Reverse transcriptase-free detection of viral RNA using Hemo Klentaq DNA polymeraseArticle Published on 2022-12-152022-11-15 Journal: Analytical biochemistry [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] affected amplification Analysis can be used caused comparable COVID-19 Degraded RNA demonstrated determine DNA polymerase Efficiency freeze-thaw cycles limitations Nasopharyngeal swab Nearby Nearby primers overcome pandemic patients PCR polymerase chain polymerase chain reaction Primers provide provided reliability reverse transcriptase Reverse transcriptase activity reverse transcription RNA RT-PCR SARS-CoV-2 SARS-CoV-2 detection target transcriptase Viral RNA [DOI] 10.1016/j.ab.2022.114960 PMC 바로가기
Development of a magnetic nanoparticle-based method for concentrating SARS-CoV-2 in wastewaterArticle Published on 2022-11-202022-11-15 Journal: The Science of the total environment [Category] COVID19(2023년), SARS, 치료기술, [키워드] acute respiratory syndrome affected applicability applied comparable Concentration coronavirus detect Detection sensitivity development Epidemiology limitations log magnetic nanoparticle Mild MNP Modification MS2 Pegcision Polyethylene glycol Polyethylene glycol precipitation polymerase chain Pseudomona quantified reducing SARS-CoV-2 SARS-CoV-2 RNA sensitivity virus virus concentration viruses Wastewater-based epidemiology [DOI] 10.1016/j.scitotenv.2022.157613 PMC 바로가기
Adsorption of SARS-CoV-2 onto granular activated carbon (GAC) in wastewater: Implications for improvements in passive samplingArticle Published on 2022-11-152022-11-15 Journal: The Science of the total environment [Category] COVID19(2023년), SARS, 치료기술, [키워드] activated acute respiratory syndrome Adsorption Adsorption capability anticipated approaches calculated Characteristics Concentration coronavirus DI water experiment feasible granular Granular activated carbon hybrid improvement interval Laboratory limitations occurred offer Passive Passive samplers potential benefit reached reproducibility RT-qPCR SARS-CoV-2 SARS-CoV-2 RNA sensitivity Surveillance viral pathogen virus wastewater wastewater surveillance [DOI] 10.1016/j.scitotenv.2022.157548 PMC 바로가기
Randomized, double-blind, placebo-controlled, crossover trial of oral doxycycline for epistaxis in hereditary hemorrhagic telangiectasiaArticle Published on 2022-11-072022-11-16 Journal: Orphanet Journal of Rare Diseases [Category] 유전자 메커니즘, [키워드] Analysis Angiogenesis assigned Biomarkers bleeding Blood center double-blind doxycycline Effectiveness ENG Epistaxis Frequency heart failure help hemorrhage Hemorrhagic Hereditary Hereditary hemorrhagic telangiectasia Hereditary hemorrhagic telangiectasia. HHT IL-6 Inflammatory intravenous limitations Liver disease malformation marker mechanism Michael morbidity no significant difference not significantly different observation pathway Patient Placebo placebo-controlled provided Quality of life Randomized controlled trial Randomly RBC recruited Registered Registration secondary significantly higher stroke telangiectasia therapy Toronto Treatment treatment period Trial VEGF [DOI] 10.1186/s13023-022-02539-8 PMC 바로가기
Fluorescent nanodiamond-based spin-enhanced lateral flow immunoassay for detection of SARS-CoV-2 nucleocapsid protein and spike protein from different variantsArticle Published on 2022-10-162022-11-15 Journal: Analytica chimica acta [Category] COVID19(2023년), SARS, 변종, 진단, 치료기술, [키워드] anti-S IgG antibody Antigen antigens B.1.1.529 B.1.1.7 B.1.617.2 binding can be used comparable COVID-19 COVID-19 pandemic current Delta demonstrated Detection limit Diagnosis diagnostic Direct dropped ELISA finding Flow fluorescent Fluorescent nanodiamond incubated Infectious disease Laboratory Lateral lateral flow immunoassay limitations membrane mutations NC membrane nonspecific omicron platform Protein public health Quantitative reporter responsible S proteins SARS-CoV-2 SARS-CoV-2 antibody SARS-CoV-2 antigen SARS-CoV-2 N protein SARS-CoV-2 nucleocapsid SELFIA. Spike protein suggested the SARS-CoV-2 turnaround times variant variants viruses was performed wild-type Wuhan-1 [DOI] 10.1016/j.aca.2022.340389 PMC 바로가기
