Human surfactant protein D facilitates SARS-CoV-2 pseudotype binding and entry in DC-SIGN expressing cells, and downregulates spike protein induced inflammation인간 계면활성제 단백질 D는 DC-SIGN 발현 세포에서 SARS-CoV-2 유사형 결합 및 진입을 촉진하고 스파이크 단백질 유도 염증을 하향 조절합니다Article Published on 2022-07-282022-09-11 Journal: Frontiers in Immunology [Category] COVID19(2023년), SARS, 유전자 메커니즘, 치료기술, [키워드] ACE2 Analysis Anti-viral binding carbohydrate cause Cell cell interaction cell line cell surface receptor cells chemokine chemokines collectins controls COVID-19 cytokine cytokine response Cytokines DC-SIGN dendritic cell domain downregulate downregulated eight epithelial expressing facilitate fragment of HEK 293T HEK293T cell HEK293T cells help Human IFN-α IL- 6 IL-1β IL-8 immune function immune response immunomodulatory effect increase independent independent of infection assay infection assays Inflammation inhibit SARS-CoV-2 innate immune innate immune system Interaction intercellular involved Macrophage macrophages MHC class MHC class II modulating mRNA expression mRNA level mRNA levels N-terminal naïve T cell naïve T cells NF-κB pathogen Pathogens pro-inflammatory cytokine pro-inflammatory cytokines Protein pseudotype RANTES receptors Replication rfhSP-D. RNA virus RT-qPCR SARS- CoV-2 SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 replication SARS-CoV-2 spike protein shown SIGN spike Spike protein surface receptor T cells the Spike THP-1 TNF-α treated Treatment Vero Cell Vero cells viral entry viruses [DOI] 10.3389/fimmu.2022.960733 PMC 바로가기 [Article Type] Article
Cyanidin-3-O-glucoside and Peonidin-3-O-glucoside-Rich Fraction of Black Rice Germ and Bran Suppresses Inflammatory Responses from SARS-CoV-2 Spike Glycoprotein S1-Induction In Vitro in A549 Lung Cells and THP-1 Macrophages via Inhibition of the NLRP3 Inflammasome Pathway시아니딘-3-O-글루코사이드 및 피오니딘-3-O-글루코사이드-풍부 검정 쌀 배아 및 겨 분획은 A549 폐 세포 및 THP-1 대식세포에서 SARS-CoV-2 스파이크 당단백질 S1-유도 시험관 내 염증 반응을 억제합니다 NLRP3 Inflammasome 경로의 억제Article Published on 2022-06-302022-09-11 Journal: Nutrients [Category] COVID19(2023년), SARS, 유전자 메커니즘, [키워드] A549 Activation anthocyanin anthocyanins Anti-inflammation anti-inflammatory property ASC attenuate benefit black black rice bran C3G Cell cell lines Concentration counteraction COVID-19 COVID-19 infection cytokine secretion dose-dependent manner downregulation Effect exhibited exposure to fraction functional functional food Gene Expression Germ Health health benefits HPLC IL-18 IL-1β IL-6 immune cells in vitro model Inflammasome Inflammation Inflammatory Inflammatory response Inflammatory responses inhibition innate immune cell Innate immune cells long-COVID lung tissue Macrophage macrophages mechanism NF-kB NLRP3 NLRP3 inflammasome P3G pathway post-acute COVID protective effect Protective effects Protein protein level protein levels reported response responsible Rice S1 subunit SARS-CoV-2 spike spike glycoprotein spike glycoprotein S1. THP-1 treated was determined [DOI] 10.3390/nu14132738 PMC 바로가기 [Article Type] Article
