Recent Updates of the CRISPR/Cas9 Genome Editing System: Novel Approaches to Regulate Its Spatiotemporal Control by Genetic and Physicochemical StrategiesReview Published on 2024-06-062024-09-05 Journal: International journal of nanomedicine [Category] update2024, [키워드] clinical translation CRISPR/Cas9 Genome editing off-target effects sgRNA spatiotemporal control [DOI] 10.2147/IJN.S455574 PMC 바로가기 [Article Type] Review
Comparison of viral propagation and drug response among SARS-CoV-2 VOCs using replicons capable of recapitulating virion assembly and releaseArticle Published on 2022-10-012022-11-15 Journal: Virologica Sinica [Category] COVID19(2023년), SARS, 변종, 치료제, [키워드] acute respiratory syndrome assays Beta changed coronavirus Delta delta VoCs drug resistance drug response ENhance Evolution of SARS-CoV-2 genomes immune evasion inhibitor manifested Mutation novel antiviral drugs Protein provide quantification released remained Remdesivir replicon RNA S gene SARS-CoV-2 sgRNA supernatant Support Transcription variants variants of concern variants of concern (VOC) viral genome replication viral infectivity viral life cycle viral propagation Viral protein Viral-like particle (VLP). viral-like particles virion assembly VLP VoC VOCs WIV04 strain [DOI] 10.1016/j.virs.2022.06.008 PMC 바로가기
No infectious SARS-CoV-2 in breast milk from a cohort of 110 lactating womenArticle Published on 2022-10-012022-11-15 Journal: Pediatric Research [Category] COVID19(2023년), SARS, 치료기술, [키워드] addition analyzed Breast breast milk Breastfeeding breastmilk Cohort coronavirus Culture detectable diagnostic diagnostic tests Evidence examined experiments freeze-thaw cycles human milk Infants Infection Infectious virus lactating women marker occur positive recent remained risk factor RNA SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 RNA SARS-CoV-2 viral RNA sgRNA Symptom symptomatic tested Transmission viral culture virus vRNA women [DOI] 10.1038/s41390-021-01902-y PMC 바로가기
SARS-CoV-2 Virus Culture, Genomic and Subgenomic RNA Load, and Rapid Antigen Test in Experimentally Infected Syrian HamstersArticle Published on 2022-09-282022-11-16 Journal: Journal of Virology [Category] SARS, 유전자 메커니즘, [키워드] Antigen benefit characterized Contact tracing correlated COVID-19 COVID-19 patients Culture determine genomic genomic and subgenomic RNA genomic RNA gRNA hamster hamsters high correlation highlight Isolation load Nasal washes oropharyngeal swabs predicted Rapid rapid antigen test RNA RT-PCR SARS-CoV-2 sgRNA Syrian hamsters Test threshold viral culture virus [DOI] 10.1128/jvi.01034-22 PMC 바로가기
Detection and Kinetics of Subgenomic Severe Acute Respiratory Syndrome Coronavirus 2 RNA Viral Load in Longitudinal Diagnostic RNA-Positive SamplesArticle Published on 2022-09-132022-11-15 Journal: The Journal of Infectious Diseases [Category] SARS, 치료기술, [키워드] Above acute respiratory syndrome characterized collected coronavirus coronavirus 2 COVID-19 detectable detection diagnostic duration of illness identify independent Kinetics large sample linear load longitudinal nasal swab nucleic acid amplification participant peaking polymerase chain Postexposure Prophylaxis replicating virus Replication respiratory RNA RT-PCR Sample SARS-CoV-2 sgRNA sgRNAs subgenomic RNA swab sample Symptom threshold trajectory Treatment Viral load viral replication Viral RNA while [DOI] 10.1093/infdis/jiac048 PMC 바로가기
Detection of SARS-CoV-2 genomic and subgenomic RNA in retina and optic nerve of patients with COVID-19COVID-19 환자의 망막 및 시신경에서 SARS-CoV-2 게놈 및 서브게놈 RNA 검출Article Published on 2022-09-012022-09-11 Journal: The British journal of ophthalmology [Category] SARS, 유전자 메커니즘, 치료기술, [키워드] analysed Autopsy biopsy Blood Blood samples Case series COVID-19 Deceased detectable detection eye genomic humour immunohistochemistry Infection Microbiology molecular neuronal optic nerve Patient patients with COVID-19 Presence reported Retina RNA RNA load RNA loads SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 RNA SARS-CoV-2 spike protein sgRNA Spike protein throat swabs tissue unlikely Viral RNA viral RNA load virus cultivation virus isolation virus replication vitreous while [DOI] 10.1136/bjophthalmol-2020-318618 PMC 바로가기 [Article Type] Article
