Antigenic cartography of SARS-CoV-2 reveals that Omicron BA.1 and BA.2 are antigenically distinctArticle Published on 2022-09-232022-11-15 Journal: Science Immunology [Category] SARS, 변종, 진단, [키워드] Affect antigenic Antisera Beta booster Calu-3 Calu-3 cell cell line Characteristics Cluster Clustering Delta Efficacy Gamma Genetic human airway Map Neutralization assay not affect omicron outliers Primary infection replacing reveal SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2 variants sequence spike mutations Spread Topology Vaccine vaccine-induced antibody response Vaccines variant viruses [DOI] 10.1126/sciimmunol.abq4450 PMC 바로가기
Recapitulating infection, thermal sensitivity and antiviral treatment of seasonal coronaviruses in human airway organoids인간 기도 오르가노이드에서 계절성 코로나바이러스의 감염, 열 민감도 및 항바이러스 치료 요약Article Published on 2022-07-012022-09-11 Journal: EBioMedicine [Category] 치료제, [키워드] 229E Airway organoids Analysis antiviral activity antiviral therapy Antiviral treatment approved conditions Coronavirus infection Coronavirus infections COVID COVID19 development experimental model facilitate favor funding help host response Human human airway Infection infections Infectious virus inhibited interactions Kinetics medication medications Mild molnupiravir Netherland NL63 OC43 occur organization Organoid Pathogenesis phenotype populations Remdesivir replicate Replication Research research and development resulting RNA sequencing robust seasonal seasonal coronavirus seasonal coronaviruses severe complication severe complications Society Support supported temperature tested therapeutic thermal Thermal sensitivity Transcriptome transcriptomic Transmissibility upper respiratory tract viral replication virus replication Virus-host interaction Virus-host interactions was performed was tested [DOI] 10.1016/j.ebiom.2022.104132 PMC 바로가기 [Article Type] Article
Sequence-Specific Features of Short Double-Strand, Blunt-End RNAs Have RIG-I- and Type 1 Interferon-Dependent or -Independent Anti-Viral Effects짧은 이중 가닥, 평활 말단 RNA의 서열 특이적 특징은 RIG-I 및 유형 1 인터페론 의존성 또는 독립적 항바이러스 효과를 갖는다Article Published on 2022-06-282022-09-11 Journal: Viruses [Category] COVID19(2023년), SARS, 유전자 메커니즘, 치료법, 치료제, [키워드] adaptors Adenovirus Anti-viral anti-viral effect Antiviral Antiviral agents Antiviral effect antiviral effects assays Cell cell cycle cell cycle genes cell line cells cellular complementary cytoplasmic DNA DNA virus downregulated dsRNA dsRNAs Effect elicited fibroblast Gene Expression H3N2 human airway IFN IFN-alpha IFN-I IFNAR2 independent independent of indicated Infection Influenza influenza A influenza A virus Influenza virus interferon knock-out library MDA5 mechanism mimicking molecular patterns MYD88 organism Organisms pathogen pathogen-associated molecular patterns Pathogens Randomized receptor required retinoic acid retinoic acid-inducible gene I RIG-I RNA RNA therapy RNA virus RNAs SARS-CoV-2 sequence short double-strand blunt-end RNA. transcriptomics transfection Type ZNFX1 [DOI] 10.3390/v14071407 PMC 바로가기 [Article Type] Article
Acriflavine, a clinically approved drug, inhibits SARS-CoV-2 and other betacoronaviruses임상적으로 승인된 약물인 Acriflavine은 SARS-CoV-2 및 기타 베타 코로나바이러스를 억제합니다.Article Published on 2022-05-192022-09-11 Journal: Cell chemical biology [Category] COVID19(2023년), SARS, 신약개발, 치료제, [키워드] ACF acriflavine approved Betacoronavirus betacoronaviruses binding block catalytic caused cellular clinical trial clinical trials clinically Concentration coronavirus COVID-19 COVID-19 pandemic drug Drug repurposing effective effort epithelia Ex vivo human airway human airway epithelia identify in some in vivo inhibit inhibitor limit mice Mortality NMR Outbreaks pandemic Papain-like protease PL(pro) protease Protease inhibitor Research SARS-CoV-2 severe disease structural biology tested Vaccines viral replication [DOI] 10.1016/j.chembiol.2021.11.006 PMC 바로가기 [Article Type] Article
