Mulberry Component Kuwanon C Exerts Potent Therapeutic Efficacy In Vitro against COVID-19 by Blocking the SARS-CoV-2 Spike S1 RBD:ACE2 Receptor InteractionArticle Published on 2022-10-192022-11-15 Journal: International Journal of Molecular Sciences [Category] COVID19(2023년), SARS, 신약개발, 치료제, [키워드] ACE2 ACE2 receptor acute respiratory syndrome addition anticipated antiviral efficacy blocker blocking cell entry Clinical use Combination component coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 demonstrated develop disrupting docking drugs effective Effectiveness Efficacy effort ELISA ENhance Future HEK293T cells in silico Infection inhibit inhibitor inhibitors Interaction isolate kuwanon C lack mechanism of action Morus Alba Morus alba L. potent Prevent Pseudotyped virus receptor Replication RNA SARS-CoV-2 SARS-CoV-2 spike several limitation severe acute respiratory syndrome coronavirus 2 significantly spike Spike protein spike S1 RBD supported target tested the SARS-CoV-2 Therapies Vero cells [DOI] 10.3390/ijms232012516 PMC 바로가기
Using the Sleeping Beauty (SB) Transposon to Generate Stable Cells Producing Enveloped Virus-Like Particles (eVLPs) Pseudotyped with SARS-CoV-2 Proteins for VaccinationArticle Published on 2022-10-012023-07-10 Journal: Current protocols [Category] COVID19(2023년), [키워드] enveloped VLPs (eVLPs) HEK293T cells Sleeping Beauty transposon stable mammalian cell line Virus-like particles (VLPs) your favorite gene (YFG). [DOI] 10.1002/cpz1.575 PMC 바로가기
Human surfactant protein D facilitates SARS-CoV-2 pseudotype binding and entry in DC-SIGN expressing cells, and downregulates spike protein induced inflammation인간 계면활성제 단백질 D는 DC-SIGN 발현 세포에서 SARS-CoV-2 유사형 결합 및 진입을 촉진하고 스파이크 단백질 유도 염증을 하향 조절합니다Article Published on 2022-07-282022-09-11 Journal: Frontiers in Immunology [Category] COVID19(2023년), SARS, 유전자 메커니즘, 치료기술, [키워드] ACE2 Analysis Anti-viral binding carbohydrate cause Cell cell interaction cell line cell surface receptor cells chemokine chemokines collectins controls COVID-19 cytokine cytokine response Cytokines DC-SIGN dendritic cell domain downregulate downregulated eight epithelial expressing facilitate fragment of HEK 293T HEK293T cell HEK293T cells help Human IFN-α IL- 6 IL-1β IL-8 immune function immune response immunomodulatory effect increase independent independent of infection assay infection assays Inflammation inhibit SARS-CoV-2 innate immune innate immune system Interaction intercellular involved Macrophage macrophages MHC class MHC class II modulating mRNA expression mRNA level mRNA levels N-terminal naïve T cell naïve T cells NF-κB pathogen Pathogens pro-inflammatory cytokine pro-inflammatory cytokines Protein pseudotype RANTES receptors Replication rfhSP-D. RNA virus RT-qPCR SARS- CoV-2 SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 replication SARS-CoV-2 spike protein shown SIGN spike Spike protein surface receptor T cells the Spike THP-1 TNF-α treated Treatment Vero Cell Vero cells viral entry viruses [DOI] 10.3389/fimmu.2022.960733 PMC 바로가기 [Article Type] Article
Photodynamic disinfection of SARS-CoV-2 clinical samples using a methylene blue formulationArticle Published on 2022-06-012022-10-04 Journal: Photochemical & photobiological sciences : Official journal of the European [Category] COVID19(2023년), SARS, 신약개발, [키워드] amplification amplitude antimicrobial Antimicrobial photodynamic disinfection approved clinical sample Clinical use collected Concentration condition coronavirus disease COVID-19 COVID-19 patients cytotoxicity early stage exhibiting form global effort HEK293T cells infect Infection lentiviral luciferase reporter gene medical need Medicines Methylene Methylene blue nasal pandemic PDI photodisinfection photodynamic inactivation prevented pseudotyped Pseudotyped lentiviral vector reduce SARS-CoV-2 severity the disease the S protein Transmission Treatment urgency Viral load [DOI] 10.1007/s43630-022-00202-6 PMC 바로가기 [Article Type] Article
