Ultrasensitive and amplification-free detection of SARS-CoV-2 RNA using an electrochemical biosensor powered by CRISPR/Cas13aArticle Published on 2023-01-042024-09-05 Journal: Bioelectrochemistry (Amsterdam, Netherlands) [Category] update2024, [키워드] CRISPR electrochemical biosensor ORF1ab gene S gene SARS-CoV-2 RNA [DOI] 10.1016/j.bioelechem.2023.108364 PMC 바로가기 [Article Type] Article
Molecular evolutionary characteristics of OBI virus S gene among the adolescent population in rural and pastoral areas of Xinjiang ProvinceArticle Published on 2023-01-012023-06-12 Journal: Infection, genetics and evolution : journal of mol [Category] B형 간염, [키워드] Adolescent population molecular evolution Occult hepatitis B virus infection (OBI) S gene [DOI] 10.1016/j.meegid.2022.105395
The spike gene target failure (SGTF) genomic signature is highly accurate for the identification of Alpha and Omicron SARS-CoV-2 variantsArticle Published on 2022-11-082022-11-15 Journal: Scientific Reports [Category] SARS, 변종, 유전자 메커니즘, 치료기술, 치료제, [키워드] 95% CI Accuracy Alpha variant Amino acid Analysis B.1.1.529 B.1.1.7 cause Del detect evaluated genomic Laboratory Mutation omicron positive Positive predictive value Protein resulting RT-PCR S gene SARS-CoV-2 SARS-CoV-2 RT-PCR SARS-CoV-2 variant Sensitivity and specificity sequenced Sequencing spike gene TaqPath tested the Spike Thermo Fisher variant variants of concern VoC [DOI] 10.1038/s41598-022-21564-y PMC 바로가기
The mutation features and geographical distributions of the surface glycoprotein (S gene) in SARS-CoV-2 strains: A comparative analysis of the early and current strainsArticle Published on 2022-11-012022-11-16 Journal: Journal of medical virology [Category] SARS, 변종, [키워드] ACE2-binding region acute respiratory syndrome Analysis analyzed coronavirus coronavirus disease COVID-19 COVID-19 vaccines demonstrated develop Evolution feature Frequency Genome genome mutations geographical distribution highest identify loci mutant strain Mutation omicron prevalent remained Result S gene S protein SARS-CoV-2 SARS-CoV-2 strain SARS-CoV-2 variant strain Strains Surface glycoprotein the receptor-binding domain the S protein The United States United Kingdom Vaccines was used [DOI] 10.1002/jmv.28023 PMC 바로가기
Comparison of viral propagation and drug response among SARS-CoV-2 VOCs using replicons capable of recapitulating virion assembly and releaseArticle Published on 2022-10-012022-11-15 Journal: Virologica Sinica [Category] COVID19(2023년), SARS, 변종, 치료제, [키워드] acute respiratory syndrome assays Beta changed coronavirus Delta delta VoCs drug resistance drug response ENhance Evolution of SARS-CoV-2 genomes immune evasion inhibitor manifested Mutation novel antiviral drugs Protein provide quantification released remained Remdesivir replicon RNA S gene SARS-CoV-2 sgRNA supernatant Support Transcription variants variants of concern variants of concern (VOC) viral genome replication viral infectivity viral life cycle viral propagation Viral protein Viral-like particle (VLP). viral-like particles virion assembly VLP VoC VOCs WIV04 strain [DOI] 10.1016/j.virs.2022.06.008 PMC 바로가기
Automated, portable, and high-throughput fluorescence analyzer (APHF-analyzer) and lateral flow strip based on CRISPR/Cas13a for sensitive and visual detection of SARS-CoV-2SARS-CoV-2의 감도 및 시각적 검출을 위한 CRISPR/Cas13a 기반의 자동화된 휴대용 고처리량 형광 분석기(APHF 분석기) 및 측면 흐름 스트립Article Published on 2022-10-012022-09-11 Journal: Talanta [Category] COVID19(2023년), SARS, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addressed amplification automated biosensor Care Contamination coronavirus COVID-19 CRISPR CRISPR/Cas13a detect Detection limit develop globe Health Lateral lateral flow strip MOST On-site detection ORF1ab gene POCT Point point of care Point of care testing (POCT) polymerase provided recombinase polymerase amplification Recombinase polymerase amplification (RPA) RPA S gene SARS-CoV-2 SARS-CoV-2 detection severe acute respiratory syndrome Coronavirus specificity Visualization [DOI] 10.1016/j.talanta.2022.123594 PMC 바로가기 [Article Type] Article
