Simultaneous Detection of Porcine Respiratory Coronavirus, Porcine Reproductive and Respiratory Syndrome Virus, Swine Influenza Virus, and Pseudorabies Virus via Quadruplex One-Step RT-qPCRArticle Published on 2024-04-192024-09-05 Journal: Pathogens [Category] update2024, [키워드] Detection method multiplex RT-qPCR Porcine reproductive and respiratory syndrome virus (PRRSV) porcine respiratory coronavirus (PRCoV) pseudorabies virus (PRV) swine influenza virus (SIV) [DOI] 10.3390/pathogens13040341 PMC 바로가기 [Article Type] Article
Development of a robust TaqMan probe-based one-step multiplex RT-qPCR for simultaneous detection of SARS-CoV-2 and Influenza A/B virusesarticle Published on 2023-11-112024-09-05 Journal: BMC Microbiology [Category] update2024, [키워드] Co-infection influenza A virus influenza B virus multiplex RT-qPCR SARS-CoV-2 [DOI] 10.1186/s12866-023-03048-9 PMC 바로가기 [Article Type] article
Emergence of swine influenza A virus, porcine respirovirus 1 and swine orthopneumovirus in porcine respiratory disease in Germanyarticle Published on 2023-08-022024-09-05 Journal: Emerging Microbes & Infections [Category] update2024, [키워드] emerging viruses multiplex RT-qPCR porcine respirovirus 1 Surveillance swine influenza A virus swine orthopneumovirus zoonosis [DOI] 10.1080/22221751.2023.2239938 PMC 바로가기 [Article Type] article
Variant-specific RT-qPCR for rapid screening of B.1.617 mutations in SARS-CoV-2Article Published on 2022-12-012022-11-15 Journal: The Libyan journal of medicine [Category] COVID19(2023년), SARS, 변종, 치료기술, [키워드] B.1.617 B.1.617 lineage B.1.617.1 B.1.617.2 collected country Delta delta variant detect Diagnostic method evaluate kappa variant. L452R Libya Lineage multiplex RT-qPCR Mutation mutations new SARS-CoV-2 P681 PCR assay positive sample required RT- qPCR RT-qPCR RT-qPCR assay SARS-CoV-2 screened Sequencing Spread tested variant virus whole genome [DOI] 10.1080/19932820.2022.2121252 PMC 바로가기
Performance evaluation of a non-invasive one-step multiplex RT-qPCR assay for detection of SARS-CoV-2 direct from saliva타액에서 직접 SARS-CoV-2 검출을 위한 비침습적 원스텝 멀티플렉스 RT-qPCR 분석의 성능 평가Article Published on 2022-07-072022-09-11 Journal: Scientific Reports [Category] COVID19(2023년), SARS, 변종, 진단, 치료기술, [키워드] accreditation administer approach bypasse bypasses Chain Reaction clinical settings Compliance Concordance criteria demonstrated diagnostic diagnostic testing evaluated followed by helping increased risk invasive medium multiplex RT-qPCR nasopharyngeal specimen Non-invasive overcome Patient PCR performance performed polymerase chain reaction protocol qPCR assay quantitative PCR reducing Regulatory resulting reverse transcription RNA extraction robust RT-qPCR Saliva saliva specimens sample collection SARS-CoV-2 SARS-CoV-2 detection sensitivity service Swab swabbing swabs Testing United Kingdom university [DOI] 10.1038/s41598-022-15616-6 PMC 바로가기 [Article Type] Article
Efficient SARS-CoV-2 Quantitative Reverse Transcriptase PCR Saliva Diagnostic Strategy utilizing Open-Source Pipetting Robots오픈 소스 피펫팅 로봇을 활용한 효율적인 SARS-CoV-2 정량적 역전사효소 PCR 타액 진단 전략Article Published on 2022-02-112022-09-11 Journal: Journal of visualized experiments : JoVE [Category] COVID19(2023년), SARS, 치료기술, [키워드] automated Automation average Chain Reaction Clemson University clinical testing Community comparable complex Concentration COVID-19 pandemic diagnostic diagnostic testing efficient epidemiological handling Health Health crisis introduced invasive less Mortality multiplex RT-qPCR nasopharyngeal swabs nucleic acid nucleic acid testing Participants PCR physically polymerase chain polymerase chain reaction Population population surveillance Quantitative reagent Reporting Research reverse transcriptase robot RT-qPCR Saliva SARS-CoV-2 Sensitivity and specificity shown Strategy Supply chain Surveillance thermocycler thermocyclers transcriptase Transmission Viral RNA [DOI] 10.3791/63395 PMC 바로가기 [Article Type] Article
