Effect of wash media type during PBMC isolation on downstream characterization of SARS-CoV-2-specific T cellsArticle Published on 2023-06-292024-09-05 Journal: Journal of Immunological Methods [Category] update2024, [키워드] Centrifugation brakes flow cytometry FluoroSpot processing T cell Wash media [DOI] 10.1016/j.jim.2023.113520 PMC 바로가기 [Article Type] Article
Performance of nasopharyngeal swab and saliva in detecting Delta and Omicron SARS-CoV-2 variantsDelta 및 Omicron SARS-CoV-2 변이체 검출 시 비인두 면봉 및 타액의 성능Article Published on 2022-10-012022-09-11 Journal: Journal of medical virology [Category] COVID19(2023년), MERS, SARS, 변종, 진단, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus analyzed Chain Reaction characterized conducted coronavirus cough COVID-19 Delta diagnostic Dyspnea epidemic wave epidemic waves expressed highest increase in Infection Laboratory log molecular nasopharyngeal Nasopharyngeal swab no significant difference not significant NP swab NP swabs NPs nucleic acids omicron participant Participants Patient performance polymerase chain polymerase chain reaction processing prospective cohort study real-time RT-PCR reported required respiratory rhinorrhea RNA Saliva saliva samples Sample SARS-CoV-2 SARS-CoV-2 diagnostics SARS-CoV-2 epidemic SARS-CoV-2 variant significant difference sore sore throat specimen specimen collection statistically Symptom Symptoms thawing the mean transported Viral Viral load [DOI] 10.1002/jmv.27898 PMC 바로가기 [Article Type] Article
A Natural Language Processing (NLP) Evaluation on COVID-19 Rumour Dataset Using Deep Learning TechniquesArticle Published on 2022-09-142022-11-16 Journal: Computational Intelligence and Neuroscience [Category] 치료기술, [키워드] Accuracy adopted affected Algorithm Algorithms Alter Anxiety applied approaches collected Community Confusion Context coronavirus COVID-19 dataset datasets deep deep learning Depression detect Effectiveness Epidemic evaluated experiment filtered Google information language learning natural network objective outbreak parameter Precision processing promote recall Research Result resulting significantly simple technique Temporal the disease virus website [DOI] 10.1155/2022/6561622 PMC 바로가기
Human coronaviruses disassemble processing bodiesArticle Published on 2022-08-232022-11-16 Journal: PLoS Pathogens [Category] SARS, 유전자 메커니즘, [키워드] 229E caused cells characteristic common cold CoVs contribute coronavirus Coronavirus infection correlated CoVs cytokine cytoplasmic dysregulated dysregulation expression FIVE fluorescent Granule Human human coronavirus IL-6 immune response impacted infected cell Infection information innate immune response mechanism MERS mRNA transcripts NL63 OC43 processing Proinflammatory cytokine proinflammatory cytokines promote Protein respond responsible ribonucleoprotein RNA RNAs SARS-CoV SARS-CoV-2 SARS-CoV-2 N SARS-CoV-2 N protein screened severe SARS-CoV-2 stability submission the N protein the viral nucleocapsid TNF transcript translation unique viral infection virus [DOI] 10.1371/journal.ppat.1010724 PMC 바로가기
Flipped over U: structural basis for dsRNA cleavage by the SARS-CoV-2 endoribonuclease뒤집힌 U: SARS-CoV-2 엔도리보뉴클레아제에 의한 dsRNA 절단의 구조적 기초Article Published on 2022-08-122022-09-11 Journal: Nucleic Acids Research [Category] SARS, 유전자 메커니즘, 치료법, [키워드] activate Activation active site cleavage cleave coronavirus Coronavirus replication Critical cryo-EM structure cryo-EM structures double-stranded dsRNA endoribonuclease evade form generate Host immune MDA5 mechanism Nsp15 Pattern recognition pattern recognition receptor platform processing recognize replication-transcription complex RNA SARS-CoV-2 SARS-CoV-2 Nsp15 single-stranded site-directed mutagenesis structures substrates the SARS-CoV-2 uridine Vesicle viral replication [DOI] 10.1093/nar/gkac589 PMC 바로가기 [Article Type] Article
Comparison of two T-cell assays to evaluate T-cell responses to SARS-CoV-2 following vaccination in naïve and convalescent healthcare workers미경험 및 회복기 의료 종사자를 대상으로 백신 접종 후 SARS-CoV-2에 대한 T 세포 반응을 평가하기 위한 두 가지 T 세포 분석 비교Comparative Study Published on 2022-07-222022-09-11 Journal: Clinical and experimental immunology [Category] COVID19(2023년), MERS, SARS, 진단, 치료기술, 치료법, [키워드] acquisition antibody Antibody responses AZD1222 Blood BNT162b ChAdOx1 ChAdOx1 nCoV-19 characterized complex convalescent correlated strongly correlation dose ELISPOT ELISpot assay evaluate HCWs healthcare worker implication Infection less magnitude measure membrane moderate naïve Nucleocapsid proteins Oxford Panel pathway patient population peptides Pfizer Pfizer/BioNTech processing proportion protocol Region reported Reporting representing Research response responses SARS-CoV-2 SARS-CoV-2 spike SARS-CoV-2 spike protein seasonal coronaviruses significantly lower Spike protein Spike-specific T-cell responses Standardization T cell T-cell T-cell assay T-cell Response T-cell responses vaccination Vaccine vaccinees virus [DOI] 10.1093/cei/uxac042 PMC 바로가기 [Article Type] Comparative Study
