Electrochemical paper-based antigen sensing platform using plant-derived monoclonal antibody for detecting SARS-CoV-2Article Published on 2023-01-012022-11-15 Journal: Talanta [Category] SARS, 진단, 치료기술, 치료제, [키워드] anti-SARS-CoV-2 antibody Antigen applicability applied approach Cellulose Concentration COVID-19 diagnosis CR3022 detect diagnostic Differential pulse voltammetry expressed functional group limit of detection linear relationship monoclonal antibody nasopharyngeal swab sample Nicotiana benthamiana Paper-based electrochemical immunosensor Plant-based antibody platform point-of-care pulse quantification RBD Receptor binding domain Saliva SAR-CoV-2 SARS-CoV-2 SARS-COV-2 infection Spike protein the RBD this system was performed [DOI] 10.1016/j.talanta.2022.123783 PMC 바로가기
A 1-year longitudinal study on COVID-19 convalescents reveals persistence of anti-SARS-CoV-2 humoral and cellular immunityCOVID-19 회복기에 대한 1년 종단 연구는 항 SARS-CoV-2 체액성 및 세포성 면역의 지속성을 보여줍니다Article Published on 2022-12-012022-09-11 Journal: Emerging Microbes & Infections [Category] COVID19(2023년), SARS, 변종, 진단, [키워드] 1-year after infection anti-SARS-CoV-2 humoral antibody antibody durability. caused CD4 CD8 CD8 T cell CD8 T cell responses Cell cellular immunity central memory circulating antibodies circulating antibody Cohort convalescent COVID-19 COVID-19 convalescent COVID-19 patient disease disease severity effective IgG level immune Immune components immune memory immune protection Immunity induce Infection information Kinetics maintain memory memory B memory B cell Memory responses memory T cells omicron Omicron variant peptide peptide pool persistence protective immunity provide RBD IgG Re-infection recorded response reveal SARS-CoV-2 SARS-CoV-2 circulating antibodies SARS-COV-2 infection subject Symptom T cell T cell response T cell responses T cells tested the RBD the SARS-CoV-2 variant [DOI] 10.1080/22221751.2022.2049984 PMC 바로가기 [Article Type] Article
Naturally occurring spike mutations influence the infectivity and immunogenicity of SARS-CoV-2Article Published on 2022-11-012022-11-15 Journal: Cellular and Molecular Immunology [Category] COVID19(2023년), SARS, 변종, 진단, [키워드] A570D A701V addition Affect affected antibodies antigenicity B.1.1.529 B.1.1.7 B.1.1.7 lineage B.1.351 B.1.617.2 BALB/c mice binding circulating cross-neutralization activity D1118H decrease Del Delta determine DNA vaccine Effects Efficiency elicited humoral immune response immune evasion immunized immunogen in vitro investigated K417N L18F Lineage Mutation NAbs Naturally neutralization Neutralizing Neutralizing antibodies neutralizing antibody NTD omicron opposite overcome P681H Pseudotyped viruses reduced S982A SARS-CoV-2 SARS-CoV-2 variant sera significantly increased single mutation single mutations spike mutation T cell T cell responses T-cell Response T716I tested the RBD Transmissibility unlikely Vaccine vaccine immunogenicity variants of concern viral entry viral infectivity VoC VOCs was determined weakened [DOI] 10.1038/s41423-022-00924-8 PMC 바로가기
Structural basis for mouse receptor recognition by SARS-CoV-2 omicron variantArticle Published on 2022-11-012022-11-16 Journal: Proceedings of the National Academy of Sciences of [Category] COVID19(2023년), SARS, 변종, [키워드] ACE2 acute respiratory syndrome Angiotensin-converting enzyme coronavirus COVID-19 domain Effect ENhance human ACE2 human Angiotensin-converting enzyme humans infect investigated mouse angiotensin-converting enzyme 2 Mutation Mutational hotspots N501Y Omicron variant question raised RBD receptor Receptor-binding domain (RBD) SARS-CoV-2 Spread the RBD viral adaptation X-ray X-ray crystallography [DOI] 10.1073/pnas.2206509119 PMC 바로가기
Identification of hACE2-interacting sites in SARS-CoV-2 spike receptor binding domain for antiviral drugs screeningArticle Published on 2022-11-012022-11-15 Journal: Virus research [Category] SARS, 변종, 치료제, [키워드] amino acid residue antiviral drug binding binding sites. Cefixime Ceftazidime cell entry Cephalosporin derivative clinical evaluation coronavirus 2 Critical derivative drug Drug screening example hACE2 human Angiotensin-converting enzyme identification Infection inhibited Interaction key amino acid live SARS-CoV-2 molecular docking Mutation peptide Pseudotyped virus pseudovirus RBD Receptor binding domain respiratory Safe SARS-CoV-2 SARS-CoV-2 spike SARS-CoV-2 Spike RBD Support targets tested the RBD the spike protein variants virus was determined wild type wildtype Wuhan [DOI] 10.1016/j.virusres.2022.198915 PMC 바로가기
