Production of Reverse Transcriptase and DNA Polymerase in Bacterial Expression SystemsArticle Published on 2024-07-182024-09-05 Journal: Bioengineering [Category] update2024, [키워드] DNA polymerase Escherichia coli extracellular production protein production reverse transcriptase Vibrio natriegens [DOI] 10.3390/bioengineering11070727 PMC 바로가기 [Article Type] Article
Highly efficient protein expression of Plasmodium vivax surface antigen, Pvs25, by silkworm and its biochemical analysisarticle Published on 2022-08-312024-09-01 Journal: Protein Expression and Purification [Category] 말라리아, [키워드] malaria protein production Pvs25 Silkworm Vaccine [Article Type] article
Expression, purification and characterization of SARS-CoV-2 spike RBD in ExpiCHO cellsExpiCHO 세포에서 SARS-CoV-2 스파이크 RBD의 발현, 정제 및 특성화Article Published on 2022-06-012022-09-11 Journal: Protein expression and purification [Category] COVID19(2023년), MERS, SARS, 변종, 진단, [키워드] antibody Antibody binding assay Antigen binding binding domain Blotting collection time Critical Culture culture medium Diagnosis diagnostic effective element ELISA test eukaryotic evaluate evaluated ExpiCHO cell ExpiCHO cells expressed Final flow cytometer functional good quality identify immune response Infection Interferometry Isolation mass mass spectrometry medium monomeric mutants Protein protein production protocol purification purified RBD Receptor binding domain receptor binding domain (RBD) robust SARS-CoV-2 SARS-CoV-2 spike SARS-CoV-2 Spike RBD SARS-CoV-2 Spike. SDS-PAGE selective Spectrometry spike the RBD transiently transfected Vaccines virus western blotting [DOI] 10.1016/j.pep.2022.106071 PMC 바로가기 [Article Type] Article
The Nuts and Bolts of SARS-CoV-2 Spike Receptor-Binding Domain Heterologous ExpressionSARS-COV-2 스파이크 수용체 결합 도메인 이종 발현의 견과류 및 볼트Article Published on 2021-12-022022-09-01 Journal: Biomolecules [Category] SARS, 유전자 메커니즘, 진단, [키워드] addition caused Circular coronavirus COVID-19 denaturation diagnostic domain effective ELISA Escherichia coli evaluated experiment filtration flow cytometry assay flow cytometry assays gel gel filtration HEK-293 cells heterologous expression indicated Infectious disease Neutralizing antibodies neutralizing antibody Nut Outbreaks pandemic produced progressed Protein protein production RBD Receptor-binding domain Recombinant SARS-CoV-2 RBD SARS-CoV-2 spike Spike protein surface Spike the SARS-CoV-2 Vaccine [DOI] 10.3390/biom11121812 PMC 바로가기 [Article Type] Article
Improved production of SARS-CoV-2 spike receptor-binding domain (RBD) for serology assays혈청학 분석을 위한 SARS-CoV-2 스파이크 수용체 결합 도메인(RBD) 생산 개선Article Published on 2021-03-012022-09-12 Journal: Protein expression and purification [Category] SARS, 진단, [키워드] Antigen assays can be used Cell culture COVID-19 Critical demonstrated DNA domain element ELISA expression form functional IMPROVE Improved investigated parameters peptides promoter Protein protein expression protein production purification RBD reagent Receptor-binding domain responsible robust SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 spike sensitivity serology serology assay serology assays Spike protein Support the RBD protein the SARS-CoV-2 variable [DOI] 10.1016/j.pep.2020.105802 PMC 바로가기 [Article Type] Article
Optimizing high-yield production of SARS-CoV-2 soluble spike trimers for serology assaysArticle Published on 2020-10-012023-07-11 Journal: Protein expression and purification [Category] SARS, [키워드] COVID-19 ELISA protein production SARS-CoV-2 serology Spike protein [DOI] 10.1016/j.pep.2020.105686 PMC 바로가기