Bacterial surface lipoproteins mediate epithelial microinvasion by Streptococcus pneumoniae세균 표면 리포단백질은 폐렴구균에 의한 상피 미세 침투를 매개한다.Article Published on 2024-05-072024-08-24 Journal: Infection and Immunity [Category] 폐렴구균 감염증, [키워드] bacterial lipoproteins epithelium host-microbe interactions microinvasion. Streptococcus pneumoniae
Measuring Niche-Associated Metabolic Activity in Planktonic and Biofilm Bacteriaarticle Published on 2023-12-312024-09-01 Journal: Methods in molecular biology (Clifton, N.J.) [Category] 폐렴구균 감염증, [키워드] biofilm carbon source colonization epithelium fermentation metabolism mucosa nasopharynx respiratory tract Sepsis Streptococcus pneumoniae virulence [Article Type] article
Type 2 inflammation reduces SARS-CoV-2 replication in the airway epithelium in allergic asthma through functional alteration of ciliated epithelial cellsArticle Published on 2023-03-292024-09-05 Journal: The Journal of Allergy and Clinical Immunology [Category] update2024, [키워드] airway epithelial cells Asthma children COVID-19 epithelium IL-13 SARS-CoV-2 [DOI] 10.1016/j.jaci.2023.03.021 PMC 바로가기 [Article Type] Article
Exploration of the ocular surface infection by SARS-CoV-2 and implications for corneal donation: An ex vivo studySARS-COV-2에 의한 안구 표면 감염의 탐색 및 각막 기증에 대한 시사점 : 생체 외 연구Research Article Published on 2022-03-012022-09-10 Journal: PLoS Medicine [Category] MERS, SARS, 치료기술, [키워드] 24 hour 24 hours ACE-2 acute respiratory syndrome aims Algorithms angiotensin angiotensin-converting enzyme 2 bulbar conjunctiva cathepsin cellular entry Conjunctiva Contamination contraindication contraindications Cornea Corneal Corneal Graft Corneal transplantation coronavirus coronavirus 2 coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 crisis Ct value Culture culture media decrease diagnosed diagnosed with COVID-19 Donor Donor selection donors enzyme epithelial cell epithelial cells epithelium evaluate Evidence Ex vivo exacerbated exploration expresse expressed expression expression of SARS-CoV-2 expression pattern female genetic material healthy donor immunohistochemistry implication IMPROVE increase in Infection infectious dose inoculation involved lack limbus limited sample size mandatory media median moderate molecular mRNA level nasopharyngeal Nasopharyngeal swab nasopharyngeal swabs Not contaminated Ocular surface organ organ culture pandemic pathway Patient PCR positive Positive test Postinfection promote promoted Protein qPCR quantified quantitative PCR question RdRP receptor Replication researcher respiratory respond retrieved reverse transcription Reverse transcription quantitative PCR risk risk of transmission RNA RNA copy RNA sequence RT-qPCR SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 receptor SARS-CoV-2 receptors SARS-CoV-2 RNA SARS-CoV-2 viral SARS-CoV-2 virus Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 significant increase Support TCID tested tissue tissue culture tissues TMPRSS2 Transmission utility Viral Viral detection Viral RNA was tested were infected worsened [DOI] 10.1371/journal.pmed.1003922 PMC 바로가기 [Article Type] Research Article
The Human Nose Organoid Respiratory Virus Model: an Ex Vivo Human Challenge Model To Study Respiratory Syncytial Virus (RSV) and Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Pathogenesis and Evaluate TherapeuticsResearch Article Published on 2022-02-152022-10-29 Journal: mBio [Category] COVID-19, MERS, [키워드] acute respiratory syndrome Administered Air-liquid interface air-liquid interface (ALI) culture airway Airway organoids airways ALI cultures basolateral compartment challenge cilia ciliary ciliated cell circulation coronavirus coronavirus 2 Culture Cytokines Efficacy epithelium evaluated event Ex vivo feasibility generate Human human airway human respiratory virus human respiratory viruses immunoprophylaxis immunotherapies Infants Infection innate immune responses invasive lung organoid Model Mucus noninvasive technique nose nose organoids occurred Organoid organoids palivizumab Pathogenesis Patient predict Prevent prevented respiratory respiratory syncytial virus respiratory syncytial virus (RSV) respiratory viral respiratory virus RSV SARS-CoV-2 SARS-COV-2 infection severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Therapeutics unmet need viral infection viral shedding [DOI] 10.1128/mbio.03511-21 PMC 바로가기 [Article Type] Research Article