Omicron (B.1.1.529) – A new heavily mutated variant: Mapped location and probable properties of its mutations with an emphasis on S-glycoproteinArticle Published on 2022-10-312022-11-16 Journal: International journal of biological macromolecules [Category] COVID19(2023년), SARS, 변종, [키워드] ACE2 receptor addition Analysis analyzed antibodies antibody Antibody binding B.1.1.529 bioinformatics characterized contribute country D614G eight human ACE2 receptor information investigated K417N mapped molecular mutated Mutation mutations N501Y NAb nAb escape. omicron outcome performed Quotient RBD RBD mutation recorded Region reported Research S477N SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2 VoC Seven spike spike mutation Spread statistical the binding affinity the RBD the SARS-CoV-2 variant while ΔΔG [DOI] 10.1016/j.ijbiomac.2022.07.254 PMC 바로가기
An antibody that neutralizes SARS-CoV-1 and SARS-CoV-2 by binding to a conserved spike epitope outside the receptor binding motifArticle Published on 2022-10-282022-11-15 Journal: Science Immunology [Category] COVID19(2023년), SARS, 변종, 진단, [키워드] ACE2 receptor acute respiratory syndrome antibody Beta binding conserved coronavirus cryo-EM structure Delta delta variant demonstrated develop DNA epitope Evolution glycosylation site identify immune lung mice neutralize neutralizing activities omicron RBD Receptor binding domain receptor binding motif reduced SARS Coronavirus SARS coronaviruses SARS-CoV-1 SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2-specific B cell sequenced single-cell the RBD therapeutic variant Viral load viral spike protein were infected [DOI] 10.1126/sciimmunol.abp9962 PMC 바로가기
Computational Design and Experimental Validation of ACE2-Derived Peptides as SARS-CoV-2 Receptor Binding Domain InhibitorsArticle Published on 2022-10-202022-11-16 Journal: The journal of physical chemistry. B [Category] COVID19(2023년), SARS, 변종, [키워드] ACE2 ACE2 binding Algorithm Alpha variant bind binding binding affinity candidate caused Concentration concentrations COVID-19 pandemic demonstrated disrupt domain ELISA assays entry of SARS-CoV-2 experiments expressed globe host cells human cells inhibitory initial insoluble Interaction moderate Molecular dynamics simulation molecule peptidase peptide Prevent Protein RBD SARS-CoV-2 SARS-CoV-2 RBD SARS-CoV-2-RBD sequence spike the RBD the SARS-CoV-2 therapeutic These data validation variants virus entry was used Wuhan-Hu-1 α1 helix [DOI] 10.1021/acs.jpcb.2c03918 PMC 바로가기
Evaluation of the systemic and mucosal immune response induced by COVID-19 and the BNT162b2 mRNA vaccine for SARS-CoV-2Article Published on 2022-10-182022-11-15 Journal: PLoS ONE [Category] COVID19(2023년), SARS, 변종, 진단, 치료기술, [키워드] antibody antibody production appear BNT162b2 BNT162b2 mRNA vaccine convalescent correlated COVID-19 COVID-19 patients decrease detectable eight elicit functional generate Heterologous humoral humoral immune response Immunity immunization increase induce marker mRNA vaccination mRNA vaccine mucosal mucosal immune NAb Neutralizing neutralizing capacity of BNT162b2 omicron peaked progression proportion protective immune response RBD RBD-binding antibody reduced Saliva SARS-CoV-2 second dose second vaccination sera significantly Spike protein subsequent the RBD the S1 subunit the SARS-CoV-2 vaccinated individual vaccination Vaccine vaccinee vaccinees variant virus neutralizing titers VoC VOCs wildtype [DOI] 10.1371/journal.pone.0263861 PMC 바로가기
Genetic diversity and evolutionary convergence of cryptic SARS- CoV-2 lineages detected via wastewater sequencingArticle Published on 2022-10-142022-11-15 Journal: PLoS Pathogens [Category] COVID19(2023년), SARS, 변종, 진단, 치료기술, 치료제, [키워드] Alpha VOC amino acid substitution amplify approach Course effective Epidemiology genetic diversity greater increasingly Infection Lineage lineages majority mutated New York City occurred omicron over Patient RBD Receptor binding domain reservoirs residue resolved SARS- CoV-2 SARS-CoV-2 SARS-CoV-2 surveillance sequence Sequencing Spread substitutions the RBD USA variant viral lineage VoC wastewater while [DOI] 10.1371/journal.ppat.1010636 PMC 바로가기