A fast and sensitive absolute quantification assay for the detection of SARS-CoV-2 peptides using parallel reaction monitoring mass spectrometry병렬 반응 모니터링 질량 분석을 사용한 SARS-CoV-2 펩타이드 검출을 위한 빠르고 민감한 절대 정량 분석Article Published on 2022-08-152022-09-11 Journal: Journal of proteomics [Category] COVID19(2023년), SARS, 치료기술, 치료법, 치료제, [키워드] Absolute quantification Clinical diagnosis clinics COVID-19 Ct values Early detection enrichment heat heat-inactivated high resolution high viral load investigated mass mass spectrometry Nasopharyngeal swab nasopharyngeal swab sample Orbitrap pandemic Patient peptide peptides positive positive patient positive patients PRM Protein quantification required RT-qPCR SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2 nucleocapsid SARS-CoV-2 peptide SARS-CoV-2. sensitivity speed Surveillance swab samples the disease Viral load Viral protein Viral proteins [DOI] 10.1016/j.jprot.2022.104664 PMC 바로가기 [Article Type] Article
Site-Specific Glycosylation Patterns of the SARS-CoV-2 Spike Protein Derived From Recombinant Protein and Viral WA1 and D614G StrainsChemistry Published on 2021-11-192022-10-31 Journal: Frontiers in Chemistry [Category] COVID-19, [키워드] ACE2 receptor analysis analyzed Cell cell substrate characterized complex core Critical D614G D614G variant defined domain downstream Eclipse Efficiency glycan glycan shield glycans glycosylated glycosylation glycosylation pattern HEK293 high-mannose Host Host–pathogen interaction hydrophilic in viral Infection Interaction less Local microheterogeneity monomer Mutation N-glycan N-glycosylation N-glycosylation site O-glycosylation Orbitrap pattern predicted produced Protein RBD recent recombinant Recombinant protein reported reveal S1 and S2 S1 domain SARS-CoV-2 SARS-CoV-2 spike SARS-CoV-2 spike protein spike Spike protein Spike proteins strain substrate the SARS-CoV-2 Vero Vero cells Vero E6 virus WA1 WA1 strain [DOI] 10.3389/fchem.2021.767448 PMC 바로가기 [Article Type] Chemistry
Quantitative proteomics of hamster lung tissues infected with SARS-CoV-2 reveal host factors having implication in the disease pathogenesis and severitySARS-CoV-2에 감염된 햄스터 폐 조직의 정량적 단백질체학은 질병 발병기전 및 중증도에 영향을 미치는 숙주 인자를 나타냅니다Article Published on 2021-07-012022-09-11 Journal: FASEB journal : official publication of the Federa [Category] SARS, 유전자 메커니즘, [키워드] acute infection acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addition alveolar damage Analysis carried clinical parameter Clinical parameters coagulation cascade complement convalescence coronavirus COVID-19 COVID-19 patient COVID-19 patients DAD differential expression differentially expressed differentially expressed proteins Diffuse alveolar damage effort enriched pathways evaluate expression Factor Ferroptosis groups hamster hamsters histopathological Host in vivo infected with SARS-CoV-2 involved isolates Local lung Lung function Lung pathology lung proteome lung tissue lung tissues mass spectrometry Mesocricetus Orbitrap Pathogenesis pathological change pathophysiological Pathway analysis platelet activation Post-infection progression protective role Protein proteomic proteomics Pulmonary Surfactant pulmonary surfactant. Quantitative reported resolved SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 pathogenesis SARS-CoV-2-infected hamster SARS-CoV-2-infected hamsters severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 severity SFTPB SFTPD significantly surfactant Syrian golden hamster Syrian Golden hamsters the disease tissue sample tissue samples [DOI] 10.1096/fj.202100431R PMC 바로가기 [Article Type] Article
A preliminary metabolites identification of a novel compound with β-adrenolytic activityArticle Published on 2021-05-292022-10-28 Journal: Pharmacological Reports [Category] COVID-19, [키워드] Abstract Aminopropan-2-ol compound Analysis API cytochrome P450 derivative Electrochemistry Faeces High-resolution identification in vitro in vivo intestine kidneys liver lung m/z mass spectrometry metabolised metabolism metabolite metabolites Orbitrap oxidative Phase I predicted renal renal clearance Result serum Structure Urine was done [DOI] 10.1007/s43440-021-00273-9 PMC 바로가기 [Article Type] Article