The ACE2 Receptor for Coronavirus Entry Is Localized at Apical Cell—Cell Junctions of Epithelial CellsArticle Published on 2022-02-112022-10-31 Journal: Cells [Category] Coronavirus, [키워드] ACE2 ACE2 mRNA ACE2 protein ADAM17 Apical apical surface association Bacterial Caco-2 CD9 Cell—Cell junctions co-immunoprecipitation coronavirus cultured cell endocytic epithelial epithelial cell epithelial cells epithelium experiments expressed expression identify immunofluorescence microscopy intestinal junction kidney Localized mammary observation promote Protein protein complex Proteins receptor ACE2 target tight junction tissue tissues toxins transmembrane transmembrane protein upregulated virus [DOI] 10.3390/cells11040627 PMC 바로가기 [Article Type] Article
Vectorial Release of Human RNA Viruses from Epithelial CellsReview Published on 2022-01-252023-08-29 Journal: Viruses [Category] E형 간염, [키워드] epithelium human RNA virus polarization release or egress [DOI] 10.3390/v14020231 PMC 바로가기 [Article Type] Review
Deciphering Respiratory-Virus-Associated Interferon Signaling in COPD Airway EpitheliumReview Published on 2022-01-132022-10-30 Journal: Medicina [Category] COVID-19, [키워드] accelerated activate Activation adaptive affecting airway epithelial cell airway epithelial cells airway epithelium Antiviral antiviral response antiviral therapy cascade characterized contribute COPD death Defense Deterioration disease severity effective Epidemic epithelium exhibit facilitate healthy Hospitalization Host IFN IFN signaling Immune homeostasis Immunity impairment increased risk Inflammatory mediators Inhaled Interaction interferon signaling Lower respiratory tract lung lung disorder Lung function mechanism mechanisms Mortality pathogen Patient patients pulmonary inflammation reduce Research respiratory respiratory virus response resulting secondary bacterial infection secreting severe disease Signaling subsequent susceptibility susceptible the interferon therapeutic intervention understanding viral infection virus virus clearance [DOI] 10.3390/medicina58010121 PMC 바로가기 [Article Type] Review
Imiquimod Boosts Interferon Response, and Decreases ACE2 and Pro-Inflammatory Response of Human Bronchial Epithelium in AsthmaImiquimod는 인터페론 반응을 높이고 ACE2 및 천식에서 인간 기관지 상피의 염증 반응을 감소시킵니다Immunology Published on 2021-12-072022-09-11 Journal: Frontiers in Immunology [Category] SARS, 치료법, [키워드] ACE2 ACE2 expression analyses Analysis analyzed Anti-inflammatory Anti-viral anti-viral cytokine anti-viral cytokines anti-viral drug Anti-viral treatment Asthma asthmatic Asthmatic patients baseline bronchial bronchial epithelial cell COVID-19 COVID-19 infection cytokine cytokine signaling Cytokines decrease donors drug dsRNA dual Effect ELISA epithelial epithelial cells epithelium Ex vivo examined exhibit expression feasibility HBEC HBECs healthy highlight Human IFN signaling IFN-β IL-1β IL-33 IL-6 IL-8 imiquimod IMPROVE infections involved mechanism mRNA multiplex objective pathway poly(I:C poly(I:C) pro-inflammatory pro-inflammatory cytokine pro-inflammatory cytokines producing protective effect proteomic qPCR Receptor binding reduced reducing resistance mechanisms response Result RT-qPCR SARS – CoV – 2 SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 receptor Severe COVID-19 Infection SP1 spike-protein stimulated subjects suppression target TLR7 TLR7 agonist Tolerance treat Treatment Trigger triggers Viral viral infection viral infections Viral resistance western blot [DOI] 10.3389/fimmu.2021.743890 PMC 바로가기 [Article Type] Immunology
Effect of Angiotensin-Converting-Enzyme Inhibitor and Angiotensin II Receptor Antagonist Treatment on ACE2 Expression and SARS-CoV-2 Replication in Primary Airway Epithelial CellsPharmacology Published on 2021-11-192022-10-31 Journal: Frontiers in Pharmacology [Category] COVID-19, [키워드] ACE2 ACE2 expression ACEi ACEIs AEC Air-liquid interface airway airway epithelial cell airway epithelium Angiotensin II angiotensin II (A II) receptor antagonists angiotensin-converting eitzyme inhibitors angiotensin-converting enzyme 2 antagonist antagonists ARB binding Biosafety Level 3 BSL-3 Captopril cardiovascular system Cell cells change changed children Clinical data convert Culture culture media cultures disease Effect epithelium Ex vivo expression generate IFN IFNB1 IFNL2 IL-1B IL-6 Infection infection with SARS-CoV-2 inhibitors interferon kidney losartan medication modify modulate MOI multiplicity of infection not significantly different poor prognosis Proinflammatory cytokine protective effect Protein qPCR quantitative PCR receptor reduce Replication responses risk risk of COVID-19 RNA SARS-CoV-2 SARS-CoV-2 replication SARS—CoV—2 significantly the SARS-CoV-2 treated Treatment type I USA-WA1/2020 viral spike protein were assessed were infected with COVID-19 [DOI] 10.3389/fphar.2021.765951 PMC 바로가기 [Article Type] Pharmacology