Longer amplicons provide better sensitivity for electrochemical sensing of viral nucleic acid in water samples using PCB electrodes더 긴 앰플리콘은 PCB 전극을 사용하여 물 샘플에서 바이러스 핵산의 전기화학적 감지에 더 나은 감도를 제공합니다.Article Published on 2022-05-252022-09-11 Journal: Scientific Reports [Category] COVID19(2023년), SARS, 치료기술, [키워드] Amplicon amplicons COVID-19 pandemic demonstrated detect determine DNA DNA fragment effort Formula gel gold gold standard Interaction Methylene Methylene blue Modification offer PCR PCR product PCR products prominence purification salt SARS-CoV-2 sensitivity significantly Surveillance text Viral load viral nucleic acid water [DOI] 10.1038/s41598-022-12818-w PMC 바로가기 [Article Type] Article
Preliminary study of coronavirus disease 2019 on pets in pandemic in Denpasar, Bali, IndonesiaResearch Article Published on 2021-11-262022-10-29 Journal: Veterinary World [Category] COVID-19, [키워드] acute respiratory syndrome assumed Asymptomatic caused clinically close contact collected coronavirus disease Coronavirus disease 2019 Coronavirus-2 correlation COVID-19 Critical epidemiological eyes genetic material hospital Hospitalization Hospitalized Host Human humans individual Indonesia infected individual Infection limit material nasopharyngeal sample pandemic Patient patients with COVID-19 PCR product pets positive processed required Respiratory disease respiratory droplet resulting RNA isolation RNA samples RT-PCR SARS-CoV-2 SARS-COV-2 infection Seven severe acute respiratory syndrome coronavirus-2 Spread Swab swab sample swab samples tested the disease threshold through contact Transmission transmitted understanding Viral detection virus wild animal World Health Organization zoonotic transmission [DOI] 10.14202/vetworld.2021.2979-2983 PMC 바로가기 [Article Type] Research Article
Single-Point Mutations in the N Gene of SARS-CoV-2 Adversely Impact Detection by a Commercial Dual Target Diagnostic AssaySARS-CoV-2의 N 유전자에 있는 단일점 돌연변이는 상업용 이중 표적 진단 분석에 의한 역효과 검출Research Article Published on 2021-11-172022-09-10 Journal: Microbiology Spectrum [Category] MERS, SARS, 변종, 진단, 치료기술, 치료법, 치료제, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 acute SARS-CoV-2 infection amplicons amplification amplified assays binding Cepheid Cepheid Xpert coronavirus Critical detection determine Diagnosis diagnostic diagnostic assay diagnostic tests early diagnosis eight FIVE gene target Genetic genomic gold gold standard highlight Impact knowledge Mutation N gene nucleic acid amplification tests occur pandemic patients PCR product point mutations position Rapid diagnostic test Real-time PCR Region reported respiratory responsible SARS-CoV-2 SARS-CoV-2 genome sequenced Sequencing severe acute respiratory syndrome Coronavirus single-point mutation single-point mutations target the SARS-CoV-2 genome whole genome whole genomes [DOI] 10.1128/Spectrum.01494-21 PMC 바로가기 [Article Type] Research Article
A case report of rhino-facial mucormycosis in a non-diabetic patient with COVID-19: a systematic review of literature and current updateCOVID-19를 가진 비 당뇨병 환자의 코뿔소 점막 점액에 대한 사례 보고서 : 문헌 및 현재 업데이트에 대한 체계적인 검토Case Reports Published on 2021-09-032022-09-01 Journal: BMC Infectious Diseases [Category] SARS, 진단, [키워드] Rhizopus oryzae addition Bacterial bacterial and fungal infections Case report characteristics of patient Corticosteroids COVID-19 COVID-19 cases COVID-19 disease COVID-19 infection debridement diabetes Diabetes Mellitus diagnosis of COVID-19 died discharge during treatment facial Fungal infection fungal infections highlight hospital hyphae Infection invasive lung involvement Lungs male Methylprednisolone Mucormycosis nasal nasal obstruction pathology Patient patient with COVID-19 patients with COVID-19 PCR product physician positive RT-PCR predisposing factor Rhizopus septa sequenced side specimen surgical surgical treatment systematic review the patient treated Treatment was performed [DOI] 10.1186/s12879-021-06625-3 PMC 바로가기 [Article Type] Case Reports