ChAdOx1 nCoV-19 (AZD1222) or nCoV-19-Beta (AZD2816) protect Syrian hamsters against Beta Delta and Omicron variantsChAdOx1 nCoV-19(AZD1222) 또는 nCoV-19-Beta(AZD2816)는 베타 델타 및 오미크론 변종으로부터 시리아 햄스터를 보호합니다.Article Published on 2022-08-082022-09-11 Journal: Nature Communications [Category] SARS, 변종, 진단, 치료기술, [키워드] Antibody titer AZD1222 Beta ChAdOx1 ChAdOx1 nCoV-19 clinical trials Delta delta variant Efficacy evaluate the effect full-length hamster hamsters Heterologous include inoculation lung Lungs Neutralizing antibody titer omicron post inoculation PROTECT Protective Protein real-world data reduced reduction in regimen S protein S proteins SARS-CoV-2 sequence sgRNA shown simian adenovirus-vectored vaccine single dose Substitution substitutions symptomatic disease the S protein vaccination Vaccine variant variants variants of concern vectored vaccine Viral virus VOCs Wuhan-1 [DOI] 10.1038/s41467-022-32248-6 PMC 바로가기 [Article Type] Article
Altered subgenomic RNA abundance provides unique insight into SARS-CoV-2 B.1.1.7/Alpha variant infections변경된 서브게놈 RNA 풍부도는 SARS-CoV-2 B.1.1.7/알파 변이체 감염에 대한 독특한 통찰력을 제공합니다Article Published on 2022-07-052022-09-11 Journal: Communications Biology [Category] COVID19(2023년), SARS, 변종, 유전자 메커니즘, 진단, 치료법, [키워드] Alpha Altered Antibody neutralization Applying ARTIC Network B.1.1.7 B.1.1.7 lineage B.1.177 Biology clinical samples Clinical severity complementary creating cycle threshold discontinuous transcription dominant lineage evaluate genomic genomic reads genomic sequencing greater hypothesise in vitro increase in independent independent of Infection Lineage lineages median Mutation nucleocapsid nucleotide Orf9b Oxford Nanopore Technology periscope positive produced profile protein level provide reductions in RNA sampling SARS-CoV-2 SARS-CoV-2 genome SARS-COV-2 infection SARS-CoV-2 infections sequence sgRNA significantly increased Support symptom onset the SARS-CoV-2 genome Transcription unique variant variants of concern virus [DOI] 10.1038/s42003-022-03565-9 PMC 바로가기 [Article Type] Article
Detectable Duration of Viable SARS-CoV-2, Total and Subgenomic SARS-CoV-2 RNA in Noncritically Ill COVID-19 Patients: a Prospective Cohort Study중증이 아닌 COVID-19 환자에서 생존 가능한 SARS-CoV-2, 전체 및 서브게놈 SARS-CoV-2 RNA의 감지 가능한 기간: 전향적 코호트 연구Article Published on 2022-06-292022-09-11 Journal: Microbiology Spectrum [Category] COVID19(2023년), MERS, SARS, 바이오마커, 치료기술, [키워드] 95% confidence interval acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Analysis clearance collected coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 Culture Determination determine diagnosed Duration Evidence Factor feasibility gold gold standard hazard ratio Ill Immunocompromised Immunocompromised patients infection control Infectivity interquartile range investigated Isolation Kinetics marker median time N gene Nasopharyngeal swab nasopharyngeal swabs negativity Patient Prospective prospective cohort receipt Reinfection respiratory reverse transcription PCR RNA RNA RT-PCR RT-PCR SARS-CoV-2 SARS-CoV-2 RNA SARS-CoV-2 sgRNA SARS-CoV-2 sgRNAs severe acute respiratory syndrome Coronavirus sgRNA sgRNAs steroid subgenomic RNA subgenomic RNAs surrogate markers time Total total RNA transcript Viable Viral viral culture viral infectivity Viral RNA viral shedding [DOI] 10.1128/spectrum.00503-22 PMC 바로가기 [Article Type] Article
Molecular Epidemiology of AY.28 and AY.104 Delta Sub-lineages in Sri Lanka스리랑카에서 AY.28 및 AY.104 델타 하위 계통의 분자 역학Article Published on 2022-06-212022-09-11 Journal: Frontiers in Public Health [Category] COVID19(2023년), SARS, 변종, 유전자 메커니즘, [키워드] A222V absence Analysis analyzed AY.104 AY.28 B.1.617.2 characterized clinical disease clinical implication clinical implications COVID-19 Delta delta variant determine dominant variant duration of hospitalization Epidemiology expression expression level genomic Illumina individual Infection investigated Laboratory Lineage lineages Mutation no difference ORF1a ORF3 ORF3a ORF6 outbreak Oxford parental periscope Prevalence RNA SARS-CoV-2 SARS-CoV-2 genome SARS-CoV-2 genomes sequenced Seven severe disease severity sgRNA significantly higher Spike protein sub genomic RNA sub lineages. the spike protein variant [DOI] 10.3389/fpubh.2022.873633 PMC 바로가기 [Article Type] Article