RT-LAMP assay combining multi-fluorescent probes for SARS-CoV-2 RNA detection and variant differentiationSARS-CoV-2 RNA 검출 및 변이체 분화를 위한 다중 형광 프로브를 결합한 RT-LAMP 분석Article Published on 2022-10-012022-09-11 Journal: Talanta [Category] COVID19(2023년), SARS, 변종, 치료기술, 치료제, [키워드] addition applied can be used clinical setting clinical settings detect Fluorescent probes Genes isothermal amplification limit of detection Loop-mediated isothermal amplification molecular mutant Mutation N gene observation ORF1 ORF1ab P681H P681R positive result primer set probe quantification regions reverse transcription Reverse transcription loop-mediated isothermal amplification (RT-LAMP) RT-LAMP S gene SARS-CoV-2 SARS-CoV-2 RNA SARS-CoV-2 RNA detection SARS-CoV-2 viral RNA sensitivity simple specificity Spread Transcription type strain variant Variant differentiation. viral RNA detection virus wild type wild type strain wild-type [DOI] 10.1016/j.talanta.2022.123644 PMC 바로가기 [Article Type] Article
Immediate reinfection with Omicron variant after clearance of a previous SARS-CoV-2 infectionArticle Published on 2022-09-012022-11-16 Journal: Journal of infection and public health [Category] COVID19(2023년), SARS, 변종, [키워드] Alpha variant clearance Combination COVID-19 Delta epidemic peak heterogeneity Immediate immune evasion individuals Infection Italy Lineage Microbiology omicron Patient Primary infection Regional Reinfection RT-PCR test S gene SARS-CoV-2 SARS-COV-2 infection searched Sequencing Transmissibility variant variants whole genome [DOI] 10.1016/j.jiph.2022.07.013 PMC 바로가기
Development of a simple genotyping method based on indel mutations to rapidly screen SARS-CoV-2 circulating variants: Delta, Omicron BA.1 and BA.2SARS-CoV-2 순환 변이체: Delta, Omicron BA.1 및 BA.2를 신속하게 스크리닝하기 위한 indel 돌연변이에 기반한 간단한 유전형 분석법 개발Article Published on 2022-09-012022-09-11 Journal: Journal of virological methods [Category] COVID19(2023년), SARS, 변종, 치료기술, 치료제, [키워드] Analytical specificity circulating circulating SARS-CoV-2 variants concern Concordance COVID-19 case Delta Delta VOC develop development facilitate flexible genotyping identify implementation melting curve analysis Mutation mutations omicron Partial partial sequencing performed primer public health qPCR assay qPCR assays S gene SARS-CoV-2 Screening screening method Sequencing verification VOCs [DOI] 10.1016/j.jviromet.2022.114570 PMC 바로가기 [Article Type] Article
SARS-CoV-2 Brain Regional Detection, Histopathology, Gene Expression, and Immunomodulatory Changes in Decedents with COVID-19코로나19 사망자의 SARS-CoV-2 뇌 부위 검출, 조직병리학, 유전자 발현 및 면역조절 변화Article Published on 2022-08-162022-09-11 Journal: Journal of neuropathology and experimental neurolo [Category] SARS, 유전자 메커니즘, 치료기술, [키워드] Amygdala arising Autopsies Autopsy Blood Brain brains cerebellar cortex change Control COVID-19 COVID-19 cases cytokine Deafferentation detection Differentially expressed genes downregulation Encephalitis fraction Gene Expression Genes Hemorrhagic hemorrhagic encephalitis Histopathology immune immune response indicated involved marker microglia mucosa neuronal Non-COVID-19 olfactory olfactory bulb portal receptor reduced reduction Region Regional RNA polymerase RNA sequence RNA sequences RNA-dependent RNA polymerase RNAseq RT-PCR S gene SARS-CoV-2 SARS-CoV-2 E gene sequence serum Significant subject upregulation viral sequence virus with COVID-19 [DOI] 10.1093/jnen/nlac056 PMC 바로가기 [Article Type] Article