Reduced DMPC and PMPC in lung surfactant promote SARS-CoV-2 infection in obesity폐 계면활성제의 감소된 DMPC 및 PMPC는 비만에서 SARS-CoV-2 감염을 촉진합니다Article Published on 2022-06-012022-09-11 Journal: Metabolism: clinical and experimental [Category] COVID19(2023년), SARS, 변종, [키워드] ACE2 ACE2 expression ACE2 receptor Analysis BALF bind Bronchoalveolar lavage fluid cell-cell fusion assay characterized cholesterol COVID-19 COVID-19 disease COVID-19 pneumonia D614G mutant SARS-CoV-2 Defense DMPC Effect effector cells elevated expressing fat Feeding HEK293T HEK293T cell HEK293T cells High fat diet Hospitalization increased risk Increasing Infection infections inhibited inhibitory effect Inhibitory effects Lipid lipid composition lipid metabolism lipids lung lung surfactant lung tissue Lungs mechanism metabolism mice myristic acid obese obesity outcomes overexpression Phosphatidylcholine Pneumocytes produced promote Protein reduced respiratory virus reversed risk factor SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 pseudovirus infection SARS-CoV-2 viral loads severe COVID-19 disease Severe COVID-19 pneumonia Spike protein surfactant the Spike Triglyceride triglycerides trimyristin Type II pneumocyte type II pneumocytes Vero-E6 cell Vero-E6 cells was performed were used wild type [DOI] 10.1016/j.metabol.2022.155181 PMC 바로가기 [Article Type] Article
Neutralization or enhancement of SARS-CoV-2 infection by a monoclonal antibody targeting a specific epitope in the spike receptor-binding domain스파이크 수용체 결합 도메인의 특정 에피토프를 표적으로 하는 단일클론항체에 의한 SARS-CoV-2 감염의 중화 또는 증강Article Published on 2022-04-012022-09-11 Journal: Antiviral Research [Category] COVID19(2023년), MERS, SARS, 변종, 진단, [키워드] abrogated ACE2 acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Alanine Alpha angiotensin Angiotensin-converting enzyme angiotensin-converting enzyme 2 antibody Antibody-dependent enhancement binding binding epitope caused cellular contrary coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 cross-reactivity Delta demonstrated dose-dependent manner ELISA epitope Epitopes Epsilon exhibited exhibiting experiment Gamma HEK293T cells Infection lentivirus MERS-CoV monoclonal antibodies monoclonal antibody mouse monoclonal antibody NAbs Neutralizing activity Neutralizing antibodies neutralizing antibody Prophylactic Protein pseudotyped RBD receptor Receptor binding domain Receptor-binding domain reduction Region reported residue residues respiratory S protein S proteins SARS-CoV SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 spike SARS-CoV-2 variant SARS-CoV-2 variants severe acute respiratory syndrome Coronavirus spike Spike receptor binding domain Spike receptor binding domain. spike receptor-binding domain the RBD the receptor-binding domain the S protein Therapeutic treatment variant variants of SARS-CoV-2 wild-type [DOI] 10.1016/j.antiviral.2022.105290 PMC 바로가기 [Article Type] Article
