Quality assessment of histopathological stainings on prolonged formalin fixed thrombus tissues retrieved by mechanical thrombectomyNeurology Original Research Published on 2023-12-132024-09-05 Journal: Frontiers in Neurology [Category] update2024, [키워드] formalin Histopathology Ischemic Stroke Mechanical thrombectomy thrombosis [DOI] 10.3389/fneur.2023.1223947 PMC 바로가기 [Article Type] Neurology Original Research
SARS-CoV-2 RNA detection in Formalin-Fixed Paraffin-Embedded (FFPE) tissue by droplet digital PCR (ddPCR)물방울 디지털 PCR(ddPCR)에 의한 포르말린 고정 파라핀 포매(FFPE) 조직에서 SARS-CoV-2 RNA 검출Article Published on 2022-07-012022-09-11 Journal: Clinica chimica acta; international journal of cli [Category] COVID19(2023년), SARS, 치료기술, 치료법, 치료제, [키워드] amplified Atypical Bio-rad cause clinical utility clinically COVID-19 digital PCR droplet droplet digital PCR extraction fetal FFPE formalin formalin-fixed paraffin-embedded highest Infection limit of detection liver liver transplant liver transplant rejection material paraffin Placenta Placental viral infection positive positive sample Pregnancy qualitative test quantified rejection reported Respiratory disease reverse transcription RNA virus RPP30 SARS-CoV-2 SARS-CoV-2 nucleocapsid SARS-CoV-2 RNA detection sequence specimen specimens tested tissue Tissue-based viral detection total RNA transplant rejection Viral detection Viral detection in FFPE. viral infection [DOI] 10.1016/j.cca.2022.05.007 PMC 바로가기 [Article Type] Article
Post-mortem histopathology of pituitary and adrenals of COVID-19 patientsCOVID-19 환자의 뇌하수체 및 부신의 사후 조직 병리학Article Published on 2022-07-012022-09-11 Journal: Legal medicine (Tokyo, Japan) [Category] 치료기술, 치료법, [키워드] addition Adrenal adrenal gland adrenals analyzed antibody Cell changes in cortical Course COVID-19 COVID-19 infection COVID-19 patient Evidence evidence of examined formalin histopathological Histopathology increase in independent of Infection lymphoma Necrosis no evidence of nodule nucleocapsid organ PAS performed Pituitary pituitary gland Pituitary. predominant Shock staining Stress stress response T-cell T-Cell Lymphoma tissue [DOI] 10.1016/j.legalmed.2022.102045 PMC 바로가기 [Article Type] Article
An Artificial Intelligence-guided signature reveals the shared host immune response in MIS-C and Kawasaki disease인공 지능 유도 서명은 MIS-C 및 가와사키병에서 공유된 숙주 면역 반응을 나타냅니다.Article Published on 2022-05-162022-09-12 Journal: Nature Communications [Category] COVID19(2023년), MERS, SARS, 바이오마커, 유전자 메커니즘, 진단, 치료법, [키워드] artificial cardiac function children clinical feature continuum COVID-19 COVID-19 pandemic cytokine Cytokine storm demonstrated diagnostic fixed formalin gene signature Host immune response Inflammatory Kawasaki disease Laboratory laboratory parameter MIS-C MONITOR pandemic parameter pathway pediatric Phenotypes proximal reduced Result reveal RNA sequences SARS-COV-2 infection serum cytokines severity syndrome tissues unique Whole blood [DOI] 10.1038/s41467-022-30357-w PMC 바로가기 [Article Type] Article