AMP5A modulates Toll-like receptors 7 and 8 single-stranded RNA immune responses in PMA-differentiated THP-1 and PBMCResearch Published on 2022-03-032022-10-29 Journal: Translational Medicine Communications [Category] COVID-19, [키워드] activate activated Activation acute respiratory syndrome addition agonist AhR AMP5A And Chemokines antagonist antiviral immunity Aryl hydrocarbon receptor Autoimmunity biologic chemokines clinical investigation condition coronavirus coronavirus disease Course COVID-19 CXCL10 cytokine Cytokines decrease detect differentially expressed dysregulated Effect ELISA enrichment analysis evaluate experiment fraction Human serum albumin hyperinflammation Hyperinflammatory IL-12 IL-1β IL-6 Imbalance Immune cell immune response immunomodulatory implicated Infection Inflammation inflammatory mediator Inflammatory response inhibit Intervention involved low molecular weight Lung disease mechanism modulate mononuclear cells myeloid cell myeloid cells NF-κB NF-κB activation Osteoarthritis pathway PBMC Peripheral blood PPARγ pro-inflammatory pro-inflammatory cytokine profiles progression Protective provided receptor REP Result resulting RNA Severe case Signaling single-stranded ssRNA suppresse therapeutic approach THP-1 TLR TLR7 TLR7/8 Toll-like receptor toll-like receptor 7 Toll-like receptor 8 transcription factor transcription factors treat Treatment viral sequences were used with COVID-19 [DOI] 10.1186/s41231-022-00110-y PMC 바로가기 [Article Type] Research
Instant determination of the artemisinin from various Artemisia annua L. extracts by LC-ESI-MS/MS and their in-silico modelling and in vitro antiviral activity studies against SARS-CoV-2LC-ESI-MS/MS 및 SARS-CoV-2에 대한 in-silico 모델링 및 시험관 내 항바이러스 활성 연구에 의한 다양한 Artemisia annua L. 추출물의 artemisinin의 즉각적인 측정Article Published on 2022-03-012022-09-11 Journal: Phytochemical analysis : PCA [Category] SARS, 치료제, [키워드] 3CL 50% effective concentration additional treatment option Antiviral antiviral activity applied Artemisia annua SARS-CoV-2 Artemisinin binding biological activity can be used Cell cell viability COVID-19 COVID-19 treatment determine Direct dose effort energy transfer enzyme activity greater HEK293T cell line immunosuppressive effect immunosuppressive effects in silico in vitro in-silico LC-ESI-MS/MS lipopolysaccharide Liquid chromatography mass spectrometry Medicines molecular natural products for COVID-19. Numerous patients plant Prophylactic protease Protease activity pseudovirus reported resolved SARS-CoV-2 SARS-CoV-2 virus spike Spike protein Tandem mass spectrometry THP-1 TNF-α Treatment Turkey viability [DOI] 10.1002/pca.3088 PMC 바로가기 [Article Type] Article
Elimination of Aicardi–Goutières syndrome protein SAMHD1 activates cellular innate immunity and suppresses SARS-CoV-2 replicationAicardi-Goutières 증후군 단백질 SAMHD1 제거는 세포 선천 면역을 활성화하고 SARS-CoV-2 복제를 억제합니다Research Article Published on 2022-01-252022-09-10 Journal: The Journal of Biological Chemistry [Category] MERS, SARS, 치료제, [키워드] 293T ACE2, angiotensin-converting enzyme 2 activate activated Activation acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Affect AGS, Aicardi–Goutières syndrome Antiviral ARHGEF5, Rho guanine nucleotide exchange factor 5 Baricitinib cDNA, complementary DNA Cell cell lines cells cellular characterized clearance coronavirus COVID-19, Coronavirus disease 2019 CRISPR Culture Degradation demonstrated DMEM, Dulbecco's modified Eagle's medium downregulated elevated Elimination expression FBS, fetal bovine serum FDA, US Food and Drug Administration Gene Expression Genetic HCoV-OC43, human coronavirus OC43 Human coronavirus human coronavirus OC43 IFN IFN signaling IFN, interferon IFNs immune responses Infection innate immune response Innate immunity interferon interferons interferons (IFNs) IRF3, IFN regulatory factor 3 ISG, IFN-stimulated gene JAK