Coordination of SARS-CoV-2 wastewater and clinical testing of university students demonstrates the importance of sampling duration and collection timeSARS-CoV-2 폐수 및 대학생의 임상 시험의 조정은 샘플링 기간 및 수집 시간의 중요성을 보여줍니다Article Published on 2022-07-152022-09-11 Journal: The Science of the total environment [Category] COVID19(2023년), SARS, 치료기술, [키워드] Analysis approach Clinical data clinical testing collected Collection complexes Concentration control sample controls coordination correlated COVID-19 COVID-19 pandemic epidemiological Epidemiology evaluated fecal infected individuals Local Method development methodology not different occur populations positive preparation processing provide sample collection SARS-CoV-2 SARS-CoV-2 detection sensitivity shedding shown significantly higher spring Surveillance test result trajectory university virus detection Virus recovery wastewater Wastewater-based epidemiology [DOI] 10.1016/j.scitotenv.2022.154619 PMC 바로가기 [Article Type] Article
Production of a functionally active recombinant SARS-CoV-2 (COVID-19) 3C-like protease and a soluble inactive 3C-like protease-RBD chimeric in a prokaryotic expression system원핵생물 발현 시스템에서 기능적으로 활성인 재조합 SARS-CoV-2(COVID-19) 3C 유사 프로테아제 및 가용성 불활성 3C 유사 프로테아제-RBD 키메라의 생산Article Published on 2022-06-202022-09-11 Journal: Epidemiology and Infection [Category] COVID19(2023년), SARS, 치료제, [키워드] 3C-like protease acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addition antigenicity approach blocked carrier cells Chimer chimeric chymotrypsin co-expressed complex coronavirus COVID-19 COVID-19 vaccines cysteine cysteine protease cysteine proteases dimeric disease drug ELISAs enzyme Escherichia coli Escherichia coli BL21 exhibit expression function inactive inhibitors inhibitory main protease non-structural protein non-structural proteins Papain Papain-like protease PLPro polyproteins pp1a pp1ab processing produced Production prokaryotic expression system protease Proteases Protein purification RBD Receptor binding domain RTC SARS-CoV-2 Serine serum severe acute respiratory syndrome Coronavirus solubility Spike protein target tested the Spike Transcription vaccinated individual vaccinated individuals viral replication [DOI] 10.1017/S0950268822001078 PMC 바로가기 [Article Type] Article
Detection of SARS-CoV-2 RNA throughout wastewater treatment plants and a modeling approach to understand COVID-19 infection dynamics in Winnipeg, Canada폐수 처리장 전체에서 SARS-CoV-2 RNA 검출 및 캐나다 위니펙의 COVID-19 감염 역학을 이해하기 위한 모델링 접근 방식Article Published on 2022-06-152022-09-11 Journal: The Science of the total environment [Category] COVID19(2023년), SARS, 치료기술, 치료법, [키워드] applied approach Back-trajectory modeling Canada Concentration Consensus correlation correlations COVID-19 infection Data analysis detection discharge Final fluctuation fluctuations fraction indicated infected individual Infection initial plant plants positive control processing reached reduction RNA RNA concentrations sample collection SARS-CoV-2 SARS-CoV-2 RNA sensitivity Sludge suggested supernatant Surveillance Treatment virus virus concentration wastewater Wastewater solids wastewater surveillance water water cycle Winnipeg [DOI] 10.1016/j.scitotenv.2022.153906 PMC 바로가기 [Article Type] Article
Functional properties of the spike glycoprotein of the emerging SARS-CoV-2 variant B.1.1.529신흥 SARS-CoV-2 변이체 B.1.1.529의 스파이크 당단백질의 기능적 특성Article Published on 2022-06-142022-09-11 Journal: Cell Reports [Category] COVID19(2023년), SARS, 변종, [키워드] ACE2 ACE2 affinity ACE2 binding acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 B.1.1.529 B.1.617.2 Cell coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 CP: Immunology CP: Microbiology cytopathic effects D614G decrease exhibit functional glycoprotein higher affinity immune evasion immunology Infection less Microbiology neutralization neutralization sensitivity omicron Patient prevalent processing property pseudovirus pseudoviruses relative S glycoprotein S glycoproteins S trimer S1 shedding SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2 variants sera severe acute respiratory syndrome Coronavirus spike glycoprotein stability syncytium formation temperature TMPRSS2 TMPRSS2 expression Transmission unliganded vaccinees variant variants virion [DOI] 10.1016/j.celrep.2022.110924 PMC 바로가기 [Article Type] Article