Digital RT-PCR Chip method for detection of SARS-CoV-2 virusArticle Published on 2022-10-012022-11-15 Journal: Journal of Immunological Methods [Category] COVID19(2023년), SARS, 치료기술, [키워드] applied asymptomatic subject biological sample characterized ChIP Digital Digital RT-PCR limitations Nasopharyngeal swab PCR performed quantification real time recruited RT-PCR RT-qPCR SARS-CoV-2 SARS-CoV-2 viral load SARS-CoV-2 virus sensitivity spike the SARS-CoV-2 virus UNICORN Viral load [DOI] 10.1016/j.jim.2022.113339 PMC 바로가기
Validation of a duplex PCR technique using the gen E and RNase P for the diagnosis of SARS-CoV-2Article Published on 2022-10-012022-11-16 Journal: Enfermedades infecciosas y microbiologia clinica ( [Category] COVID19(2023년), SARS, 치료기술, [키워드] Analysis Berlin protocol Charité clinical samples COVID-19 COVID-19 diagnosis Cribado masivo Diagnosis diagnosis of SARS-CoV-2 Diagnóstico E gene English Faculty of Medicine Genes independent Laboratory limit of detection limitations mass Mass screening no significant difference not affect PCR PCR en tiempo real polymerase chain protocol reagent real time Real-time PCR reduce reliability reported reproducibility reverse transcriptase RNA sample RNase P RT-PCR SARS-CoV-2 sensitivity Sensitivity and specificity similarity Spanish specificity specimen threshold validation World Health Organization [DOI] 10.1016/j.eimce.2022.05.005 PMC 바로가기
Challenges in the Detection of SARS-CoV-2: Evolution of the Lateral Flow Immunoassay as a Valuable Tool for Viral DiagnosisArticle Published on 2022-09-052022-11-15 Journal: Biosensors [Category] SARS, 진단, 치료기술, [키워드] affected antibodies biosensors caused challenge coronavirus disease COVID-19 deaths detect detection Diagnosis diagnosis of SARS-CoV-2 diagnostic Evolution Flow Health immunoassay infected patient Infectious disease Lateral lateral flow immunoassay LFIA limitations outbreak pandemic SARS-CoV-2 Sensitivity and specificity Spread the SARS-CoV-2 triggered virus zoonotic origin [DOI] 10.3390/bios12090728 PMC 바로가기
Second round of an interlaboratory comparison of SARS-CoV2 molecular detection assays used by 45 veterinary diagnostic laboratories in the United StatesArticle Published on 2022-09-012022-11-15 Journal: Journal of veterinary diagnostic investigation : o [Category] SARS, 변종, 치료기술, [키워드] addition analyst analytical sensitivity Analytical specificity analyzed assays B.1.1.7 B.1.351 buffer COVID-19 COVID-19 pandemic Critical detect diagnosis of COVID-19 diagnostic evaluate evaluated FDA help humans identify interlaboratory comparison investigation Laboratory limitations marker medium molecular N1 and N2 network nucleocapsid pandemic public health real-time RT-PCR recognize response RNA RT-rtPCR SARS-CoV2 second specificity Support the United State Transport variant variants Viral RNA [DOI] 10.1177/10406387221115702 PMC 바로가기
Two-stage hybrid network for segmentation of COVID-19 pneumonia lesions in CT images: a multicenter studyCT 이미지에서 COVID-19 폐렴 병변 분할을 위한 2단계 하이브리드 네트워크: 다기관 연구Multicenter Study Published on 2022-09-012022-09-11 Journal: Medical & biological engineering & computing [Category] COVID19(2023년), SARS, 치료기술, [키워드] Accuracy approach Automatic automatically characteristic Chest computed tomography Computed tomography COVID-19 COVID-19 diagnosis COVID-19 pneumonia Diagnosis Dice evaluated GPU hospitals identify Infected lesion segmentation lesion limitations lung manifestation memory multicenter outbreak Pneumonia pneumonia lesion Prognosis radiologist Radiologists regions required Screening sensitivity Seven specificity two-dimensional [DOI] 10.1007/s11517-022-02619-8 PMC 바로가기 [Article Type] Multicenter Study