Potential pitfalls in analysing a SARS-CoV-2 RT-PCR assay and how to standardise data interpretationSARS-CoV-2 RT-PCR 분석 및 데이터 해석을 표준화하는 방법 분석의 잠재적 함정Article Published on 2022-10-012022-09-11 Journal: Journal of virological methods [Category] COVID19(2023년), SARS, 치료기술, [키워드] addition analysed Applied Biosystem Audit automated baseline Baseline threshold carried complex data interpretation determine Diagnostics.ai Frequency implementation in some increase in Interpretation introduced investigated Laboratory manual intervention Patient PCR positive result Potential processed produced provide Real-time PCR real-time RT-PCR relative Relative threshold RT-PCR RT-PCR kit RT-PCR kits SARS-CoV-2 SARS-CoV-2 RT-PCR solution tested thermocyclers threshold time-consuming virus detection was determined [DOI] 10.1016/j.jviromet.2022.114589 PMC 바로가기 [Article Type] Article
Emergency SARS-CoV-2 variants of concern: rapidly direct RT-qPCR detection without RNA extraction, clinical comparison, cost-effective, and high-throughput우려되는 긴급 SARS-CoV-2 변종: RNA 추출 없이 신속하게 직접 RT-qPCR 검출, 임상 비교, 비용 효율적 및 높은 처리량Article Published on 2022-06-022022-09-11 Journal: Aging (Albany NY) [Category] COVID19(2023년), SARS, 변종, 치료기술, [키워드] Accuracy acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 alternative method analytical sensitivity Chain Reaction characterized complex coronavirus coronavirus disease Coronavirus disease 2019 cost-effective diagnosis COVID-19 Diagnosis direct RT-qPCR dominant E gene Emergency Evolution faster gold gold standard High-throughput labor limit of detection mutations nasopharyngeal swabs Negative predictive value pandemic polymerase chain polymerase chain reaction positive Predictive value protocol real-time reverse transcriptase Real-time reverse transcriptase polymerase chain reaction reverse transcriptase Reverse transcriptase polymerase chain reaction RNA extraction RT-PCR RT-qPCR SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2 VoC sensitivity severe acute respiratory syndrome Coronavirus significantly Spike protein thermocyclers variant variants variants of concern [DOI] 10.18632/aging.204095 PMC 바로가기 [Article Type] Article
Efficient SARS-CoV-2 Quantitative Reverse Transcriptase PCR Saliva Diagnostic Strategy utilizing Open-Source Pipetting Robots오픈 소스 피펫팅 로봇을 활용한 효율적인 SARS-CoV-2 정량적 역전사효소 PCR 타액 진단 전략Article Published on 2022-02-112022-09-11 Journal: Journal of visualized experiments : JoVE [Category] COVID19(2023년), SARS, 치료기술, [키워드] automated Automation average Chain Reaction Clemson University clinical testing Community comparable complex Concentration COVID-19 pandemic diagnostic diagnostic testing efficient epidemiological handling Health Health crisis introduced invasive less Mortality multiplex RT-qPCR nasopharyngeal swabs nucleic acid nucleic acid testing Participants PCR physically polymerase chain polymerase chain reaction Population population surveillance Quantitative reagent Reporting Research reverse transcriptase robot RT-qPCR Saliva SARS-CoV-2 Sensitivity and specificity shown Strategy Supply chain Surveillance thermocycler thermocyclers transcriptase Transmission Viral RNA [DOI] 10.3791/63395 PMC 바로가기 [Article Type] Article
