BDA-410 inhibits SARS-CoV-2 main protease activity and viral replication in mammalian cellsArticle Published on 2022-10-012022-11-15 Journal: Journal of Cellular and Molecular Medicine [Category] COVID19(2023년), SARS, 치료제, [키워드] Abstract BDA-410 Calpain-1 COVID-19 inhibit mammalian cell SARS-CoV-2 SARS-CoV-2 main protease SARS-CoV-2. viral replication [DOI] 10.1111/jcmm.17442 PMC 바로가기
SARS-CoV-2 impairs the disassembly of stress granules and promotes ALS-associated amyloid aggregationSARS-CoV-2는 스트레스 과립의 분해를 손상시키고 ALS 관련 아밀로이드 응집을 촉진합니다Article Published on 2022-08-012022-09-11 Journal: Protein & Cell [Category] COVID19(2023년), SARS, 치료법, [키워드] ACE2 addition aggregation bind Cell cells cellular clearance experiment FUS Host host cells impair in vitro incorporation increased risk indicate infect Infection interactions Invasion mammalian cell mammalian cells Mutation N protein neurodegeneration NMR non-specific nucleocapsid nucleocapsid protein occur phase transition promote Protein Proteins reported SARS-CoV-2 SARS-COV-2 infection scenario stimulated Stress stress granule stress granule. stress granules [DOI] 10.1007/s13238-022-00905-7 PMC 바로가기 [Article Type] Article
RelCoVax®, a two antigen subunit protein vaccine candidate against SARS-CoV-2 induces strong immune responses in miceSARS-CoV-2에 대한 2개의 항원 소단위 단백질 백신 후보인 RelCoVax®는 마우스에서 강력한 면역 반응을 유도합니다Article Published on 2022-07-302022-09-11 Journal: Vaccine [Category] COVID19(2023년), MERS, SARS, 변종, 진단, 치료법, [키워드] adjuvant adjuvants Administered alum aluminum aluminum hydroxide Antibody titer Antibody titers Antigen antigen exposure Cell Cellular immune response cellular immune responses Combination contribute COVID-19 pandemic CpG Delta delta variant demonstrated develop dominant dose regimen dual E. coli effective vaccine elicited Emergency use authorization evade evidenced glycoprotein heterologous expression hydroxide IFN-γ immune responses immunize immunized immunized mice in vitro induce mammalian cell mammalian cells mice Mutation N protein N proteins neutralize Neutralizing antibodies neutralizing antibody nucleocapsid prime and boost Protein RBD Receptor binding domain Region RelCoVax® S protein Safe SARS-CoV-2 SARS-CoV-2 viral SARS-CoV-2 virus serum splenocyte splenocytes stimulated strain strong immune response subunit Subunit vaccine Subunit vaccine. Th1 the SARS-CoV-2 virus vaccinated individuals Vaccine vaccine candidate vaccine candidates variants of SARS-CoV-2 viral strains virus entry [DOI] 10.1016/j.vaccine.2022.06.026 PMC 바로가기 [Article Type] Article
Comparative evaluation of flavonoids reveals the superiority and promising inhibition activity of silibinin against SARS-CoV-2플라보노이드의 비교 평가는 SARS-CoV-2에 대한 silibinin의 우수성과 유망한 억제 활성을 나타냅니다Article Published on 2022-07-012022-09-11 Journal: Phytotherapy research : PTR [Category] COVID19(2023년), SARS, 치료제, [키워드] amino acids antiviral activity binding binding affinity Comparative Compound enzyme Enzyme inhibition evaluated exhibited FDA-approved drug flavonoid flavonoids followed by highest highest binding affinity in silico in silico study in vitro in vitro evaluation in vitro study indicated inhibition activity inhibition assay Interaction interfere key amino acid key amino acids M pro mammalian cell mapping molecular docking plant plants protease Protein RdRP Replication reveal S protein Safe SARS-CoV-2 SARS-CoV-2 entry SARS-CoV-2 protein SARS-CoV-2 S protein Screening selectivity index Serine significantly silibinin silibinin. systematic literature search targets TMPRSS2 transmembrane serine protease virucidal was tested [DOI] 10.1002/ptr.7486 PMC 바로가기 [Article Type] Article
Pseudomonas aeruginosa: pathogenesis, virulence factors, antibiotic resistance, interaction with host, technology advances and emerging therapeutics녹농균(Pseudomonas aeruginosa): 병인, 독성 인자, 항생제 내성, 숙주와의 상호 작용, 기술 발전 및 새로운 치료법Review Published on 2022-06-252022-09-11 Journal: Signal Transduction and Targeted Therapy [Category] Fulltext, MERS, 유전자 메커니즘, [키워드] addition Antibiotic resistance Antibiotics antimicrobial autophagy Bacterial Bacterial pathogenesis bioinformatics burn wounds Cancer caused Cell signaling cGAS chronic obstructive clinically COPD COVID-19 CRISPR CRISPR-Ca cystic fibrosis Defense develop disorder drug drug resistance Drug screening effective effort Factor Factors fibrosis gram Host host defense host immune responses host-pathogen interaction immune immune responses immunodeficiency IMPROVE infect Infection infections Inflammasome Interaction intractable invasive LPS mammalian cell mechanism metabolomics molecular Non-coding RNAs opportunistic pathogen outer membrane P. aeruginosa pathogen Pathogenesis Pathways Patient platform Pseudomona Pseudomonas aeruginosa rapid progress Regulation Research scRNA-seq secretion Severe infection signaling pathways Strains therapy traits treat use of antibiotics Ventilation Vesicle virulence widespread [DOI] 10.1038/s41392-022-01056-1 PMC 바로가기 [Article Type] Review