Hairpin-Spherical Nucleic Acids for Diagnosing COVID-19: a Simple Method to Generalize the Conventional PCR for Molecular AssaysCOVID-19 진단을 위한 머리핀-구형 핵산: 분자 분석을 위한 기존 PCR을 일반화하는 간단한 방법Article Published on 2021-07-062022-09-11 Journal: Analytical Chemistry [Category] SARS, 치료기술, 치료제, [키워드] absence acid activator acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Arm AuNP Chain Reaction clinical sample colorimetric detection conserved Conventional coronavirus COVID-19 COVID-19 pandemic detect develop diagnostic diagnostic testing Disease diagnosis DNA DNA polymerase E gene effort exonuclease Final gold Gold nanoparticle Infection Infectious disease Ligand loop modify nucleic acid nucleic acids pandemic Patient PCR PCR cycle PCR product polymerase chain polymerase chain reaction positive presence or absence probe Real-time reverse transcription PCR real-time RT-PCR Region Research reverse transcription PCR RNA RT-PCR SARS-CoV-2 SARS-CoV-2 RNA sensitivity sequence severe acute respiratory syndrome Coronavirus simple STEM TaqMan TaqMan probe [DOI] 10.1021/acs.analchem.1c01515 PMC 바로가기 [Article Type] Article
First Cases of SARS-CoV-2 in Iran, 2020: Case Series ReportCase Series Report Published on 2020-08-012022-10-29 Journal: Iranian Journal of Public Health [Category] COVID-19, [키워드] 2019 novel coronavirus affected China collected conducted Conventional country COVID-19 emerging disease epidemiological feature Future identify increasingly International Iran outbreak PCR product public health emergency Qom report Research reverse transcription PCR RNA RNA polymerase RT-PCR Sanger sequencing SARS-CoV-2 SARS-COV-2 infection sequenced Sery Seven Spread tested Throat swab specimen Transmission virus WHO Wuhan [DOI] 10.18502/ijph.v49i8.3903 PMC 바로가기 [Article Type] Case Series Report
SARS-CoV-2 Titers in Wastewater Are Higher than Expected from Clinically Confirmed CasesResearch Article Published on 2020-07-212022-10-29 Journal: mSystems [Category] COVID-19, [키워드] approach Asymptomatic Clinical course clinically collected complementary Confirmed confirmed case COVID-19 prevalence develop effort epidemiological expected Fatality rate greater help IMPROVE infect Infectious disease Laboratory magnitude Massachusett N gene novel coronavirus SARS-CoV-2 outbreak Outbreaks pandemic PCR product PMMoV positive Precision Prevalence progression of disease protocol qPCR analysis quarantine RT-qPCR S gene Sanger sequencing SARS-CoV-2 SARS-CoV-2 RNA Sequencing analysis significantly significantly higher social distancing Stool Surveillance tested the SARS-CoV-2 titer Treatment viral titer viral titers wastewater [DOI] 10.1128/mSystems.00614-20 PMC 바로가기 [Article Type] Research Article
Molecular epidemiological study of feline coronavirus strains in Japan using RT-PCR targeting nsp14 geneMethodology Article Published on 2015-03-112022-10-28 Journal: BMC veterinary research [Category] Coronavirus, [키워드] addition Amino acid Analysis Blood caused clinically clone coding region conserved contribute coronavirus coronavirus strain detect develop Direct Epidemiological study Epidemiology fatal disease Feline coronavirus Feline infectious peritonitis genetic diversity Genome Genotype genotyping heterogeneous Infection Japan NCBI nsp14 PCR PCR method PCR product performed Phylogenetic analysis phylogenetic study phylogenetically predominant prevalent quasispecy Registered reported Result RT-PCR sequence Sequencing serotypes specimen strain Strains supplementary material TGEV type I variant variants virus detection WGS whole genome sequence [DOI] 10.1186/s12917-015-0372-2 PMC 바로가기 [Article Type] Methodology Article
Human Coronavirus-NL63 infections in Korean children, 2004–2006Article Published on 2006-11-292022-11-01 Journal: Journal of Clinical Virology [Category] Coronavirus, MERS, [키워드] HCoV-NL63 Acute bronchiolitis acute respiratory disease Adenovirus children clinical feature collected Croup described detect excluded found HCoV-NL63 Hospitalized Human IFA Infection influenza A influenza B Korean children MOST MPV nasopharyngeal nasopharyngeal specimen objective parainfluenza PCR assay PCR product positive Prevalence primer reported respiratory tract infection respiratory viruses Result RSV RT-PCR sequenced tested the patient viruses were used [DOI] 10.1016/j.jcv.2006.10.009 PMC 바로가기 [Article Type] Article