Protease-Responsive Peptide-Conjugated Mitochondrial-Targeting AIEgens for Selective Imaging and Inhibition of SARS-CoV-2-Infected CellsArticle Published on 2022-08-232022-11-15 Journal: ACS Nano [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] acute respiratory syndrome aggregation Cell cleavage coronavirus cytotoxicity effective Efficiency Health imaging induce infected cell infected cells inhibition inhibitor lack M pro main protease mitigate mitochondrial mitochondrial targeting peptide peptide-conjugated AIEgen Plasmid Prevent probe protease recombinant proteins SARS-CoV-2 SARS-CoV-2-infected cell SARS-CoV-2-infected cells selective therapeutic process transfected with Treatment triggering Viral transmission virus virus theranostics. [DOI] 10.1021/acsnano.2c03219 PMC 바로가기
Cell surface SARS-CoV-2 nucleocapsid protein modulates innate and adaptive immunity세포 표면 SARS-CoV-2 뉴클레오캡시드 단백질은 선천성 및 후천성 면역을 조절합니다Article Published on 2022-08-052022-09-11 Journal: Science Advances [Category] SARS, 변종, 치료기술, 치료법, 치료제, [키워드] activate adaptive antibody antigenicity bind Cell cell surface cells chemokine chemokines chemotaxis Combination conserved CoVs cross-reactive cytosol detect electrostatic heparan sulfate heparin high affinity high-affinity binding human CoVs immune cells Immunity induce infected cells inhibited Innate immune cells Innate immunity leukocyte leukocytes live cells MERS-CoV modulate neighboring cell neighboring cells nucleocapsid protein Protein released SARS-CoV-1 SARS-CoV-2 SARS-CoV-2 nucleocapsid SARS-CoV-2 variant SARS-CoV-2 variants SARS-CoV-2-infected cell SARS-CoV-2-infected cells sequestering Strains sulfate sulfated T cell T cell responses transfected cell Vaccine zoonotic [DOI] 10.1126/sciadv.abp9770 PMC 바로가기 [Article Type] Article
Specific intracellular signature of SARS-CoV-2 infection using confocal Raman microscopy Research Published on 2022-07-252022-10-05 Journal: Communications Chemistry [Category] 바이오마커, 진단, [키워드] Accuracy amino-acid analyzed biochemical Biomarkers Cell contribute COVID-19 Data analysis demonstrated Factor identify infected cell Infection Interaction Microscopy Modification molecular non-infected Raman spectroscopy RNA virus RNA viruses SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2-infected cells significant increase Specific Spread target therapeutic intervention viral pathogenesis virus Virus-host interactions virus-infected cell [DOI] 10.1038/s42004-022-00702-7 [Article Type] Research
The Cellular Characterization of SARS-CoV-2 Spike Protein in Virus-Infected Cells Using the Receptor Binding Domain Binding Specific Human Monoclonal Antibodies수용체 결합 도메인 결합 특이적 인간 단일클론 항체를 사용하여 바이러스에 감염된 세포에서 SARS-CoV-2 스파이크 단백질의 세포 특성화Article Published on 2022-07-132022-09-11 Journal: Journal of Virology [Category] COVID19(2023년), MERS, SARS, 변종, 진단, [키워드] adopted antibody appearance appears attachment B cell B cells biological properties biological property can be used Cell cell-to-cell transmission characterization conformation conformational change convalescent COVID-19 patient convalescent COVID-19 patients convalescent patient convalescent patient serum Convalescent patients cytoplasmic demonstrated early stage early stages electron microscopy epitope Epitopes Evidence exhibited FIVE focus furin future therapeutic Golgi complex host cell Human human monoclonal antibodies Human monoclonal antibody immunofluorescence microscopy immunological indicated infected cells infected with SARS-CoV-2 Infection Interaction isolate isolates Microscopy mitigate MONITOR monoclonal new virus occur offer Protein provided RBD reagent Receptor binding domain receptor binding domain (RBD) recognize required S protein S protein cleavage S protein RBD SARS- CoV-2 SARS-CoV-2 SARS-CoV-2 clinical isolates SARS-CoV-2 spike SARS-CoV-2-infected cell SARS-CoV-2-infected cells Singapore Specific spike staining the RBD the S protein therapeutic These data Transmission Vaccine variant virus infection virus particles virus-infected cell virus-infected cells was used were used while [DOI] 10.1128/jvi.00455-22 PMC 바로가기 [Article Type] Article
The Translational Landscape of SARS-CoV-2-infected Cells Reveals Suppression of Innate Immune GenesSARS-CoV-2에 감염된 세포의 번역 환경은 선천 면역 유전자의 억제를 나타냅니다Article Published on 2022-06-282022-09-11 Journal: mBio [Category] COVID19(2023년), MERS, SARS, 유전자 메커니즘, 진단, [키워드] abundantly expressed acute respiratory syndrome acute respiratory syndrome coronavirus Air-liquid interface airway Cell cell cultures cell line cells cellular cellular mRNAs chemokine ciliated ciliated cell coding coronavirus cytokine downregulation event help highlight host mRNA host response host responses immune immune response immune responses infected cells Inflammatory Innate innate immune innate immune response Interaction interferon-stimulated genes involved landscape mechanism mediators modulate mRNA mRNA translation mRNAs notable primary cells programmed frameshifting regulated respiratory ribo-seq ribosomal frameshifting ribosome ribosome profiling ribosomes RNAs SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 replication SARS-CoV-2-infected cell SARS-CoV-2-infected cells sequence severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 suppression therapeutic therapeutic intervention These data transcriptional Transcriptional upregulation translated translation translation initiation translational translational repression unique upregulation Viral viral mRNA viral mRNAs virus virus replication Virus-host interaction [DOI] 10.1128/mbio.00815-22 PMC 바로가기 [Article Type] Article