Multi-Design Differential Expression Profiling of COVID-19 Lung Autopsy Specimens Reveals Significantly Deregulated Inflammatory Pathways and SFTPC Impaired TranscriptionCOVID-19 폐 부검 표본의 다중 디자인 차등 발현 프로파일링은 상당히 규제 완화된 염증 경로 및 SFTPC 손상된 전사를 나타냅니다Article Published on 2022-03-162022-09-11 Journal: Cells [Category] SARS, 유전자 메커니즘, [키워드] Activation Autopsy carried cathepsin Cathepsin C CD163 CFB Clinical diagnosis complement Complement activation controls COVID-19 COVID-19 patient COVID-19-related death CTSC deaths deregulation develop differential expression effective effective therapy FFPE formalin formalin-fixed paraffin-embedded Gene expression profiling had no high viral load IFIT1 immune signature immunohistochemistry Impaired Inflammation ISG15 lung lung damage Macrophage macrophage polarization mechanism MS4A4A Nasopharyngeal swab OAS1 overexpression paraffin pathway pharmacological strategies PKM positive potential therapeutic targets Prevent probe Profiling Protein protein C pulmonary viral quantified required Respiratory disease RNA RNA-Seq RSAD2 SARS-CoV-2 SARS-COV-2 infection selected Seven SFTPC Siglec-1 SIGLEC1 signaling pathway specimen tissue sample tissue samples transcriptomic Transcriptomic profiling type I interferon Viral load were used [DOI] 10.3390/cells11061011 PMC 바로가기 [Article Type] Article
Subcellular Detection of SARS-CoV-2 RNA in Human Tissue Reveals Distinct Localization in Alveolar Type 2 Pneumocytes and Alveolar MacrophagesResearch Article Published on 2022-02-082022-10-29 Journal: mBio [Category] COVID-19, MERS, [키워드] AGER alveolar Alveolar macrophages amplification AT1 AT2 Autopsy caused Cell Cell culture cell line Cellular imaging coronavirus disease COVID-19 detection diagnostics Drug screening FFPE fixation fluorescent image analysis formalin Human human lung tissue human tissue identify infected patients infected with SARS-CoV-2 Infection Localization lung Lymph node macrophage marker Marco marker gene marker genes novel coronavirus SARS-CoV-2 observé ORF1a paraffin embedding pathology performed Placenta platform pneumocyte probe Region regions RNA RNA FISH SARS-CoV-2 SARS-CoV-2 RNA SFTPC Single Cell specimens subcellular subcellular localization subgenomic mRNAs tissue tissues Type viral life cycle Viral RNA widespread [DOI] 10.1128/mbio.03751-21 PMC 바로가기 [Article Type] Research Article
Variable levels of spike and ORF1ab RNA in post-mortem lung samples of SARS-CoV-2-positive subjects: comparison between ISH and RT-PCRSARS-CoV-2 양성 대상자의 사후 폐 샘플에서 다양한 수준의 스파이크 및 ORF1ab RNA: ISH와 RT-PCR 간의 비교Original Article Published on 2022-02-012022-09-10 Journal: Virchows Archiv [Category] COVID19(2023년), MERS, SARS, 치료기술, 치료법, 치료제, [키워드] amplification amplification cycle amplification cycles analyzed cellular Chain Reaction correlation Deceased demonstrated detect E gene electron microscopy envelope FFPE formalin formalin-fixed paraffin-embedded Frame gene amplification IHC immunohistochemistry In situ hybridization In situ hybridization (ISH) Microscopy morphology N gene nucleocapsid Open reading frame Open reading frame (ORF1ab) optimization ORF1 ORF1ab ORF1ab gene paraffin polymerase chain polymerase chain reaction positive Post-mortem post-mortem lung Post-mortem lung samples reverse transcription reverse transcription polymerase chain reaction Reverse transcription polymerase chain reaction (RT-PCR) RNA RT-PCR S gene SARS-CoV-2 sensitivity sequence Spike(S) staining subjects supplementary material the SARS-CoV-2 tissue variable Viral Viral RNA virus virus detection [DOI] 10.1007/s00428-021-03262-8 PMC 바로가기 [Article Type] Original Article
