Insights into the Pathogenesis and Development of Recombinant Japanese Encephalitis Virus Genotype 3 as a VaccineArticle Published on 2024-05-302024-09-05 Journal: Vaccines [Category] update2024, [키워드] cDNA infectious clone Japanese encephalitis virus 3 Nakayama [DOI] 10.3390/vaccines12060597 PMC 바로가기 [Article Type] Article
Expanding the Hepatitis E Virus Toolbox: Selectable Replicons and Recombinant Reporter GenomesReview Published on 2023-03-282023-06-12 Journal: Viruses [Category] E형 간염, Fulltext, [키워드] epitope tag infectious clone insertion reporter selection gene subgenomic replicon. [DOI] 10.3390/v15040869 PMC 바로가기 [Article Type] Review
A Rapid Method for Generating Infectious SARS-CoV-2 and Variants Using Mutagenesis and Circular Polymerase Extension Cloningarticle Published on 2023-03-062024-09-05 Journal: Microbiology Spectrum [Category] update2024, [키워드] CPEC infectious clone Mutagenesis reverse genetics SARS-CoV-2 [DOI] 10.1128/spectrum.03385-22 PMC 바로가기 [Article Type] article
Short ‘1.2× Genome’ Infectious Clone Initiates Kolmiovirid Replication in Boa constrictor CellsArticle Published on 2022-01-082023-06-02 Journal: Viruses [Category] B형 간염, [키워드] deltavirus HDV infectious clone [DOI] 10.3390/v14010107 PMC 바로가기 [Article Type] Article
Crucial mutation in the exoribonuclease domain of nsp14 of PEDV leads to high genetic instability during viral replicationResearch Published on 2021-06-072022-11-01 Journal: Cell & Bioscience [Category] COVID-19, [키워드] active site acute infection acute respiratory syndrome Analysis Betacoronavirus Clinical signs clone contributing to coronavirus CoV Critical deficiency diarrhea died Disabled domain drugs epidemic diarrhea evaluate Exoribonuclease fecal functional Genetic genetics gnotobiotic in viral in vitro infectious clone inoculated investigated less MHV Middle East moderate murine hepatitis virus mutant mutants Mutation nonstructural protein 14 nsp14 nsp14-ExoN Pathogenesis PEDV pig plaque forming unit Porcine epidemic diarrhea virus predicted Proofreading replication fidelity reported rescued responsible Result reverse genetics Sanger sequencing SARS-CoV-2 sensitivity supplementary material syndrome TCID variant Vero cells viral genome viral replication Viral RNA virulent virus while Zinc [DOI] 10.1186/s13578-021-00598-1 PMC 바로가기 [Article Type] Research
Establishment of a reverse genetics system for SARS-CoV-2 using circular polymerase extension reaction순환중합효소연장반응을 이용한 SARS-CoV-2 역유전학 시스템 구축Article Published on 2021-04-202022-09-11 Journal: Cell Reports [Category] SARS, 변종, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus Analysis assembly bacterium base pairs causative agent cells clone construction coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 CPER DNA fragment extension functional generate genetics Genome high accuracy infectious clone lack multiple mutation multiple mutations Mutagenesis mutant virus mutant viruses Mutation PCR polymerase recombinant reporter reporter viruses rescued resulting reverse genetics SARS-CoV-2 SARS-CoV-2 cDNA SARS-CoV-2 infectious clones severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 susceptible transfection virus [DOI] 10.1016/j.celrep.2021.109014 PMC 바로가기 [Article Type] Article
Generation of Recombinant SARS-CoV-2 Using a Bacterial Artificial ChromosomeArticle Published on 2020-12-012023-07-13 Journal: Curr Protoc Microbiol [Category] SARS, [키워드] bacterial artificial chromosome coronavirus COVID-19 infectious clone reverse genetics SARS-CoV-2 virus rescue. [DOI] 10.1002/cpmc.126 PMC 바로가기
SARS-CoV-2 Reverse Genetics Reveals a Variable Infection Gradient in the Respiratory TractArticle Published on 2020-05-272023-06-15 Journal: Cell [Category] MERS, SARS, [키워드] ACE2 COVID-19 infectious clone nasal infection Neutralization assay primary cells reporter virus respiratory tropism reverse genetics SARS-CoV-2 [DOI] 10.1016/j.cell.2020.05.042 PMC 바로가기 [Article Type] Article
The Characterization of Immunoprotection Induced by a cDNA Clone Derived from the Attenuated Taiwan Porcine Epidemic Diarrhea Virus Pintung 52 StrainArticle Published on 2018-10-042022-10-28 Journal: Viruses [Category] Coronavirus, [키워드] antigenicity attenuation cDNA characterization Clinical signs clone comparable detectable determinant effective Effective vaccines elicited epidemic diarrhea facilitate fecal full-length cDNA Host immune response IgA immunogenic immunoprotection in vitro in vivo induced infectious clone inoculated less Modification neutralizing antibody titers parental PEDV plasma IgG replication kinetics reverse genetics strain Taiwan Vaccine vaccine platform Vero cells viral genome Viral load viral pathogenesis viral shedding virulent virus [DOI] 10.3390/v10100543 PMC 바로가기 [Article Type] Article
Infectivity of a genotype 4 hepatitis E virus cDNA clone by intrahepatic inoculation of laboratory ratsArticle Published on 2013-10-252023-06-11 Journal: Veterinary microbiology [Category] E형 간염, [키워드] days post-inoculation DPI genotype 4 hepatitis E virus Hepatitis E virus (HEV) HEV IFA immunofluorescence assay. infectious clone Rat animal model [DOI] 10.1016/j.vetmic.2013.06.021