Metagenomic analysis reveals differences in the co-occurrence and abundance of viral species in SARS-CoV-2 patients with different severity of diseaseArticle Published on 2022-10-192022-11-15 Journal: BMC Infectious Diseases [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] age Analysis cause characterized clinical manifestation co-occurrence condition COVID-19 COVID-19 patient Deceased disease disease severity DNA virus eukaryotic fraction Hospitalized hospitalized patient Human Identified individuals Measle metagenomic sequencing outcome outcomes Patient presence of comorbidities Prevalence prevalent prevalent virus Prognosis respiratory respiratory virus respiratory viruses Result reveal rotavirus Sample SARS-CoV-2 SARS-CoV-2 infected patient SARS-COV-2 infection SARS-CoV-2 patient Severity of disease significantly more torque Virological virus viruses [DOI] 10.1186/s12879-022-07783-8 PMC 바로가기
Calpains in cyanobacteria and the origin of calpains Research Published on 2022-08-162022-10-05 Journal: Scientific Reports [Category] 유전자 메커니즘, [키워드] Anabaena analyses Analysis archaeal Bacteria Bacterial calpain cellular processes cleavage Clusters cysteine protease defined domain Donor enzyme eukaryote eukaryotes eukaryotic explain Human in silico independent information Interaction interaction partner investigated involved mammals Modification Phylogenetic analysis predicted Protein responsible retained selected substrates variety [DOI] 10.1038/s41598-022-18228-2 [Article Type] Research
Computational Analysis of Short Linear Motifs in the Spike Protein of SARS-CoV-2 Variants Provides Possible Clues into the Immune Hijack and Evasion Mechanisms of Omicron VariantSARS-CoV-2 변이체의 스파이크 단백질에서 짧은 선형 모티프의 컴퓨터 분석은 Omicron 변이체의 면역 하이잭 및 회피 메커니즘에 대한 가능한 단서를 제공합니다Article Published on 2022-08-082022-09-11 Journal: International Journal of Molecular Sciences [Category] SARS, 변종, 유전자 메커니즘, [키워드] Affect approach binding cellular interaction cellular pathways Coronaviruses COVID-19 eukaryotic facilitate Host host protein host proteins immune functions information interfere intracellular proteins intracellular signaling linear motif motifs omicron potential therapeutic target potential therapeutic targets Protein protein-protein interaction regulate SARS-CoV-2 SARS-CoV-2 variants sequence Sequence analysis significantly SLiMs Spike protein Spike proteins the SARS-CoV-2 the Spike Transmission variant variants viral infection virus [DOI] 10.3390/ijms23158822 PMC 바로가기 [Article Type] Article
Immunological investigation of a multiepitope peptide vaccine candidate based on main proteins of SARS-CoV-2 pathogenSARS-CoV-2 병원체의 주요 단백질에 기반한 다중 에피토프 펩타이드 백신 후보의 면역학적 조사Article Published on 2022-06-092022-09-11 Journal: PLoS ONE [Category] COVID19(2023년), SARS, 변종, 치료법, [키워드] antigenic Cell complex cytotoxic T lymphocyte develop DNA E. coli Epitopes eukaryotic evaluated expression expression vectors granzyme granzyme B groups helper T lymphocyte Heterologous homologous IFN-γ IgG IgG2a IL-15 IL-21 IL-6 immune response immune responses Immunity immunized in silico in vitro in vivo indicated induce linear B linear B lymphocyte lymphocyte mammalian cell membrane protein mice multiepitope peptide vaccine multiepitope vaccine Multiepitope vaccines nucleocapsid Nucleocapsid phosphoprotein pathogen Pathogens pcDNA3.1 Penetrating peptide produced Protein regimen regimens SARS-CoV-2 SARS-COV-2 infection secretion significantly spike glycoprotein stimulated subcloned T lymphocyte target targets TNF-α Vaccine vaccine candidate variants were used [DOI] 10.1371/journal.pone.0268251 PMC 바로가기 [Article Type] Article
Addition of arginine hydrochloride and proline to the culture medium enhances recombinant protein expression in Brevibacillus choshinensis: The case of RBD of SARS-CoV-2 spike protein and its antibodyarginine hydrochloride와 proline을 배지에 첨가하면 Brevibacillus choshinensis에서 재조합 단백질 발현이 향상됩니다: SARS-CoV-2 스파이크 단백질의 RBD 및 그 항체의 경우Article Published on 2022-06-012022-09-11 Journal: Protein expression and purification [Category] COVID19(2023년), MERS, SARS, 변종, 진단, [키워드] 96-well plates addition Analysis anti-SARS-CoV-2 antibody antibody arginine arginine hydrochloride B.1.1.529 Bacterial bacterial cell bacterial cells bacterial strains Brevibacillus Cell condition contribute CoV-2 Culture culture medium demonstrated ENhance Escherichia coli eukaryotic eukaryotic cell exhibited expressed expression Fab functional functional expression gram-positive bacterium High-throughput expression and purification indicated medium Omicron variant physicochemical produced producing proline Protein protein expression Proteins purification RBD RBD of SARS-CoV-2 RBD. Receptor binding domain recombinant proteins reported SARS-CoV-2 spike protein secrete Spike protein strain the SARS-CoV-2 wild-type [DOI] 10.1016/j.pep.2022.106075 PMC 바로가기 [Article Type] Article
