Structural insights into the binding of SARS-CoV-2, SARS-CoV, and hCoV-NL63 spike receptor-binding domain to horse ACE2Article Published on 2022-10-062022-11-16 Journal: Structure (London, England : 1993) [Category] COVID19(2023년), SARS, 변종, [키워드] ACE2 acute respiratory syndrome affinity Analysis B.1.617.2 Beta bind binding binding affinity cell entry complex complex structure coronavirus Coronaviruses Delta Efficiency equine Equus functional Gamma hACE2 HCoV-NL63 HeLa cells high affinity horse human ACE2 indicated Infection infection assay molecular basis Mutation provide pseudovirus pseudovirus infection RBDs receptor Receptor binding domain Receptor-binding domain SARS-CoV SARS-CoV-2 SARS-CoV-2 variant significantly increased spike receptor-binding domain the RBD the SARS-CoV-2 the spike protein Transmission zoonotic [DOI] 10.1016/j.str.2022.07.005 PMC 바로가기
Human surfactant protein D facilitates SARS-CoV-2 pseudotype binding and entry in DC-SIGN expressing cells, and downregulates spike protein induced inflammation인간 계면활성제 단백질 D는 DC-SIGN 발현 세포에서 SARS-CoV-2 유사형 결합 및 진입을 촉진하고 스파이크 단백질 유도 염증을 하향 조절합니다Article Published on 2022-07-282022-09-11 Journal: Frontiers in Immunology [Category] COVID19(2023년), SARS, 유전자 메커니즘, 치료기술, [키워드] ACE2 Analysis Anti-viral binding carbohydrate cause Cell cell interaction cell line cell surface receptor cells chemokine chemokines collectins controls COVID-19 cytokine cytokine response Cytokines DC-SIGN dendritic cell domain downregulate downregulated eight epithelial expressing facilitate fragment of HEK 293T HEK293T cell HEK293T cells help Human IFN-α IL- 6 IL-1β IL-8 immune function immune response immunomodulatory effect increase independent independent of infection assay infection assays Inflammation inhibit SARS-CoV-2 innate immune innate immune system Interaction intercellular involved Macrophage macrophages MHC class MHC class II modulating mRNA expression mRNA level mRNA levels N-terminal naïve T cell naïve T cells NF-κB pathogen Pathogens pro-inflammatory cytokine pro-inflammatory cytokines Protein pseudotype RANTES receptors Replication rfhSP-D. RNA virus RT-qPCR SARS- CoV-2 SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 replication SARS-CoV-2 spike protein shown SIGN spike Spike protein surface receptor T cells the Spike THP-1 TNF-α treated Treatment Vero Cell Vero cells viral entry viruses [DOI] 10.3389/fimmu.2022.960733 PMC 바로가기 [Article Type] Article
Peptide-Antibody Fusions Engineered by Phage Display Exhibit an Ultrapotent and Broad Neutralization of SARS-CoV-2 Variants파지 디스플레이가 조작한 펩티드-항체 융합은 SARS-CoV-2 변이체의 초강력 및 광범위한 중화를 나타냅니다Article Published on 2022-07-152022-09-11 Journal: ACS chemical biology [Category] COVID19(2023년), SARS, 변종, 진단, [키워드] affinities affinity approach approved B.1.1.529 Bivalent bivalent IgG breadth clinically Coverage COVID-19 demonstrated display domain dominant ENhance exacerbated fused fusion fusions generate implicated IMPROVE Infection infection assay low potency Mutation N-terminal domain N-terminus NAb NAbs naïve neutralization neutralization potency neutralize neutralizing antibody NTD omicron peptide peptides phage RBD RBD mutation RBD mutations S-protein SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2 variants Spike protein spread of COVID-19 tetravalent the RBD the spike protein therapeutic Therapeutics trimer variant variants variants of concern vulnerability [DOI] 10.1021/acschembio.2c00411 PMC 바로가기 [Article Type] Article
Evaluation of the clinical evolution and transmission of SARS-CoV-2 infection in cats by simulating natural routes of infectionOriginal Article Published on 2022-03-032022-10-28 Journal: Veterinary Research Communications [Category] COVID-19, MERS, SARS, [키워드] acute respiratory syndrome affected Air renovation cats clinical evolution condition Contact coronavirus cough COVID-19 demonstrated evaluated expected experiment feature histological humans Infection infection assay lesion naïve natural infection organs outcome pandemic disease reported risk of infection Routes of infection SARS-CoV-2 sneeze subsequent supplementary material susceptibility susceptible Transmission transmission of SARS-CoV-2 Ventilation virus [DOI] 10.1007/s11259-022-09908-5 PMC 바로가기 [Article Type] Original Article
Determination of infectious hepatitis B virus particles by an end-point dilution assay identifies a novel class of inhibitorsArticle Published on 2021-12-012023-06-12 Journal: Antiviral Research [Category] B형 간염, [키워드] Hepatitis B virus immunofluorescence analysis infection assay Infectious virus particle multiplicity of infection Novel antivirals quantitative PCR Specific infectivity Tissue culture infectious dose 50. [DOI] 10.1016/j.antiviral.2021.105195
