Small ubiquitin-like modifier-tag and modified protein purification significantly increase the quality and quantity of recombinant African swine fever virus p30 proteinResearch Article Published on 2024-05-222024-09-05 Journal: Veterinary World [Category] update2024, [키워드] African swine fever Diagnosis indirect enzyme-linked immunosorbent assay p30 protein expression purification [DOI] 10.14202/vetworld.2024.1157-1167 PMC 바로가기 [Article Type] Research Article
Protein expression/secretion boost by a novel unique 21-mer cis -regulatory motif (Exin21) via mRNA stabilizationOriginal Article Published on 2023-04-052024-09-05 Journal: Molecular therapy : the journal of the American So [Category] update2024, [키워드] antibody cis-regulation mRNA oligonucleotide motif protein expression protein secretion SARS-CoV-2 Vaccine [DOI] 10.1016/j.ymthe.2023.02.012 PMC 바로가기 [Article Type] Original Article
Jinhua Qinggan granules attenuates acute lung injury by promotion of neutrophil apoptosis and inhibition of TLR4/MyD88/NF-κB pathwayArticle Published on 2023-01-302022-11-16 Journal: Journal of ethnopharmacology [Category] 신종인플루엔자, 치료기술, [키워드] acute lung injury analyzed Anti-inflammation Apoptosis approved attenuate BALF Bcl-xL caspase Cell changes China confocal microscope coronavirus COVID-19 declined downstream drug Effect expression fatal complication flow cytometry food Granule H1N1 influenza histological histopathological IL-1β IL-6 immunofluorescence Infectious disease Influenza virus inhibited injection intrinsic investigated Jinhua Qinggan granules LPS lung Lung injury Lungs material mechanism mice Mild mitochondrial apoptotic pathway moderate morbidity and mortality MPO neutrophil Neutrophil apoptosis novel coronavirus disease Nuclear factor kappa-B nuclei p65 pathway pharmacological positive proportion Protein protein concentration protein expression pulmonary inflammation receptor reduced Respiratory disease Respiratory virus infection Signaling signaling pathway significantly staining survival survival rate TLR4 TNF-α toll-like receptor 4 treated Treatment were assessed were measured [DOI] 10.1016/j.jep.2022.115763 PMC 바로가기
Spike protein receptor-binding domains from SARS-CoV-2 variants of interest bind human ACE2 more tightly than the prototype spike proteinArticle Published on 2023-01-222023-07-11 Journal: Biochemical and Biophysical Research Communication [Category] COVID19(2023년), [키워드] analytical ultracentrifugation COVID-19 Isothermal calorimetry Protein binding protein expression Receptor binding domain Spike protein [DOI] 10.1016/j.bbrc.2022.12.011 PMC 바로가기
Expression of Large Full-Length PfEMP1 Proteins in HEK293 Cellsarticle Published on 2022-12-312024-09-01 Journal: Methods in molecular biology (Clifton, N.J.) [Category] 말라리아, [키워드] Full-length VAR2CSA HEK293 malaria PfEMP1 Plasmodium falciparum protein expression [Article Type] article
Accessing isotopically labeled proteins containing genetically encoded phosphoserine for NMR with optimized expression conditionsArticle Published on 2022-12-012023-07-10 Journal: The Journal of Biological Chemistry [Category] COVID19(2023년), [키워드] genetic code expansion nuclear magnetic resonance phosphoserine post-translation modification protein expression protein phosphorylation protein synthesis. [DOI] 10.1016/j.jbc.2022.102613 PMC 바로가기
Induction of pulmonary HLA-G expression by SARS-CoV-2 infectionArticle Published on 2022-11-052022-11-15 Journal: Cellular and Molecular Life Sciences [Category] SARS, 치료기술, 치료제, [키워드] accompanied Antigen Autopsy biopsies CD8 Cell changes in correlated distribution epithelial cell Evidence expressed expression expression level Frequency heart muscle HLA-G Hyperactivation Immune cell Immune cell infiltration immune cells Immune escape immune response induction information kidney leukocyte lung lung alveolar lung epithelia lung epithelial cell lung tissue Macrophage marker microRNA miRNA miRNA expression modulating organs other organ patients protein expression SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 infections SARS-CoV-2 nucleocapsid SARS-CoV-2- SARS-CoV-2-infected patient specimen Spike protein surgical T cell responses T cells therapeutic target tissue tissues Treatment Tumor virus-infected cells while [DOI] 10.1007/s00018-022-04592-9 PMC 바로가기
Proteomic analysis and identification reveal the anti-inflammatory mechanism of clofazimine on lipopolysaccharide-induced acute lung injury in miceArticle Published on 2022-11-012022-11-15 Journal: Inflammation research : official journal of the Eu [Category] 유전자 메커니즘, 진단, [키워드] acute lung injury acute respiratory distress acute respiratory distress syndrome alleviate Analysis Anti-inflammatory anti-inflammatory property anti-inflammatory response ARDS CFZ Clofazimine coronavirus disease COVID-19 Critical decrease disease Diseases distress down-regulate histological change hyperimmune response IL-1β immune IMPROVE increasingly Infectious disease Inflammatory information inhibit involved lipopolysaccharide LPS lung Lung injury Macrophage macrophages mechanism mice Molecular biology pathway pharmacological mechanism potential molecular profile proliferation protective effect Protein protein expression Proteomic analysis proteomics Pulmonary edema Quantitative Signal transduction Signaling significantly survival rate syndrome therapeutic strategy TNF-α [DOI] 10.1007/s00011-022-01623-w PMC 바로가기
Optimization of the heterologous expression and purification of Plasmodium falciparum generative cell specific 1 in Escherichia coliarticle Published on 2022-10-312024-09-01 Journal: Protein Expression and Purification [Category] 말라리아, [키워드] Malaria vaccine Plasmodium falciparum generative cell specific 1 (GCS1) protein expression [Article Type] article
CASA: a comprehensive database resource for the COVID-19 Alternative Splicing AtlasArticle Published on 2022-10-202022-11-15 Journal: Journal of Translational Medicine [Category] 유전자 메커니즘, 치료기술, 치료제, [키워드] affected alteration Analysis Atla cell line conditions COVID-19 database dataset Diseases drug-target interaction event events facilitate functional annotation help host cell human cell identify immune response IMPROVE Infectious disease mechanism mechanisms Organoid performed platform profile Protein protein expression provided Quantitative data regulate Regulatory Research resource RNA RNA-seq dataset severity splicing tissues transcriptional virus [DOI] 10.1186/s12967-022-03699-8 PMC 바로가기