Delta spike P681R mutation enhances SARS-CoV-2 fitness over Alpha variant델타 스파이크 P681R 돌연변이는 알파 변종보다 SARS-CoV-2 적합성을 향상시킵니다.Article Published on 2022-05-172022-09-11 Journal: Cell Reports [Category] COVID19(2023년), SARS, 변종, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Alpha variant Amino acid cleavage coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 CP: Microbiology Delta delta variant ENhance epithelial cell epithelial cells fitness full-length furin furin cleavage site human airway human airway culture human lung human lung epithelial cells IMPROVE Microbiology Mutation P681 P681H P681R pandemic primary human airway reduce reduced Replication S1 and S2 S1/S2 S1/S2 cleavage SARS-CoV-2 severe acute respiratory syndrome Coronavirus significantly spike mutation spike P681R subunits tissues variant viral entry virus entry wild-type [DOI] 10.1016/j.celrep.2022.110829 PMC 바로가기 [Article Type] Article
SARS-CoV-2 Virion Infectivity and Cytokine Production in Primary Human Airway Epithelial Cells1차 인간 기도 상피 세포에서 SARS-CoV-2 비리온 감염성과 사이토카인 생산Article Published on 2022-05-022022-09-11 Journal: Viruses [Category] COVID19(2023년), SARS, 변종, [키워드] Activation Alpha B.1.1.529 B.1.1.7 B.1.351 B.1.617.2 BavPat1 Behavior Beta Cell cultures cytokine D614G mutation epithelial epithelial cell epithelial cells Evidence Ex vivo hAEC Human human airway human airway epithelial infect Infectivity Innate immunity isolate lack Lineage lineages new SARS-CoV-2 no difference observé Omicron variant Omicron variants organotypic model Physiology primary human airway Primary human airway epithelial cells Production provide replacement replicate respiratory tissue respiratory tissues SARS-CoV-2 SARS-CoV-2 variants variant variants variants of concern virion virus vRNA [DOI] 10.3390/v14050951 PMC 바로가기 [Article Type] Article
SARS-CoV-2 infection of airway cells causes intense viral and cell shedding, two spreading mechanisms affected by IL-13기도 세포의 SARS-CoV-2 감염은 IL-13에 의해 영향을 받는 두 가지 확산 메커니즘인 강력한 바이러스 및 세포 방출을 유발합니다Article Published on 2022-04-192022-09-11 Journal: Proceedings of the National Academy of Sciences of [Category] COVID19(2023년), SARS, 유전자 메커니즘, [키워드] ACE2 ACE2 expression acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 affected airway airways Allergic asthma Analysis angiotensin Angiotensin-converting enzyme angiotensin-converting enzyme 2 Antiviral Asthma asthmatic cause caused Cell Cell culture cell cultures cell tropism cells ciliated cell ciliated cells coronavirus COVID-19 COVID-19 severity down-regulated gene down-regulated genes electron microscopy enzyme epithelial epithelial cells examined expression gene expression analyses Gene Expression Omnibus Genes glycoprotein HAE high risk host–virus interaction human airway human airway epithelial human airway epithelial cells IL-13 IL-13 cytokine immunohistochemistry In situ hybridization infected cells Infection interferon interleukin interleukin-13 Intracellular involved keratan sulfate Lung disease mechanism Messenger RNA Microscopy MUC5AC mucin Mucus number of infected primary human airway reduced removal Replication RNA RNA-sequencing SARS-CoV-2 SARS-COV-2 infection severe acute respiratory syndrome Coronavirus shedding shielding significantly Spread sulfate susceptibility Transmission Transport TRAP treated up-regulated viral egress viral entry Viral load Viral reservoir viral spread viral titers virions viruses were infected [DOI] 10.1073/pnas.2119680119 PMC 바로가기 [Article Type] Article
