverse Transcriptase and DNA Polymerase in Bacterial Expression SystemsArticle Published on 2024-07-182024-09-05 Journal: Bioengineering [Category] update2024, [키워드] DNA polymerase Escherichia coli extracellular production protein production reverse transcriptase Vibrio natriegens [DOI] 10.3390/bioengineering11070727 PMC 바로가기 [Article Type] Article
Advancements and applications of loop-mediated isothermal amplification technology: a comprehensive overviewMicrobiology Review Published on 2024-07-172024-09-05 Journal: Frontiers in Microbiology [Category] update2024, [키워드] DNA polymerase Loop-mediated isothermal amplification Molecular diagnosis monitoring methods primer design technical improvement [DOI] 10.3389/fmicb.2024.1406632 PMC 바로가기 [Article Type] Microbiology Review
DNA Polymerase I Large Fragment from Deinococcus radiodurans , a Candidate for a Cutting-Edge Room-Temperature LAMPArticle Published on 2024-01-232024-09-05 Journal: International Journal of Molecular Sciences [Category] update2024, [키워드] Deinococcus radiodurans DNA polymerase DNA replication extremophiles LAMP [DOI] 10.3390/ijms25031392 PMC 바로가기 [Article Type] Article
Analysis of antiviral drug properties of thymidine kinase of herpes B virus using recombinant herpes simplex virus 1Research Article Published on 2024-01-142024-09-04 Journal: Microbiology Spectrum [Category] 대상포진, [키워드] acyclovir acyclovir resistant B virus DNA polymerase Ganciclovir ganciclovir resistant Herpes Simplex Virus 1 thymidine kinases [DOI] 10.1128/spectrum.03091-23 PMC 바로가기 [Article Type] Research Article
Reverse transcriptase-free detection of viral RNA using Hemo Klentaq DNA polymeraseArticle Published on 2022-12-152022-11-15 Journal: Analytical biochemistry [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] affected amplification Analysis can be used caused comparable COVID-19 Degraded RNA demonstrated determine DNA polymerase Efficiency freeze-thaw cycles limitations Nasopharyngeal swab Nearby Nearby primers overcome pandemic patients PCR polymerase chain polymerase chain reaction Primers provide provided reliability reverse transcriptase Reverse transcriptase activity reverse transcription RNA RT-PCR SARS-CoV-2 SARS-CoV-2 detection target transcriptase Viral RNA [DOI] 10.1016/j.ab.2022.114960 PMC 바로가기
Discovery of small molecule inhibitors of Plasmodium falciparum apicoplast DNA polymerase플라스모디움 팔시파룸 아피코플라스트 DNA 중합효소의 소분자 억제제 발견Article Published on 2022-05-052024-08-01 Journal: Journal of Enzyme Inhibition and Medicinal Chemistry [Category] 말라리아, [키워드] Apicoplast DNA polymerase High-throughput screening malaria [DOI] 10.1080/14756366.2022.2070909 PMC 바로가기
Molnupiravir; molecular and functional descriptors of mitochondrial safety몰누피라비르; 미토콘드리아 안전성의 분자 및 기능 설명자Article Published on 2022-05-012022-09-11 Journal: Toxicology and applied pharmacology [Category] COVID19(2023년), SARS, 유전자 메커니즘, 치료제, [키워드] active form Adult patients Analyzer antiviral drug antiviral drugs approved arrest cell line Concentration concentrations copy number COVID-19 Culture cytotoxicity DNA polymerase dose drug emergency treatment Endpoint expression flux functional Genome HepG2 HepG2 cell incorporation inhibit lack mechanism metabolite Mitochondria mitochondrial mitochondrial DNA Mitochondrial function mitochondrial gene molecular molnupiravir Mutation N-hydroxycytidine NHC nucleoside Patient phenotypic PolG POLRMT progression RdRP replicating virus Replication resulting risk RNA polymerase significantly specificity the SARS-CoV-2 Toxicity Transcription Treatment triphosphate U.S. FDA viral genome virus was measured [DOI] 10.1016/j.taap.2022.116003 PMC 바로가기 [Article Type] Article
FV-100 for the Treatment of Varicella-Virus (VZV) Infections: Quo Vadis?FV-100을 수두 바이러스(VZV) 감염 치료에 대한: 어디로 가는가?Review Published on 2022-04-072023-05-31 Journal: Viruses [Category] 대상포진, 두창, 수두, 홍역, [키워드] Cf1743 DNA polymerase FV-100 herpes zoster thymidine kinase Varicella-Zoster Virus [DOI] 10.3390/v14040770 PMC 바로가기 [Article Type] Review
Multiplex, Real-Time, Point-of-care RT-LAMP for SARS-CoV-2 Detection Using the HFman ProbeHFman 프로브를 사용한 SARS-CoV-2 검출을 위한 다중, 실시간, 현장 진료 RT-LAMPArticle Published on 2022-03-252022-09-11 Journal: ACS Sensors [Category] COVID19(2023년), SARS, 변종, 유전자 메커니즘, 치료기술, [키워드] addition affecting Alpha alpha and delta variants amplification arising binding Chain Reaction clinical sample COVID-19/SARS-CoV-2 Delta variants detection diagnostic diagnostic test DNA DNA polymerase Efficiency evade Evolution extracted RNA gold gold standard HFman probe high-fidelity DNA polymerase Impact isothermal LAMP limitation mechanism multiplex multiplex LAMP nasopharyngeal sample Nasopharyngeal samples nasopharyngeal swabs non-specific non-specific amplification nucleic acid overcome performed POCT point-of-care point-of-care testing (POCT). polymerase chain polymerase chain reaction positive primer probe Quantitative reaction Result reverse transcription RT-LAMP SARS-CoV-2 SARS-CoV-2 viral selective sensitivity sequence sequences specificity Spread subject Tolerance variant variants viral evolution [DOI] 10.1021/acssensors.1c02079 PMC 바로가기 [Article Type] Article
Polyphenols as Potential Inhibitors of SARS-CoV-2 RNA Dependent RNA Polymerase (RdRp)Article Published on 2021-12-082022-10-30 Journal: Molecules [Category] COVID-19, SARS, [키워드] Antiviral applied binding affinities candidate competing Compound computational approach COVID-19 demonstrated Dependent develop DNA DNA polymerase Drug discovery examined FDA-approved drug HIV inhibit inhibitory MD simulation mechanism molecular docking natural product polyphenol Potential RdRP Remdesivir RNA RNA polymerase SARS-CoV SARS-CoV-2 SARS-CoV-2 RNA transcriptase treatments for COVID-19 Virtual screening [DOI] 10.3390/molecules26247438 PMC 바로가기 [Article Type] Article