Diagnostic performance and clinical feasibility of a novel one-step RT-qPCR assay for simultaneous detection of multiple severe acute respiratory syndrome coronaviruses다중 심각한 급성 호흡기 증후군 코로나 바이러스의 동시 검출을위한 새로운 1 단계 RT-QPCR 분석의 진단 성능 및 임상 적 타당성Original Article Published on 2022-02-092022-09-10 Journal: Archives of Virology [Category] COVID19(2023년), Fulltext, MERS, SARS, 변종, 치료기술, 치료제, [키워드] Acute respiratory infection acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Analysis can be used caused cell culture supernatants Chain Reaction circulating variant clinical outcomes clinical sample coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 CoVs Critical cross-reaction Diagnosis early diagnosis evaluated event feasibility genes encoding healthy individuals human CoV human CoVs humans IMPROVE in vitro infect Laboratory laboratory diagnosis limit of detection majority membrane mild respiratory symptoms mRNA multiplex RT-qPCR nasal swab Other other coronaviruses Other respiratory viruses patients with COVID-19 polymerase chain polymerase chain reaction Protein qPCR assay Quantitative reaction respiratory respiratory infection Respiratory samples respiratory symptoms respiratory viruses reverse transcription RNA RT-qPCR SARS-CoV-2 SARS-like CoV SARS-like CoVs SARS-related CoV severe acute respiratory syndrome Coronavirus sputum samples supplementary material TCID Transmission zoonotic origin [DOI] 10.1007/s00705-022-05383-0 PMC 바로가기 [Article Type] Original Article
Production and use of encapsidated RNA mimics as positive control reagents for SARS-CoV-2 RT-qPCR diagnosticsArticle Published on 2022-02-012022-10-30 Journal: Journal of virological methods [Category] COVID-19, COVID19(2023년), MERS, SARS, [키워드] American CDC Berlin can be used Charité Chinese CDC Combination Control COVID-19 Cowpea mosaic virus Diagnosis diagnostics Efficiency Genome gold standard hydrolysis in vivo In-tube control MONITOR multiplex RT-qPCR Multiplexing Nicotiana benthamiana novel nucleic acid extraction packaged Particle Patient PCR amplification plant virus polymerase chain positive control produced Production protocols Pseudogenome purified Reactions reagent reverse transcription RNA RNA extraction RT-qPCR SARS-CoV-2 sequence Specific swab sample transient expression virus virus particle Virus-like particle virus-like particles VLP [DOI] 10.1016/j.jviromet.2021.114372 PMC 바로가기 [Article Type] Article
Diagnostic Performance of Automated SARS-CoV-2 Antigen Assay in Nasal Swab during COVID-19 Vaccination CampaignArticle Published on 2021-11-152022-10-31 Journal: Diagnostics [Category] COVID-19, [키워드] 95% CI acute respiratory syndrome Ag-RDT applied automated chemiluminescence immunoassay collected coronavirus COVID-19 COVID-19 diagnosis cut-off cycle threshold diagnose Diagnosis diagnostic DiaSorin evaluated identify individuals Infection Laboratory LIAISON median moderate multiplex RT-qPCR Nasopharyngeal swab pandemic Participants performance Prospective Study Quantitative Randomly rapid decisions RT-PCR RT-qPCR SARS-CoV-2 SARS-CoV-2 antigen SARS-COV-2 infection SARS-CoV-2-infected individual SARS-CoV2 sensitivity Sensitivity and specificity Spread subjects Taking Viral load viral loads was increased was performed [DOI] 10.3390/diagnostics11112110 PMC 바로가기 [Article Type] Article
OmniSARS2: A Highly Sensitive and Specific RT-qPCR-Based COVID-19 Diagnostic Method Designed to Withstand SARS-CoV-2 Lineage EvolutionArticle Published on 2021-09-262022-10-28 Journal: Biomedicines [Category] COVID-19, [키워드] Accuracy affected analytical sensitivity B.1 B.1.1 B.1.1.7 B.1.177 clinical sample Comparative genomics conserved Coronaviridae coronavirus COVID-19 COVID-19 pandemic cross-reactivity detect diagnostic methods diagnostics E gene Evolution Extensive Genome genomic highest human betacoronavirus increase in knowledge less Lineage lineages molecular multiplex RT-qPCR Mutation ORF1ab Pathogens performed Prevalence Probability Region RT-qPCR S gene SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2 lineage sensitive sensitivity Specific specificity Strains Taking the SARS-CoV-2 genome transmission of SARS-CoV-2 Viral detection virus viruses Volume while [DOI] 10.3390/biomedicines9101314 PMC 바로가기 [Article Type] Article