The OM-85 bacterial lysate inhibits SARS-CoV-2 infection of epithelial cells by downregulating SARS-CoV-2 receptor expressionOM-85 박테리아 용해물은 SARS-CoV-2 수용체 발현을 하향 조절하여 상피 세포의 SARS-CoV-2 감염을 억제합니다Article Published on 2022-03-012022-09-11 Journal: The Journal of Allergy and Clinical Immunology [Category] SARS, 비임상, 유전자 메커니즘, [키워드] ACE2 ACE2 protein acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus-2 administration airway airway epithelial cells angiotensin Angiotensin-converting enzyme angiotensin-converting enzyme 2 Bacteria Bacterial bacterial lysate binding bronchial epithelial cells caused Cell cell entry cell interactions cell lines coronavirus disease Coronavirus disease 2019 Coronavirus-2 COVID-19 downregulated downregulating downregulation Effect enzyme epithelial epithelial cell epithelial cell line epithelial cells expression HEK293T cell host cells human airway human epithelial cell human epithelial cells immunomodulating in vitro infect Infection inhibit inhibited Interaction intestinal kidney lentiviral particles lentivirus lung mRNA Multiple OM-85 organ organs Prevent primary bronchial epithelial cells primary human bronchial epithelial cells protease PROTECT Protein Protein binding protein expression pseudotyped lentiviral particles reduce respiratory infections S protein S1 protein safety profile SARS-CoV-2 SARS-CoV-2 cell infection SARS-COV-2 infection SARS-CoV-2 receptor SARS-CoV-2 S SARS-CoV-2 S protein SARS-CoV-2 S1 protein Serine serine 2 Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus severity significantly TMPRSS2 Transcription transmembrane transmembrane protease serine 2 treated Treatment were measured [DOI] 10.1016/j.jaci.2021.11.019 PMC 바로가기 [Article Type] Article
The Human Nose Organoid Respiratory Virus Model: an Ex Vivo Human Challenge Model To Study Respiratory Syncytial Virus (RSV) and Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Pathogenesis and Evaluate TherapeuticsResearch Article Published on 2022-02-152022-10-29 Journal: mBio [Category] COVID-19, MERS, [키워드] acute respiratory syndrome Administered Air-liquid interface air-liquid interface (ALI) culture airway Airway organoids airways ALI cultures basolateral compartment challenge cilia ciliary ciliated cell circulation coronavirus coronavirus 2 Culture Cytokines Efficacy epithelium evaluated event Ex vivo feasibility generate Human human airway human respiratory virus human respiratory viruses immunoprophylaxis immunotherapies Infants Infection innate immune responses invasive lung organoid Model Mucus noninvasive technique nose nose organoids occurred Organoid organoids palivizumab Pathogenesis Patient predict Prevent prevented respiratory respiratory syncytial virus respiratory syncytial virus (RSV) respiratory viral respiratory virus RSV SARS-CoV-2 SARS-COV-2 infection severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Therapeutics unmet need viral infection viral shedding [DOI] 10.1128/mbio.03511-21 PMC 바로가기 [Article Type] Research Article
Topical TMPRSS2 inhibition prevents SARS-CoV-2 infection in differentiated human airway culturesResearch Article Published on 2022-02-022022-10-30 Journal: Life Science Alliance [Category] COVID-19, COVID19(2023년), MERS, SARS, [키워드] acute respiratory syndrome administration air–liquid interface airway angiotensin-converting enzyme-2 apical surface binding Bioavailability blockade blocked Camostat mesylate Cell circulation cleavage clinical trial compounds coronavirus COVID-19 Culture cultures demonstrated develop effective enzalutamide epithelial cell evaluated experiment had no human airway Infection Local metabolised Prevent primary human airway Prophylaxis receptor restrict Safe SARS-CoV-2 SARS-CoV-2 entry SARS-COV-2 infection serine protease serine protease inhibitor Spike protein targeting therapeutic therapeutic strategy TMPRSS2 TMPRSS2 inhibitor topical transmembrane serine protease Treatment Viral transmission [DOI] 10.26508/lsa.202101116 PMC 바로가기 [Article Type] Research Article