Expression, purification and characterization of SARS-CoV-2 spike RBD in ExpiCHO cellsExpiCHO 세포에서 SARS-CoV-2 스파이크 RBD의 발현, 정제 및 특성화Article Published on 2022-06-012022-09-11 Journal: Protein expression and purification [Category] COVID19(2023년), MERS, SARS, 변종, 진단, [키워드] antibody Antibody binding assay Antigen binding binding domain Blotting collection time Critical Culture culture medium Diagnosis diagnostic effective element ELISA test eukaryotic evaluate evaluated ExpiCHO cell ExpiCHO cells expressed Final flow cytometer functional good quality identify immune response Infection Interferometry Isolation mass mass spectrometry medium monomeric mutants Protein protein production protocol purification purified RBD Receptor binding domain receptor binding domain (RBD) robust SARS-CoV-2 SARS-CoV-2 spike SARS-CoV-2 Spike RBD SARS-CoV-2 Spike. SDS-PAGE selective Spectrometry spike the RBD transiently transfected Vaccines virus western blotting [DOI] 10.1016/j.pep.2022.106071 PMC 바로가기 [Article Type] Article
Phosphate-regulated expression of the SARS-CoV-2 receptor-binding domain in the diatom Phaeodactylum tricornutum for pandemic diagnostics유행성 진단을 위한 규조류 Phaeodactylum tricornutum에서 SARS-CoV-2 수용체 결합 도메인의 인산염 조절 발현Article Published on 2022-04-292022-09-11 Journal: Scientific Reports [Category] COVID19(2023년), SARS, 변종, 진단, 치료기술, [키워드] alleviate anti-RBD antibiotic Antigen approach Betacoronavirus binding Biomedical research C-terminal can be used caused Cell coronavirus COVID-19 pandemic cross-reactive detect develop diagnostics domain Donor eukaryotic expressed expression expression plasmids finding glycosylated growth media histidine human ACE2 receptor inhibited iron lateral flow mammalian cell overexpression pandemic performed phosphate Plasmid polyclonal antibodies promoter purified RBD Receptor-binding domain retained SARS-CoV-2 SARS-CoV-2 proteins SARS-CoV-2 spike protein sensitivity serum specific IgG antibodies specific IgG antibody Spike protein Synthetic biology TEV protease the RBD the receptor-binding domain the SARS-CoV-2 Therapeutics Treatment type strain Viral antigen wild-type strain yield [DOI] 10.1038/s41598-022-11053-7 PMC 바로가기 [Article Type] Article
Could the COVID-19-Driven Increased Use of Ivermectin Lead to Incidents of Imbalanced Gut Microbiota and Dysbiosis?COVID-19로 인해 증가된 Ivermectin 사용이 장내 미생물 불균형 및 Dysbiosis를 유발할 수 있습니까?Review Published on 2022-04-012022-09-11 Journal: Probiotics and antimicrobial proteins [Category] COVID19(2023년), SARS, 치료제, [키워드] Affect animals COVID-19 diagnosed with COVID-19 drug Dysbiosis eukaryotic gut microbiota hosts humans incident Increased individual Infection Ivermectin nucleus SARS-CoV-2 SARS-COV-2 infection suggestion susceptible Treatment virus [DOI] 10.1007/s12602-022-09925-5 PMC 바로가기 [Article Type] Review
SARS-CoV-2 proteome microarray for COVID-19 patient sera profilingCOVID-19 환자 혈청 프로파일링을 위한 SARS-CoV-2 프로테옴 마이크로어레이Article Published on 2022-02-282022-09-12 Journal: STAR protocols [Category] COVID19(2023년), MERS, SARS, 진단, [키워드] acute respiratory syndrome Affinity purification Antibody Response Biotechnology Biotechnology and bioengineering build Cell clinical clinical protocol Complete coronavirus COVID-19 patient E. coli eukaryotic expression facilitate Health Sciences help IgG and IgM Immunity immunology include Microarray Protein Protein Biochemistry Protein expression and purification proteome proteomics protocol SARS-CoV-2 SARS-CoV-2 proteome sera were expressed [DOI] 10.1016/j.xpro.2022.101238 PMC 바로가기 [Article Type] Article
In silico Analysis of SARS-CoV-2 ORF8-Binding Proteins Reveals the Involvement of ORF8 in Acquired-Immune and Innate-Immune SystemsMedicine Published on 2022-02-012022-10-31 Journal: Frontiers in Medicine [Category] COVID-19, [키워드] Analysis Antigen Autoimmunity binding candidate cellular class Cluster Clustering analysis coagulation cascade complement conserved contribute coronavirus Coronavirus infection COVID-19 COVID-19 pathogenesis dataset Degradation described downregulation Endoplasmic reticulum eukaryote eukaryotic genome sequence homology immune evasion in silico analysis Infection involved Involvement KEGG KEGG pathway analysis less leukocyte ORF8 ORF8 accessory gene ORF8 protein pathology performed Phylogenetic phylogenetic profiling physically predicted promote Protein Proteins SARS-CoV SARS-CoV-2 severe symptom Spread Stress suggested System tissue-specific expression variant of SARS-CoV-2 ward was performed wild-type SARS-CoV-2 [DOI] 10.3389/fmed.2022.824622 PMC 바로가기 [Article Type] Medicine