Pro-inflammatory microenvironment and systemic accumulation of CXCR3+ cell exacerbate lung pathology of old rhesus macaques infected with SARS-CoV-2CXCR3+ 세포의 염증 유발 미세 환경 및 전신 축적은 SARS-CoV-2에 감염된 늙은 붉은털 원숭이의 폐 병리를 악화시킵니다.Article Published on 2021-09-012022-09-10 Journal: Signal Transduction and Targeted Therapy [Category] Fulltext, SARS, 유전자 메커니즘, 진단, [키워드] Activation acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Analysis angiotensin Angiotensin-converting enzyme angiotensin-converting enzyme 2 animal model Apoptosis autophagy both lungs CD8+ cell CD8+ cells Cell cells changes in Chemokine receptor coronavirus coronavirus disease Coronavirus disease 2019 correlated COVID-19 CXCR3 demonstrated elevated exacerbate feature FFPE formalin formalin-fixed paraffin-embedded IFN-α IL-6 immunopathological immunopathology infected with SARS-CoV-2 Infection Infectious diseases interferon interleukin interleukin 6 lung Lung pathology lung tissue Lungs microenvironment motif multiplex immunofluorescence NF-κB nuclear paraffin Peripheral blood pro-inflammatory protein expression Quantitative analysis respiratory rhesus macaque rhesus macaques SARS-CoV-2 SARS-COV-2 infection severe acute respiratory syndrome Coronavirus severe COVID-19 significantly staining T cell T-box tissue Treatment understanding [DOI] 10.1038/s41392-021-00734-w PMC 바로가기 [Article Type] Article
A human cell-based SARS-CoV-2 vaccine elicits potent neutralizing antibody responses and protects mice from SARS-CoV-2 challenge인간 세포 기반 SARS-CoV-2 백신은 강력한 중화 항체 반응을 유도하고 SARS-CoV-2 공격으로부터 마우스를 보호합니다Research Article Published on 2021-08-122022-09-10 Journal: Emerging Microbes & Infections [Category] SARS, 변종, 진단, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 administration ameliorated Antibody titer approach caused Cell cell-based vaccines cellular convalescent serum coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 demonstrated develop effective Efficacy elicit exposure to external gene external genes formalin generate greater hACE2 human cell immunization in viral inactivated Inactivated vaccine induce K562-S Log reduction lung Lung pathology membrane MERS-CoV mice mouse model NAb neutralizing antibody Neutralizing antibody response Neutralizing antibody titer non-human primate non-human primate model non-human primates pandemic platform promise PROTECT protection Protective Protein regimen respiratory response S-protein Safe SARS-CoV SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 mutants SARS-CoV-2 vaccine severe acute respiratory syndrome Coronavirus spike Support turn vaccination Vaccine vaccine candidate vectors viability Viral X-ray [DOI] 10.1080/22221751.2021.1957400 PMC 바로가기 [Article Type] Research Article
Multisystemic Cellular Tropism of SARS-CoV-2 in Autopsies of COVID-19 PatientsCOVID-19 환자의 부검에서 SARS-CoV-2의 다중 전신 세포 친화성Article Published on 2021-07-272022-09-11 Journal: Cells [Category] SARS, 치료기술, [키워드] ACE2 adrenal gland alteration analyzed assigned Autopsies Autopsy bowel Cell Chain Reaction correlation COVID-19 COVID-19 autopsy sample electron microscopy endothelial cells epithelial epithelial cell epithelial cells expressing fatal COVID-19 FFPE formalin formalin-fixed paraffin-embedded formalin-fixed paraffin-embedded (FFPE) tissue heart histology histopathological immunohistochemistry In situ hybridization Infection kidney liver lung lymph nodes major organs Microscopy organ systems organs oropharynx paraffin polymerase chain polymerase chain reaction Post-mortem Prostate Replication respiratory respiratory tract RNA salivary glands SARS-CoV-2 SARS-CoV-2 detection SARS-COV-2 infection SARS-CoV-2 RNA shown Skeletal muscle skin the SARS-CoV-2 Thyroid TMPRSS2 tonsils Trachea transmission electron microscopy tropism tropism of SARS-CoV-2 Viral Viral detection Viral protein Viral RNA virus particle virus particles [DOI] 10.3390/cells10081900 PMC 바로가기 [Article Type] Article