inhibitor JAK pathway JAK, Janus kinase lack lentiviral Macrophage macrophages MDM, monocyte-derived macrophage model system MOI, multiplicity of infection Monocyte-derived macrophages mRNA NSP, nonstructural protein Pathways Phosphorylation Protein protein expression pSTAT1, phosphorylated form of STAT1 qRT–PCR, quantitative RT–PCR regulate Replication respiratory SAMHD1 SAMHD1, sterile alpha motif and histidine–aspartate domain–containing protein 1 SARS-CoV-2 SARS-CoV-2 replication SARS-CoV-2 RNA SARS-CoV-2–infected patient SARS-CoV-2–infected patients SARS-CoV-2, Severe acute respiratory syndrome coronavirus 2 severe acute respiratory syndrome Coronavirus shown Signaling signaling pathway significantly increased Stat1 STAT1, signal transducer and activator of transcription 1 suppresse suppressed syndrome THP-1 tissue tissue culture Treatment upregulated Viral Viral protein Virus-like particle virus-like particles VLP, virus-like particle Vpx, viral protein X [DOI] 10.1016/j.jbc.2022.101635 PMC 바로가기 [Article Type] Research Article
Specific inhibition of the NLRP3 inflammasome suppresses immune overactivation and alleviates COVID-19 like pathology in miceNLRP3 인플라마좀의 특정 억제는 면역 과잉 활성화를 억제하고 생쥐의 병리와 같은 COVID-19를 완화합니다Article Published on 2021-12-312022-09-11 Journal: EBioMedicine [Category] SARS, 유전자 메커니즘, 진단, [키워드] activated Activation advance alleviate biosafety level BSL-3 Bureau C57BL/6 CAS Cell culture cell cultures conducted coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 disease COVID-19 severity Cytokines education Evidence finding funding global public health histological host gene Host immune response human ACE2 immune immunofluorescence analysis immunopathology indicated inhibition inhibitor injected Intervention intranasally intraperitoneally Invasion involved knockout mice Laboratory level 3 live SARS-CoV-2 Lung inflammation lung tissue lung tissues Lungs macrophages MCC950 mice NLRP3 NLRP3 inflammasome pandemic pathology performed pro-inflammatory cytokine pro-inflammatory cytokines Program provided quantitative real-time PCR SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2, NLRP3 inflammasome, Immune overactivation, Cytokine storm Science severe COVID-19 disease severe COVID-19 patient severe COVID-19 patients severe pathology severity Specific supported suppresse targeting THP-1 tissues transgenic mice Vaccine development Viral was reduced West wild-type with COVID-19 [DOI] 10.1016/j.ebiom.2021.103803 PMC 바로가기 [Article Type] Article
SARS-CoV-2 Spike Protein S1-Mediated Endothelial Injury and Pro-Inflammatory State Is Amplified by Dihydrotestosterone and Prevented by Mineralocorticoid AntagonismArticle Published on 2021-11-032022-10-28 Journal: Viruses [Category] COVID-19, MERS, SARS, [키워드] abrogated Activation acute respiratory syndrome Adhesion Molecule affected androgen androgen signaling angiotensin receptor blocker antagonism Cell circulating condition coronavirus Coronavirus-2 COVID-19 death defined diagnosed with COVID-19 Dihydrotestosterone E-selectin Effect endothelial Endothelial cell endothelial injury exacerbated exposure FDA-approved drug higher odd ICAM-1 in vitro Inflammatory cytokine marker men men and women Mineralocorticoid monocyte Necrosis patients hospitalized receptor reduced SARS-CoV-2 SARS-CoV-2 spike spike Spike protein spironolactone state tested therapeutic effect THP-1 TNF-α transcript Treatment vascular effect VCAM-1 were measured with COVID-19 women [DOI] 10.3390/v13112209 PMC 바로가기 [Article Type] Article
