Colon-derived Caco-2 cells support replication of hepatitis E virus genotype 1 strain Sar55 generated by reverse geneticsArticle Published on 2024-06-272024-09-05 Journal: Virus Research [Category] update2024, [키워드] Cell culture genotype 1 hepatitis E virus intestine reverse genetics Sar55 [DOI] 10.1016/j.virusres.2024.199427 PMC 바로가기 [Article Type] Article
The Full-Genome Analysis and Generation of an Infectious cDNA Clone of a Genotype 6 Hepatitis E Virus Variant Obtained from a Japanese Wild Boar: In Vitro Cultivation in Human Cell LinesArticle Published on 2024-05-242024-09-05 Journal: Viruses [Category] update2024, [키워드] Cell culture hepatitis E virus HEV-6 variant Infectious cDNA clone reverse genetics Wild boar [DOI] 10.3390/v16060842 PMC 바로가기 [Article Type] Article
A novel BSL-2 Lassa virus reverse genetics system modelling the complete viral life cycleResearch Article Published on 2024-05-152024-09-05 Journal: Emerging Microbes & Infections [Category] update2024, [키워드] Antiviral Lassa virus minigenome reverse genetics ribavirin [DOI] 10.1080/22221751.2024.2356149 PMC 바로가기 [Article Type] Research Article
A rapid and versatile reverse genetics approach for generating recombinant positive-strand RNA viruses that use IRES-mediated translationVirus-Cell Interactions Published on 2024-03-142024-09-05 Journal: Journal of virology [Category] update2024, [키워드] cap-independence IRES reporter virus reverse genetics RNA virus [DOI] 10.1128/jvi.01638-23 PMC 바로가기 [Article Type] Virus-Cell Interactions
Construction and characterization of an infectious cDNA clone of human rhinovirus A89Research Article Published on 2024-02-282024-09-05 Journal: Heliyon [Category] update2024, [키워드] Infectious cDNA clone reverse genetics Rhinovirus A89 virulence [DOI] 10.1016/j.heliyon.2024.e27214 PMC 바로가기 [Article Type] Research Article
Characterization of intrinsic and effective fitness changes caused by temporarily fixed mutations in the SARS-CoV-2 spike E484 epitope and identification of an epistatic precondition for the evolution of E484A in variant Omicronarticle Published on 2023-11-082024-09-05 Journal: Virology Journal [Category] update2024, [키워드] Antibody neutralization escape COVID-19 Epitope E484 reverse genetics SARS-CoV-2 Spike protein evolution virus fitness [DOI] 10.1186/s12985-023-02154-4 PMC 바로가기 [Article Type] article
Establishment of a CPER reverse genetics system for Powassan virus defines attenuating NS1 glycosylation sites and an infectious NS1-GFP11 reporter virusarticle Published on 2023-07-252024-09-05 Journal: mBio [Category] update2024, [키워드] attenuation cell-to-cell spread circular polymerase extension reaction flavivirus NS1 glycosylation mutants Powassan virus reverse genetics split-GFP [DOI] 10.1128/mbio.01388-23 PMC 바로가기 [Article Type] article
An in vitro workflow to create and modify infectious clones using replication cycle reactionArticle Published on 2023-06-212024-09-05 Journal: Virology [Category] update2024, [키워드] dengue virus infectious clones Mutagenesis reverse genetics SARS-CoV-2 [DOI] 10.1016/j.virol.2023.05.013 PMC 바로가기 [Article Type] Article
In SARS-CoV-2 delta variants, Spike-P681R and D950N promote membrane fusion, Spike-P681R enhances spike cleavage, but neither substitution affects pathogenicity in hamstersArticle Published on 2023-04-102024-09-05 Journal: eBioMedicine [Category] update2024, [키워드] COVID-19 hamster reverse genetics SARS-CoV-2 [DOI] 10.1016/j.ebiom.2023.104561 PMC 바로가기 [Article Type] Article
A Rapid Method for Generating Infectious SARS-CoV-2 and Variants Using Mutagenesis and Circular Polymerase Extension Cloningarticle Published on 2023-03-062024-09-05 Journal: Microbiology Spectrum [Category] update2024, [키워드] CPEC infectious clone Mutagenesis reverse genetics SARS-CoV-2 [DOI] 10.1128/spectrum.03385-22 PMC 바로가기 [Article Type] article