Evaluation of the Thermo Scientific SureTect Salmonella Species PCR Assay in a Broad Range of Foods and Select Environmental Surfaces: Pre-Collaborative and Collaborative Study: First Action 2021.02광범위한 식품 및 선별된 환경 표면에서 Thermo Scientific SureTect 살모넬라 종 PCR 분석 평가: 사전 협력 및 협력 연구: 첫 번째 조치 2021.02Article Published on 2022-02-042022-09-11 Journal: Journal of AOAC International [Category] SARS, 치료기술, [키워드] Action addition aims Amendment Analysis Applied Biosystem Applied Biosystems BAM comparable conducted Contamination correction COVID-19 pandemic cross-contamination demonstrated Department detection drug Egg Enumeration equivalence Europe evaluated extension false false-negative rate false-negative rates FDA fish food food safety highlighting identification indicate Inspection Laboratory laboratory result Laboratory results manual matrix Microbiology nine participant PCR performed poultry Probability produced product Range Rapid reagent reference method reported reproducibility robustness Safety Salmonella serotyping service Specy Sponge statistical analysis statistical model status tested the United State Thermo Thermo Scientific thermocycler thermocyclers United States US Food and Drug Administration [DOI] 10.1093/jaoacint/qsab122 PMC 바로가기 [Article Type] Article
Virus detection via programmable Type III-A CRISPR-Cas systems프로그래밍 가능한 Type III-A CRISPR-Cas 시스템을 통한 바이러스 탐지Article Published on 2021-09-272022-09-10 Journal: Nature Communications [Category] SARS, 변종, 진단, 치료기술, [키워드] activated activity amplification Assay systems assays cleavage complex conditions construction CRISPR-Ca CRISPR-Cas systems detect diagnosed disease detection dual enzyme flexible immune isothermal amplification nucleic acid Nucleic acid detection reached RNA SARS-CoV-2 SARS-CoV-2-infected patient sensitivity thermocyclers this system Transcription Type type III unique Viral Viral RNA virus detection [DOI] 10.1038/s41467-021-25977-7 PMC 바로가기 [Article Type] Article
Detection of SARS-CoV-2 RNA using RT-LAMP and molecular beaconsRT-LAMP 및 분자 표지를 사용한 SARS-CoV-2 RNA 검출Research Published on 2021-06-032022-09-11 Journal: Genome Biology [Category] MERS, SARS, 치료기술, [키워드] alternative Amplicon amplification assays Chain Reaction complicate Concordance coronavirus COVID-19 detection Diagnosis DNA amplification facilitate fluorescent global pandemic gold gold standard human RNA implementation incorporation isothermal amplification LAMP LAMP-BEAC less Loop-mediated isothermal amplification melting temperatures molecular Molecular beacon monomer multiplex nonspecific nucleic acid polymerase polymerase chain reaction Population screening provide purification Rapid rapid assay rapid assays Reactions reagent Result resulting reverse transcription Reverse transcription-polymerase chain reaction robust RT-LAMP RT-PCR RT-qPCR Saliva saliva samples SARS-CoV-2 SARS-CoV-2 genome SARS-CoV-2 genomes SARS-CoV-2 RNA sensitivity Spread supplementary material supply chains thermocyclers Transcription Transmission [DOI] 10.1186/s13059-021-02387-y PMC 바로가기 [Article Type] Research
Validation of a single-step, single-tube reverse transcription loop-mediated isothermal amplification assay for rapid detection of SARS-CoV-2 RNAEditor's Choice Published on 2020-09-052022-10-29 Journal: Journal of Medical Microbiology [Category] COVID-19, SARS, [키워드] alleviate average bypassing CDC clinical sample Combination conducted COVID-19 detect diagnostic diagnostics Dilution E-gene enzyme evaluated FAM FDA implementation intervals isothermal amplification Laboratory medium Nasopharyngeal swab nasopharyngeal swabs nucleocapsid primer required resource Result reverse transcription RNA extraction kit RNA purification RT-LAMP RT-qPCR SARS-CoV-2 SARS-CoV-2 pandemic SARS-CoV-2 RNA SARS-CoV-2 virus screened Sensitivity and specificity specimen TCID the SARS-CoV-2 thermocycler thermocyclers Transport tube universal transport media UTM validation virus detection [DOI] 10.1099/jmm.0.001238 PMC 바로가기 [Article Type] Editor's Choice