An ISG15-Based High-Throughput Screening Assay for Identification and Characterization of SARS-CoV-2 Inhibitors Targeting Papain-like ProteaseArticle Published on 2024-08-012024-09-05 Journal: Viruses [Category] update2024, [키워드] FRET assay High-throughput screening ISG15 PLPro SARS-CoV-2 [DOI] 10.3390/v16081239 PMC 바로가기 [Article Type] Article
ISGylation of the SARS-CoV-2 N protein by HERC5 impedes N oligomerization and thereby viral RNA synthesisArticle Published on 2024-05-202024-09-05 Journal: bioRxiv [Category] update2024, [키워드] HERC5 ISG15 ISGylation N protein Papain-like protease SARS-CoV-2 [DOI] 10.1101/2024.05.15.594393 PMC 바로가기 [Article Type] Article
Case Report: ISG15 deficiency caused by novel variants in two families and effective treatment with Janus kinase inhibitionImmunology Case Report Published on 2023-12-052024-09-05 Journal: Frontiers in immunology [Category] update2024, [키워드] Baricitinib interferon Interferonopathy ISG15 ISG15 deficiency Janus kinase inhibition microdeletion whole exome sequencing [DOI] 10.3389/fimmu.2023.1287258 PMC 바로가기 [Article Type] Immunology Case Report
SARS-CoV-2 papain-like protease plays multiple roles in regulating cellular proteins in the endoplasmic reticulumarticle Published on 2023-10-122024-09-05 Journal: Brain stimulation [Category] update2024, [키워드] Cell Biology cholesterol COVID deubiquitinase deubiquitinating enzyme Deubiquitination Innate immunity interferon ISG15 lipid biosynthesis NSP proteolysis ubiquitin Virology virulence factor [DOI] 10.1016/j.jbc.2023.105346 PMC 바로가기 [Article Type] article
Dual domain recognition determines SARS-CoV-2 PLpro selectivity for human ISG15 and K48-linked di-ubiquitinArticle Published on 2023-01-192024-09-05 Journal: bioRxiv [Category] update2024, [키워드] COVID-19 crystal structure ISG15 PLPro SARS-CoV-2 ubiquitin [DOI] 10.1101/2021.09.15.460543 PMC 바로가기 [Article Type] Article
Coronaviral PLpro proteases and the immunomodulatory roles of conjugated versus free Interferon Stimulated Gene product-15 (ISG15)Review Published on 2022-12-012023-07-09 Journal: Seminars in cell & developmental biology [Category] COVID19(2023년), [키워드] coronavirus Deubiquitinating enzymes DUB HERC5 interferon ISG15 MERS-CoV papain-like proteases PLPro SARS-CoV SARS-CoV2 ubiquitin Ubiquitin E3 ligases Ubiquitin-Like proteins Ubiquitin-specific proteases. USP [DOI] 10.1016/j.semcdb.2022.06.005 PMC 바로가기 [Article Type] Review
More tools for our toolkit: The application of HEL-299 cells and dsRNA-nanoparticles to study human coronaviruses in vitroArticle Published on 2022-11-012022-11-15 Journal: Virus research [Category] SARS, 치료법, 치료제, [키워드] ACE2 receptor Analysis Antiviral antiviral genes antiviral response antiviral therapy can be used Cell cell line cells Combination competent Complete coronavirus Coronavirus replication CXCL10 cytidylic acid double-stranded expand fibroblast Gene Expression HCoV-229E HCoV-NL63 help Human human coronavirus human coronaviruses human lung IFN IFNs IFNβ in vitro increase in inducer Infection inhibit innate immune interferons ISG15 limit MERS-CoV Nanoparticles Pathogens Poly I:C. post infection PROTECT provided required SARS-CoV-2 SARS-CoV-2 pandemic stimulate translated Treatment Type I IFN type I interferon virus production were infected while [DOI] 10.1016/j.virusres.2022.198925 PMC 바로가기
Curcumin partly prevents ISG15 activation via ubiquitin-activating enzyme E1-like protein and decreases ISGylationArticle Published on 2022-10-152022-11-15 Journal: Biochemical and Biophysical Research Communication [Category] 치료제, [키워드] A549 cell activated Activation acute respiratory syndrome Affect and SARS-CoV-2 papain-like protease. contribute coronavirus coronavirus disease COVID-19 Curcumin decrease dose-dependent manner enzyme expression expression level function help immunomodulator Infection innate immune responses interferon interferon-stimulated gene Interferon-stimulated gene 15 kDa investigated ISG15 ISGylation kDa Modification monomer occur Papain-like protease pathogen Prevent Protein reduce SARS-CoV-2 severe COVID-19 Spread substrate suggested treated Treatment [DOI] 10.1016/j.bbrc.2022.08.003 PMC 바로가기
SARS-CoV-2 Papain-Like Protease: Structure, Function and InhibitionArticle Published on 2022-10-062022-11-15 Journal: Chembiochem [Category] COVID19(2023년), SARS, 변종, 치료제, [키워드] antiviral agent antiviral immune response antivirals coronavirus COVID-19 derivatives Epidemic function Host immune response immune immune response in viral inhibition involved ISG15 less MERS-CoV motif other coronaviruses Papain-like protease posttranslational modifications protease protease inhibitors Replication SARS-CoV-1 SARS-CoV-2 Structure targets variants of concern variants of SARS-CoV-2 Viral protein [DOI] 10.1002/cbic.202200327 PMC 바로가기
Expedient Synthesis of Ubiquitin-like Protein ISG15 Tools through Chemo-Enzymatic Ligation Catalyzed by a Viral Protease LbproArticle Published on 2022-10-042023-07-10 Journal: Angewandte Chemie (International ed. in English) [Category] COVID19(2023년), [키워드] Chemical Protein Synthesis ISG15 ligation ubiquitin Ubiquitin-Like Protein. [DOI] 10.1002/anie.202206205