Designing and developing a sensitive and specific SARS-CoV-2 RBD IgG detection kit for identifying positive human samplesArticle Published on 2023-03-052024-09-05 Journal: Clinica chimica acta; international journal of cli [Category] update2024, [키워드] bioengineering ELISA Pichia pastoris Receptor-binding domain SARS-CoV-2 Scale-up fermentation [DOI] 10.1016/j.cca.2023.117279 PMC 바로가기 [Article Type] Article
Molecular engineering of a cryptic epitope in Spike RBD improves manufacturability and neutralizing breadth against SARS-CoV-2 variantsArticle Published on 2023-01-272023-07-11 Journal: Vaccine [Category] COVID19(2023년), [키워드] Comirnaty Komagataella phaffii Pichia pastoris protein engineering SARS-CoV-2 RBD Vaccine manufacturing variants of concern [DOI] 10.1016/j.vaccine.2022.12.062 PMC 바로가기
Production and Characterisation of Stabilised PV-3 Virus-like Particles Using Pichia pastoris피치아 파스토리스를 이용한 안정화된 PV-3 바이러스 유사 입자의 생산 및 특성화Article Published on 2022-09-302024-08-26 Journal: Viruses [Category] 폴리오, [키워드] Pichia pastoris Poliovirus Vaccine Virus-like particle
Mucosal nanobody IgA as inhalable and affordable prophylactic and therapeutic treatment against SARS-CoV-2 and emerging variantsArticle Published on 2022-09-122022-11-15 Journal: Frontiers in Immunology [Category] SARS, 변종, 진단, [키워드] administration antibodies Antibody therapeutics Antiviral antiviral prophylaxis and therapeutics Biological sciences comparable conserved effective epitope fusion protein IgA IgG Fc Immunity infected with SARS-CoV-2 Infection intranasal invasive K18-ACE2 less Lineage mammalian Microbiology mucosal nanobody neutralization neutralize omicron Pichia pastoris produced Prophylactic Prophylaxis provide RBD recognize SARS-CoV-2 SARS-CoV-2 spike protein SARS-CoV-2 variant severe respiratory disease Therapeutic treatment transgenic mice variant variants VoC [DOI] 10.3389/fimmu.2022.995412 PMC 바로가기
Steric accessibility of the N-terminus improves the titer and quality of recombinant proteins secreted from Komagataella phaffiiArticle Published on 2022-09-052022-11-16 Journal: Microbial Cell Factories [Category] SARS, 변종, [키워드] addition addressed aggregation Alpha Amino acid applied benefit caused Cell cleavage cleave demonstrated eliminated extension extracellular space functional Golgi highest Host IMPROVE Modification N-terminal N-terminus observation occur peptide Pichia Pichia pastoris product Product quality protease Protein protein engineering protein sequence Proteins purification Receptor binding domain Recombinant protein recombinant proteins residue Saccharomyces cerevisiae secrete secreted secreted protein secretion signal signal peptide steric steric hindrance subunit the SARS-CoV-2 the signal peptide therapeutic vaccine antigen variant variants [DOI] 10.1186/s12934-022-01905-2 PMC 바로가기
COVID-19 management landscape: A need for an affordable platform to manufacture safe and efficacious biotherapeutics and prophylactics for the developing countriesCOVID-19 관리 환경: 개발도상국을 위한 안전하고 효과적인 바이오 치료제 및 예방제를 제조하기 위한 저렴한 플랫폼의 필요성Review Published on 2022-08-262022-09-11 Journal: Vaccine [Category] COVID19(2023년), MERS, SARS, 변종, 신약개발, 진단, [키워드] adopted adoption African antibody approach approaches Asian Biotherapeutics concern country COVID-19 COVID-19 pandemic dependency effective Factors globe help lead less logistics management monoclonal antibodies monoclonal antibody Monoclonal antibody cocktail Multiantigen vaccine National Pichia Pichia pastoris platform prophylactic strategy Prophylactics raw material recombinant vaccine Research Safe SARS-CoV-2 SARS-CoV-2 variants supply the SARS-CoV-2 therapeutic vaccination Vaccine variants variants of concern yeast [DOI] 10.1016/j.vaccine.2022.05.065 PMC 바로가기 [Article Type] Review
Kappa-RBD produced by glycoengineered Pichia pastoris elicited high neutralizing antibody titers against pseudoviruses of SARS-CoV-2 variantsglycoengineered Pichia pasteris에 의해 생산된 Kappa-RBD는 SARS-CoV-2 변이체의 슈도바이러스에 대한 높은 중화 항체 역가를 유발했습니다.Article Published on 2022-04-012022-09-11 Journal: Virology [Category] COVID19(2023년), MERS, SARS, 변종, 진단, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 alum aluminum aluminum hydroxide Antibody titers antigen-specific antibody B.1.617.1 BALB/c mice Beta candidate vaccine coronavirus COVID-19 vaccine CpG domain elicited expression hydroxide India induce Neutralizing antibodies neutralizing antibody Neutralizing antibody titer neutralizing antibody titers Omicron variant Omicron variants Pichia pastoris platform produced pseudoviruses pseudoviruses of SARS-CoV-2 RBD Receptor-binding domain Receptor-binding domain (RBD) respiratory SARS-CoV-2 SARS-CoV-2 kappa severe acute respiratory syndrome Coronavirus the epidemic Vaccine variant VoC was used wild-type yeast [DOI] 10.1016/j.virol.2022.03.001 PMC 바로가기 [Article Type] Article
Covalent coupling of Spike’s receptor binding domain to a multimeric carrier produces a high immune response against SARS-CoV-2스파이크의 수용체 결합 도메인의 다층 담체에 대한 공유 커플 링은 SARS-COV-2에 대해 높은 면역 반응을 생성한다.Article Published on 2022-01-132022-09-01 Journal: Scientific Reports [Category] 진단, [키워드] Antigen presentation average Biochemistry Brucella candidate Candidates composed COVID-19 vaccine develop dose effective elicited expressed HEK-293T cells humoral immune response IgGs immune response immune system immunogenic immunology induce mammalian mice Neutralizing Neutralizing antibodies Particle Pichia pastoris produced Protein RBD Receptor binding domain reported SARS-CoV-2 significantly higher Spike protein Synthase the Spike vaccinate Vaccine were used [DOI] 10.1038/s41598-021-03675-0 PMC 바로가기 [Article Type] Article
Glycosylation of Receptor Binding Domain of SARS-CoV-2 S-Protein Influences on Binding to Immobilized DNA AptamersArticle Published on 2022-01-052022-10-29 Journal: International Journal of Molecular Sciences [Category] COVID-19, [키워드] affected amino acid substitution aptamer Aptamers binding Biolayer interferometry cell line Chinese hamster coronavirus development dissociation constants DNA Effects Efficacy element expressed expression glycosylation glycosylation patterns influence inhibitor new virus Pichia pastoris RBD Receptor binding domain Receptor-binding domain recognizing S-protein SARS-CoV-2 sensor surface spike glycoprotein tested the RBD the RBD protein therapy variant viral infection viral strain virion [DOI] 10.3390/ijms23010557 PMC 바로가기 [Article Type] Article
Production of a Hepatitis E Vaccine Candidate Using the Pichia pastoris Expression SystemArticle Published on 2022-01-012023-06-11 Journal: Methods in molecular biology (Clifton, N.J.) [Category] E형 간염, [키워드] hepatitis E virus open reading frame 2 Pichia pastoris Virus-like particle [DOI] 10.1007/978-1-0716-1892-9_7