Comparison of Physical and Biochemical Characterizations of SARS-CoV-2 Inactivated by Different TreatmentsArticle Published on 2022-08-312022-11-15 Journal: Viruses [Category] SARS, 변종, 진단, [키워드] acute respiratory syndrome Affect affected aggregation Antigen biochemical biosafety caused characterization coronavirus diagnostics distress ELISA evaluate the effect formaldehyde immune inactivated inactivation Laboratory lack measure Microscope morphology no significant effect physical random Research S protein SARS-CoV-2 SARS-CoV-2 variant scattering secondary structure size exclusion chromatography specific treatment Spread surface plasmon resonance tested Treatment Vaccine Vaccine development Vaccines viral particle viral S protein virus virus inactivation α-helix β-propiolactone. β-sheet [DOI] 10.3390/v14091938 PMC 바로가기
A platform technology for generating subunit vaccines against diverse viral pathogensArticle Published on 2022-08-182022-11-15 Journal: Frontiers in Immunology [Category] 진단, [키워드] acting affinity tag animal model Antigen binding can be used Clamp conformation COVID-19 pandemic Ebola virus elicit epitope fusion fusion protein glycoprotein immune response immunologically Infectious disease MERS-CoV Microscopy middle molecular monoclonal antibody mRNA neutralising Neutralising Antibodies Nipah virus offer platform precursor prefusion purification reagent remained respiratory syndrome coronavirus shown size exclusion chromatography stability stimulated Streamline subunit Subunit vaccine subunit vaccines tested trimeric Vaccine vaccine candidate vaccine platform Viral Viral antigen viral challenge viral pathogen virus [DOI] 10.3389/fimmu.2022.963023 PMC 바로가기
Proteomic Profile of Procoagulant Extracellular Vesicles Reflects Complement System Activation and Platelet Hyperreactivity of Patients with Severe COVID-19응고촉진 세포외 소포의 단백질 프로파일은 중증 COVID-19 환자의 보체 시스템 활성화 및 혈소판 과민반응을 반영합니다Article Published on 2022-07-222022-09-11 Journal: Frontiers in Cellular and Infection Microbiology [Category] COVID19(2023년), SARS, 유전자 메커니즘, [키워드] acute inflammatory response alteration analyzed applied approach biological processe biological processes Biomarker Biomarkers cascades caused change changes in circulating Coagulation complement complement factors computational analysis COVID-19 COVID-19 patients Cytometry degranulation disease pathogenesis Diseases EVs experiment expressed extracellular vesicle Extracellular vesicles Flow flow cytometry group healthy donor healthy donors identify increase in indicated infected with SARS-CoV-2 LC-MS LC-MS/MS molecular pathophysiological Patient plasma Platelet Platelets procoagulant procoagulant activity profile progression Protein proteome proteomic SARS-CoV-2 SEC severe COVID-19 severe COVID-19 patients severity of COVID-19 significant changes in size exclusion chromatography System the disease tissue Tissue Factor total protein Vesicle was performed [DOI] 10.3389/fcimb.2022.926352 PMC 바로가기 [Article Type] Article
Human Basigin (CD147) Does Not Directly Interact with SARS-CoV-2 Spike GlycoproteinResearch Article Published on 2021-08-112022-10-30 Journal: mSphere [Category] COVID-19, MERS, SARS, [키워드] acute respiratory syndrome Basigin binding CD147 complex coronavirus coronavirus disease COVID-19 effective effort erythrocytes Escherichia coli expressed Factor full-length spike glycoprotein Health homolog Hospitalization Host host cells Human IgG IMPROVE Inclusion Infection Interaction Invasion ligands mammalian cells morbidity Mortality observation Plasmodium falciparum malaria polyclonal positive control preventing infection Protein Proteins RBD Receptor binding domain Replication reported SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 spike glycoprotein size exclusion chromatography spike the RBD the spike protein therapeutic target Treatment VERO E6 cells viral entry virus entry Virus-host interactions with COVID-19 [DOI] 10.1128/mSphere.00647-21 PMC 바로가기 [Article Type] Research Article
Transient and stabilized complexes of Nsp7, Nsp8, and Nsp12 in SARS-CoV-2 replicationSARS-COV-2 복제에서 NSP7, NSP8 및 NSP12의 과도 및 안정화 된 복합체Articles Published on 2021-08-032022-09-10 Journal: Biophysical Journal [Category] MERS, SARS, 유전자 메커니즘, [키워드] activities addition amplify atomic Combination complex complexation complexes component components conducted conformation Coordinate crystal structure crystal structures Crystallography defined Disruption DNA form functions greater high-resolution crystal structure Host host immune responses immune responses Interaction make up mechanism mRNA new virus Nsp12 Nsp7 NSP8 Propagation Protein Proteins recognizing Replication reported responsible RNA RNA-dependent RNA polymerase RTC SANS SARS-CoV-2 SARS-CoV-2 replication SAXS scattering size exclusion chromatography Structure Transcription Transient viability Viral Viral proteins Viral RNA virus [DOI] 10.1016/j.bpj.2021.06.006 PMC 바로가기 [Article Type] Articles
Enzymatic depolymerization of streptococcus pneumoniae type 8 polysaccharidearticle Published on 2020-09-302024-09-01 Journal: Clinical microbiology reviews [Category] 폐렴구균 감염증, [키워드] heparinase mass spectrometry NMR pneumococcal polysaccharide size exclusion chromatography Vaccine [Article Type] article
Effects of solution conditions on characteristics and size exclusion chromatography of pneumococcal polysaccharides and conjugate vaccinesarticle Published on 2016-11-302024-09-01 Journal: Carbohydrate Polymers [Category] 폐렴구균 감염증, [키워드] capsular polysaccharide ionic strength pneumococcus Protein-polysaccharide conjugates size exclusion chromatography Vaccines [Article Type] article
Genetic and antigenic characterization of recombinant nucleocapsid proteins derived from canine coronavirus and canine respiratory coronavirus in ChinaResearch Paper Published on 2016-04-152022-10-28 Journal: Science China. Life sciences [Category] Coronavirus, [키워드] Analysis antigenic antigenicity Antisera Beijing canine Canine coronavirus canine respiratory coronavirus China cloned conserved coronavirus Coronavirus infection cross-react cross-reactivity E. coli Escherichia coli expressed expression Final group HCoV-229E HKU1 human coronaviruses indicated mice N gene NL63 nucleocapsid protein OC43 polyacrylamide gel purified raised Respiratory Coronavirus SDS-PAGE sequenced size exclusion chromatography sodium dodecyl sulfate Swab virus were expressed [DOI] 10.1007/s11427-016-5038-1 PMC 바로가기 [Article Type] Research Paper
Kinetic plots in aqueous size exclusion chromatography of monoclonal antibodies and virus particlesArticle Published on 2015-12-242023-06-24 Journal: Journal of chromatography. A [Category] 신종인플루엔자, [키워드] Kinetic plots monoclonal antibodies particle size size exclusion chromatography Virions. [DOI] 10.1016/j.chroma.2015.11.057