Protocol to isolate and assess spike protein cleavage in SARS-CoV-2 variants obtained from clinical COVID-19 samplesArticle Published on 2022-06-132022-10-05 Journal: STAR protocols [Category] COVID19(2023년), SARS, 변종, 진단, 치료기술, [키워드] cell entry cellular proteases cleavage cleaved Complete COVID-19 sample ENhance Evolutionary biology facilitate in viral include Isolation Microbiology Nasopharyngeal swab patients with COVID-19 protocol SARS-CoV-2 SARS-CoV-2 spike protein SARS-CoV-2 variant SARS-CoV-2-infected cells spike mutation Spike protein supernatant [DOI] 10.1016/j.xpro.2022.101502 [Article Type] Article
Development of flow cytometry-based assays to assess the ability of antibodies to bind to SARS-CoV-2-infected and spike-transfected cells and mediate NK cell degranulationSARS-CoV-2 감염 및 스파이크 형질감염 세포에 결합하고 NK 세포 탈과립을 매개하는 항체의 능력을 평가하기 위한 유세포 분석 기반 분석법 개발Article Published on 2022-06-012022-09-11 Journal: Cytometry. Part A : the journal of the Internation [Category] COVID19(2023년), MERS, SARS, 진단, 치료기술, 치료제, [키워드] antibody Antibody binding Antibody Response Antibody responses Antibody titer Antibody titers assays binding capture Cell cells Clinical studies correlation COVID-19 degranulation demonstrated detect determine develop development Dilution dilution sery Efficiency effort elicited facilitate function functional antibody response healthy control high correlation Immunity IMPROVE infected cell infected cell. infected cells infected individuals magnitude moderate NK cell NK cell degranulation outcome overlap pandemic plasma platform ranged reduce response response rates responses SARS-CoV-2 SARS-CoV-2 disease SARS-COV-2 infection SARS-CoV-2 pandemic SARS-CoV-2-infected cell SARS-CoV-2-infected cells SARS-CoV-2-infected individual SARS-CoV-2-infected individuals Spearman the SARS-CoV-2 transfected cells Undetermined vaccination [DOI] 10.1002/cyto.a.24552 PMC 바로가기 [Article Type] Article
Anti-nucleocapsid antibodies enhance the production of IL-6 induced by SARS-CoV-2 N protein항-뉴클레오캡시드 항체는 SARS-CoV-2 N 단백질에 의해 유도된 IL-6의 생산을 향상시킵니다Article Published on 2022-05-162022-09-11 Journal: Scientific Reports [Category] COVID19(2023년), MERS, SARS, 바이오마커, 진단, 치료법, [키워드] acute respiratory distress acute respiratory distress syndrome acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addition antibody cause of death cell lysate coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 patient cultured macrophage cultured macrophages cytokine cytokine production Cytokine storm death disease ENhance IL-6 induce infected cells interleukin lysate macrophages mechanism myeloid cells N protein Patient patients Plasmid promote respiratory respiratory distress SARS-CoV-2 SARS-CoV-2 N protein SARS-CoV-2-infected cell SARS-CoV-2-infected cells Screening secreted sera severe acute respiratory syndrome Coronavirus syndrome the cell the cells the cytokine storm the disease [DOI] 10.1038/s41598-022-12252-y PMC 바로가기 [Article Type] Article
SARS-CoV-2 Nsp13 encodes for an HLA-E-stabilizing peptide that abrogates inhibition of NKG2A-expressing NK cellsSARS-CoV-2 Nsp13은 NKG2A 발현 NK 세포의 억제를 폐지하는 HLA-E 안정화 펩티드를 암호화합니다Article Published on 2022-03-082022-09-11 Journal: Cell Reports [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] abrogate activated acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Antigen binding Cell contribute coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 Defense detect ENCODE epithelial cells HLA-E Host host defense human leukocyte antigen immune cells in vitro infected cells infections inhibitory receptor innate immune cell Innate immune cells leukocyte limit lung epithelial cell mechanism missing self. natural NK cell NK cells NKG2A non-structural protein Patient patients with COVID-19 peptide Prevent Protein respond resulting SARS-CoV-2 SARS-CoV-2 replication SARS-CoV-2-infected cell SARS-CoV-2-infected cells severe acute respiratory syndrome Coronavirus susceptible target cell target cells These data virus [DOI] 10.1016/j.celrep.2022.110503 PMC 바로가기 [Article Type] Article
SARS-CoV-2 Infection Triggers Phosphorylation: Potential Target for Anti-COVID-19 TherapeuticsSARS-CoV-2 감염은 인산화를 유발합니다: Anti-COVID-19 치료제의 잠재적 표적Review Published on 2022-02-172022-09-11 Journal: Frontiers in Immunology [Category] 치료기술, 치료제, [키워드] Affect AKT autophagy change COVID-19 COVID-19 pandemic COVID-19 patient COVID-19 patients Drug discovery EIF2AK2 elevated enzyme function GFR Host infected cells kinase kinases MAPK mutations Phosphorylation phosphorylation site PIKfyve PKC Potential potential therapeutic target potential therapeutic targets Protein regulate responsible SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 viral SARS-CoV-2-infected cells Signaling signaling pathway signaling pathways specific inhibitor specific inhibitors survival target TGF-β Therapeutics Trigger triggers Viral proteins virus [DOI] 10.3389/fimmu.2022.829474 PMC 바로가기 [Article Type] Review