Immunological investigation of a multiepitope peptide vaccine candidate based on main proteins of SARS-CoV-2 pathogenSARS-CoV-2 병원체의 주요 단백질에 기반한 다중 에피토프 펩타이드 백신 후보의 면역학적 조사Article Published on 2022-06-092022-09-11 Journal: PLoS ONE [Category] COVID19(2023년), SARS, 변종, 치료법, [키워드] antigenic Cell complex cytotoxic T lymphocyte develop DNA E. coli Epitopes eukaryotic evaluated expression expression vectors granzyme granzyme B groups helper T lymphocyte Heterologous homologous IFN-γ IgG IgG2a IL-15 IL-21 IL-6 immune response immune responses Immunity immunized in silico in vitro in vivo indicated induce linear B linear B lymphocyte lymphocyte mammalian cell membrane protein mice multiepitope peptide vaccine multiepitope vaccine Multiepitope vaccines nucleocapsid Nucleocapsid phosphoprotein pathogen Pathogens pcDNA3.1 Penetrating peptide produced Protein regimen regimens SARS-CoV-2 SARS-COV-2 infection secretion significantly spike glycoprotein stimulated subcloned T lymphocyte target targets TNF-α Vaccine vaccine candidate variants were used [DOI] 10.1371/journal.pone.0268251 PMC 바로가기 [Article Type] Article
Discovery and Use of Long dsRNA Mediated RNA Interference to Stimulate Antiviral Protection in Interferon Competent Mammalian Cells인터페론 유능한 포유동물 세포에서 항바이러스 보호를 자극하기 위한 긴 dsRNA 매개 RNA 간섭의 발견 및 사용Article Published on 2022-05-062022-09-11 Journal: Frontiers in Immunology [Category] COVID19(2023년), SARS, 치료법, 치료제, [키워드] Antiviral Antiviral effect antiviral response antiviral responses cell line cells cellular cleaved comparable competent complementary Concentration cornerstone CoV dependent on Dicer dsRNA Evidence explanation expressing fluorescent functional gene targeting generate green fluorescent protein HCoV HCoV-229E human coronavirus human coronaviruses IFN immune defense induce inducer inhibit Interference interferons knockdown Lineage Long long dsRNA mammalian mammalian cell mammalian cells Mediated mRNA pathway platform preventing infection produced protection Protein provide required responsible retained RISC RNA RNA interference RNA-induced silencing complex RNAi RNAi pathway SARS-CoV-2 replication sequence shown significantly siRNA siRNAs stimulate stomatitis suppress therapeutic These cells threshold type I interferon vesicular stomatitis vesicular stomatitis virus viral inhibition. virus VSV-GFP [DOI] 10.3389/fimmu.2022.859749 PMC 바로가기 [Article Type] Article
Designed SARS-CoV-2 receptor binding domain variants form stable monomers설계된 SARS-CoV-2 수용체 결합 도메인 변이체가 안정적인 단량체를 형성합니다.Article Published on 2022-05-012022-09-11 Journal: Biotechnology Journal [Category] COVID19(2023년), MERS, SARS, 변종, 진단, 치료기술, 치료제, [키워드] addressed aggregates antibody antibody assay validation Antigen antigen stability binding domain Cell convalescent sera COVID-19 COVID-19 patient cysteine cysteine residue Deletion enzyme enzyme-linked immunosorbent assay enzyme-linked immunosorbent assays eukaryotic systems expression HEK293 homogeneous intrinsic maintenance mammalian cell mammalian cells monomer monomeric Neutralizing antibodies O-glycosylation site produced Protein RBD RBD variant Receptor binding Receptor binding domain recombinant expression. reduce SARS-CoV-2 antibodies SARS-CoV-2 antibody SARS-CoV-2 receptor SARS-CoV-2 spike SARS-CoV-2 test sequence serological shown Substitution the SARS-CoV-2 vaccine candidates Vaccine development variant virus-neutralizing antibodies virus-neutralizing antibody [DOI] 10.1002/biot.202100422 PMC 바로가기 [Article Type] Article
Phosphate-regulated expression of the SARS-CoV-2 receptor-binding domain in the diatom Phaeodactylum tricornutum for pandemic diagnostics유행성 진단을 위한 규조류 Phaeodactylum tricornutum에서 SARS-CoV-2 수용체 결합 도메인의 인산염 조절 발현Article Published on 2022-04-292022-09-11 Journal: Scientific Reports [Category] COVID19(2023년), SARS, 변종, 진단, 치료기술, [키워드] alleviate anti-RBD antibiotic Antigen approach Betacoronavirus binding Biomedical research C-terminal can be used caused Cell coronavirus COVID-19 pandemic cross-reactive detect develop diagnostics domain Donor eukaryotic expressed expression expression plasmids finding glycosylated growth media histidine human ACE2 receptor inhibited iron lateral flow mammalian cell overexpression pandemic performed phosphate Plasmid polyclonal antibodies promoter purified RBD Receptor-binding domain retained SARS-CoV-2 SARS-CoV-2 proteins SARS-CoV-2 spike protein sensitivity serum specific IgG antibodies specific IgG antibody Spike protein Synthetic biology TEV protease the RBD the receptor-binding domain the SARS-CoV-2 Therapeutics Treatment type strain Viral antigen wild-type strain yield [DOI] 10.1038/s41598-022-11053-7 PMC 바로가기 [Article Type] Article
Sonogenetic control of mammalian cells using exogenous Transient Receptor Potential A1 channels Research Published on 2022-02-092022-10-05 Journal: Nature Communications [Category] 변종, [키워드] mammalian cell Potential Transient [DOI] 10.1038/s41467-022-28205-y [Article Type] Research