Enoxaparin augments alpha-1-antitrypsin inhibition of TMPRSS2, a promising drug combination against COVID-19Enoxaparin은 COVID-19에 대한 유망한 약물 조합인 TMPRSS2의 알파-1-항트립신 억제를 증가시킵니다.Article Published on 2022-03-252022-09-11 Journal: Scientific Reports [Category] SARS, 변종, 진단, 치료법, [키워드] affected alpha-1-antitrypsin anti-inflammatory activity antitrypsin Cell cells cleave complex consequence Coronavirus infection COVID-19 crystal structure drug combination ENhance enoxaparin epithelial cell epithelial cells hAEC HCoV HCoV-229E HEK293T cell HEK293T cells heparin human coronavirus human coronavirus 229E immunostaining Infection Inflammatory less molecular nafamostat Neutralizing nucleocapsid protein overexpressing Plaque assay primary human airway Primary human airway epithelial cells quantified reactive required SARS-CoV-2 SARS-CoV-2 mutations Serine serine protease severe COVID-19 Spike protein the cell the spike protein therapy TMPRSS2 TMPRSS2 inhibitor Treatment Viral viral entry virus was determined [DOI] 10.1038/s41598-022-09133-9 PMC 바로가기 [Article Type] Article
A cell-based system combined with flow cytometry to evaluate antibody responses against SARS-CoV-2 transmembrane proteins in patients with COVID-19코로나19 환자의 SARS-CoV-2 막관통 단백질에 대한 항체 반응을 평가하기 위해 유세포 분석과 결합된 세포 기반 시스템Article Published on 2022-02-222022-09-11 Journal: STAR protocols [Category] COVID19(2023년), SARS, 변종, [키워드] antibody Antibody Response Antibody responses approach Biology Cell Biology Cell-based Assays Complete Effectiveness evaluate Flow flow cytometry Flow Cytometry/Mass Cytometry glycosylation Health Sciences HEK293T cell HEK293T cells host cells insertion Laboratory membrane membrane protein Microbiology Molecular biology N-glycosylation patient sera patients with COVID-19 powerful tool protocol SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2 variants transmembrane protein Vaccine viral infection [DOI] 10.1016/j.xpro.2022.101229 PMC 바로가기 [Article Type] Article
The interactions of ZDHHC5/GOLGA7 with SARS-CoV-2 spike (S) protein and their effects on S protein’s subcellular localization, palmitoylation and pseudovirus entrySARS-COV-2 스파이크 (S)와 ZDHHC5/GOLGA7의 상호 작용 및 S 단백질의 세포 내 위치, 팔미 토일 화 및 슈도 바이러스 진입에 미치는 영향Research Published on 2021-12-272022-09-10 Journal: Virology Journal [Category] SARS, 치료기술, [키워드] A549 acute respiratory syndrome acute respiratory syndrome coronavirus Affect affected Affinity purification analyzed Antiviral APT2 assays Biotin Cas9 Cell cell line cell lines cell types cells co-immunoprecipitation co-IP Colocalization complex coronavirus CRISPR CRISPR-Cas9 cysteine cysteine residue determine domain Effect Efficiency endodomain enzymatic activity enzyme fluorescence microscopy GOLGA7 had no HEK293T HEK293T cell HEK293T cells Hela HeLa cells host protein host proteins independent independent of interact Interaction knowledge mass spectrometry no significant effect palmitoylation Protein provide pseudovirus entry reason Renilla Renilla luciferase respiratory Result S protein S proteins SARS-CoV-2 SARS-CoV-2 pseudovirus SARS-CoV-2 S protein SARS-CoV-2 spike severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 significant effect significantly Spike protein subcellular localization the cell the S protein virus entry virus infection Virus-host interaction ZDHHC5 ZDHHC5/GOLGA7 [DOI] 10.1186/s12985-021-01722-w PMC 바로가기 [Article Type] Research
Compound screen identifies the small molecule Q34 as an inhibitor of SARS-CoV-2 infectionArticle Published on 2021-12-242022-10-30 Journal: iScience [Category] COVID-19, [키워드] Abstract approved astrocytes calu-3 cells Cell Biology cell entry cellular entry Chemistry Compound COVID-19 COVID-19 outbreak entry of SARS-CoV-2 global public health HEK293T cells human cell human cells human lung identify Infection inhibit inhibitor inhibitors of SARS-CoV-2 Neuron non-toxic Pandemics Prevent pseudotyped pseudotyped SARS-CoV-2 Q34 related virus safety profile SARS-CoV-2 SARS-COV-2 infection screened small molecule Toxicity treated uninfected cell Virology [DOI] 10.1016/j.isci.2021.103684 PMC 바로가기 [Article Type] Article