An Intranasal Vaccine Based on Outer Membrane Vesicles Against SARS-CoV-2Microbiology Published on 2021-11-052022-10-31 Journal: Frontiers in Microbiology [Category] COVID-19, [키워드] Vibrio cholerae adenoviral vector administration Against Antibody titer Antigen Bacterial component criteria Culture detectable Donor effective enterotoxigenic enterotoxigenic Escherichia coli Escherichia coli expression Heterologous highlight immune response immunization infection assay lentivirus limitation mRNA neutralization activity offered OMV outer member vesicles (OMV) outer membrane outer membrane vesicles pandemic Pathogens Protein RBD Receptor binding domain regimen robust Safe SARS-CoV-2 SARS-CoV-2 neutralizing antibodies SARS-CoV-2 vaccine spike Spike protein stability the SARS-CoV-2 the Spike turn V. cholerae Vaccine Vesicle Vibrio cholerae [DOI] 10.3389/fmicb.2021.752739 PMC 바로가기 [Article Type] Microbiology
Combination of a Sindbis-SARS-CoV-2 Spike Vaccine and αOX40 Antibody Elicits Protective Immunity Against SARS-CoV-2 Induced Disease and Potentiates Long-Term SARS-CoV-2-Specific Humoral and T-Cell Immunitysindbis-sars-cov-2 스파이크 백신과 αOX40 항체의 조합은 SARS-COV-2 유도 질환에 대한 보호 면역을 유도하고 장기 SARS-COV-2- 특이 적 유머 및 T- 세포 면역을 강화시킨다.Immunology Published on 2021-07-292022-08-31 Journal: Frontiers in Immunology [Category] SARS, 바이오마커, 변종, 진단, [키워드] αOX40 activated T cells Against alphavirus vaccine Analysis antibody approach can be used caused cellular coronavirus coronavirus SARS-CoV-2 COVID-19 pandemic COVID19 develop dose effective effective vaccine effector memory effort elicit elicited expressing functions generate hACE2 human ACE2 receptor immune protection immune response immunization immunize Immunophenotyping immunostimulatory induced Infection infection assay inhibit long-lasting neutralizing antibodies long-lasting neutralizing antibody lung tissue mice MOST mounted Neutralizing antibodies OX40 Pathogens platform profiles protective immunity Protein binding provided reprogramming RNA robust SARS-CoV-2 SARS-CoV-2 immunity SARS-CoV-2 spike protein SARS-CoV-2 vaccine sera Sindbis virus vaccine spike Spike protein synergistic combination SARS-CoV-2 vaccine strategy T cell T cells T-cell Response T-cell responses the SARS-CoV-2 the vaccine Transcriptome transgenic mice Vaccine vaccine platform variant variants [DOI] 10.3389/fimmu.2021.719077 PMC 바로가기 [Article Type] Immunology
ACE2 receptor usage reveals variation in susceptibility to SARS-CoV and SARS-CoV-2 infection among bat species Research Published on 2021-03-012022-10-05 Journal: Nature Ecology & Evolution [Category] SARS, 변종, 유전자 메커니즘, [키워드] ACE2 ACE2 receptor acute respiratory syndrome Analysis angiotensin-converting enzyme 2 bat bat ACE2 both virus both viruses cell lines Contact coronavirus coronavirus disease COVID-19 Critical Cross-species transmission functional Genetic Host host receptor Human human cell humans identify Infection infection assay Interaction knowledge no correlation orthologue pandemic phylogenetically Probability receptor Receptor binding residue reveal SARS-CoV SARS-CoV-2 SARS-COV-2 infection suggested Support susceptibility Variation viral entry viral spike protein [DOI] 10.1038/s41559-021-01407-1 [Article Type] Research
Generation of SARS-CoV-2 Spike Pseudotyped Virus for Viral Entry and Neutralization Assays: A 1-Week ProtocolCardiovascular Medicine Published on 2021-01-152022-10-31 Journal: Frontiers in Cardiovascular Medicine [Category] COVID-19, [키워드] 293T ACE2 addition adjuvants African approaches caused Cell cell line cells clinically coronavirus COVID-19 COVID-19 pandemic ENhance epithelial furin Generation GFP glycoprotein green high risk human ACE2 receptor human Calu-3 Infection infection assay knowledge Laboratory lack lentivirus lung epithelial mechanism Monkey neutralizing antibody non-human primate outcome over-expression overexpression Patient performed PGE2 poloxamer 407 producing prostaglandin E2 protease protocol pseudotyped viral particle pseudovirus Quantitative renal SARS-CoV-2 SARS-COV-2 infection spike the SARS-CoV-2 therapeutic Therapeutic approach this system TMPRSS2 VERO-E6 vesicular stomatitis virus viral entry viral infection virus VSV VSVΔG [DOI] 10.3389/fcvm.2020.618651 PMC 바로가기 [Article Type] Cardiovascular Medicine
Validation and clinical evaluation of a SARS-CoV-2 surrogate virus neutralisation test (sVNT)Research Article Published on 2020-11-012022-10-31 Journal: Emerging Microbes & Infections [Category] COVID-19, MERS, SARS, [키워드] analytical sensitivity anti-SARS-CoV-2 antibody biosafety can be used cell-based virus neutralisation assay clinical clinical evaluation clinical sensitivity collected COVID-19 described detect determine Diagnosis ELISA functional Immune status increase in independent infection assay natural infection neutralisation neutralisation assay neutralisation assays Neutralising Antibodies PCR-confirmed COVID-19 performed positive Pseudotyped viruses pseudovirus neutralisation assay Receptor binding domain SARS-CoV-2 SARS-CoV-2 immunity SARS-CoV-2 neutralising antibody sensitivity sera severity specificity surrogate virus neutralisation assay sVNT symptom onset titre vaccinated individual vaccination validation Virus neutralisation virus neutralising assay wild type [DOI] 10.1080/22221751.2020.1835448 PMC 바로가기 [Article Type] Research Article