SARS-CoV-2 Antibodies Mediate Complement and Cellular Driven InflammationSARS-CoV-2 항체는 보체 및 세포 유발 염증을 매개합니다Immunology Published on 2021-11-022022-09-12 Journal: Frontiers in Immunology [Category] MERS, SARS, 진단, [키워드] Activation acute respiratory syndrome Antibody Response C1q caused cell line cellular characterized classical Combination complement Complement pathway component consequence convalescent individual coronavirus coronavirus disease correlated COVID-19 COVID-19 disease COVID-19 disease severity COVID-19 severity disease severity distribution domain driven by Effect ELISAs exacerbating IgG IgG level IgG response IgG subclasse IgG1 IgG3 individual Inflammatory response Ligand monocyte Monocytes natural infection outcome pandemic plasma priming pro-inflammatory cytokine public health threat question RBD receptor Receptor binding domain receptor engagement risk SARS-CoV-2 SARS-CoV-2 antibody SARS-COV-2 infection SARS-CoV-2 specific antibodies SARS-CoV-2 specific antibody secretion severe disease significantly spike subsequent the antibody response the SARS-CoV-2 THP-1 TNF-α viral infection viral neutralization were used [DOI] 10.3389/fimmu.2021.767981 PMC 바로가기 [Article Type] Immunology
Synthesis of novel calcium channel blockers with ACE2 inhibition and dual antihypertensive/anti-inflammatory effects: A possible therapeutic tool for COVID-19ACE2 억제 및 이중 항고혈압/항염 효과를 갖는 신규 칼슘 채널 차단제 합성: COVID-19에 대한 가능한 치료 도구Article Published on 2021-11-012022-09-11 Journal: Bioorganic chemistry [Category] SARS, 치료제, [키워드] ACE2 ACE2 binding ACE2 receptor activity addition Anti-inflammatory anti-inflammatory effect antihypertensive agent Bioisosters blocker blocking activity calcium calcium channel CCB CCBs cells Comorbidity Compound consequence COVID-19 COVID-19 infection COVID-19 patients CRP Efficacy evaluate evaluated exhibited highest hypertension IL-6 inhibitory effect MOST Nifedipine protective effect pyrimidines RBD reference standard researcher risk factor Risk factors SARS-CoV-2 SARS-CoV-2 spike SARS-CoV-2 Spike RBD screened seriousness spike suppression synthesis tested therapeutic THP-1 THP‐1 cells. [DOI] 10.1016/j.bioorg.2021.105272 PMC 바로가기 [Article Type] Article
Increased LPS levels coexist with systemic inflammation and result in monocyte activation in severe COVID-19 patients증가된 LPS 수준은 전신 염증과 공존하며 심각한 COVID-19 환자에서 단핵구 활성화를 초래합니다Article Published on 2021-11-012022-09-12 Journal: International immunopharmacology [Category] MERS, SARS, 진단, [키워드] Activation alteration analyzed association Bacterial blood sample CCL2/MCP-1 CCR2 CCR5 CCR7 CD14 CD16 CD69 Cell cell line Chemokine receptor chemokines collected Concentration Control controls COVID-19 COVID-19 patient COVID-19 patients Cytokines dead death Diseases evaluate expression flow cytometry Hospital admission hospital discharge Hospitalization hyperinflammation IFN-γ IL-6 Increased incubated Inflammation LPS marker microbial microbial translocation monocyte Monocyte activation mucosal barrier nine Non-COVID-19 non-survivor outcomes Pathogenesis Patient phenotype plasma Pneumonia positive patient SARS-CoV-2 SARS-CoV-2 RT-PCR SARS-CoV-2-infected patients SARS-CoV-2. severe COVID-19 severe COVID-19 patient survivor systemic cytokine systemic inflammation TGF-β1 THP-1 TLR4 TNF-α translocation worsening [DOI] 10.1016/j.intimp.2021.108125 PMC 바로가기 [Article Type] Article