tissue culture – Communicable Diseases Research

A SCID Mouse Model To Evaluate the Efficacy of Antivirals against SARS-CoV-2 Infection

SARS-CoV-2 감염에 대한 항바이러스제의 효능을 평가하기 위한 SCID 마우스 모델

Article

Published on 2022-08-242022-09-11
Journal: Journal of Virology [Category] SARS, 변종, 치료제,

Differential susceptibility to SARS-CoV-2 in the normal nasal mucosa and in chronic sinusitis

정상 비점막 및 만성 부비동염에서 SARS-CoV-2에 대한 감수성 감수성

Article

Published on 2022-08-012022-09-11
Journal: European Journal of Immunology [Category] COVID19(2023년), SARS, 유전자 메커니즘,

[키워드] ACE2 ACE2-expressing cell ACE2-expressing cells acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 antiviral immune response association Brain chronic Chronic rhinosinusitis close monitoring coronavirus CRS Culture deficiency disease eosinophils Eosinophils Human nasal mucosa Evidence gene expression difference gene expression differences high risk Human increase in individuals Infection investigated lung mechanism nasal nasal mucosa other organ other organs Patient progression reservoir respiratory tract rhinosinusitis SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 viral infection severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 Subtypes suggested susceptibility susceptible tissue tissue culture Transmission viral infection viral replication
[DOI] 10.1002/eji.202249805 PMC 바로가기 [Article Type] Article

Detection of SARS-CoV-2 virus via dynamic light scattering using antibody-gold nanoparticle bioconjugates against viral spike protein

바이러스 스파이크 단백질에 대한 항체-금 나노입자 생체접합체를 사용한 동적 광산란을 통한 SARS-CoV-2 바이러스 검출

Article

Published on 2022-06-012022-09-11
Journal: Talanta [Category] COVID19(2023년), SARS, 치료기술,

Between Simians and Cell Lines: Rhesus Monkeys, Polio Research, and the Geopolitics of Tissue Culture (1934-1954)

article

Published on 2022-03-312024-09-01
Journal: Case Reports in Cardiology [Category] 폴리오,

[키워드] Empire Geopolitics nonhuman primates Polio research South Asia tissue culture
[Article Type] article

Exploration of the ocular surface infection by SARS-CoV-2 and implications for corneal donation: An ex vivo study

SARS-COV-2에 의한 안구 표면 감염의 탐색 및 각막 기증에 대한 시사점 : 생체 외 연구

Research Article

Published on 2022-03-012022-09-10
Journal: PLoS Medicine [Category] MERS, SARS, 치료기술,

[키워드] 24 hour 24 hours ACE-2 acute respiratory syndrome aims Algorithms angiotensin angiotensin-converting enzyme 2 bulbar conjunctiva cathepsin cellular entry Conjunctiva Contamination contraindication contraindications Cornea Corneal Corneal Graft Corneal transplantation coronavirus coronavirus 2 coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 crisis Ct value Culture culture media decrease diagnosed diagnosed with COVID-19 Donor Donor selection donors enzyme epithelial cell epithelial cells epithelium evaluate Evidence Ex vivo exacerbated exploration expresse expressed expression expression of SARS-CoV-2 expression pattern female genetic material healthy donor immunohistochemistry implication IMPROVE increase in Infection infectious dose inoculation involved lack limbus limited sample size mandatory media median moderate molecular mRNA level nasopharyngeal Nasopharyngeal swab nasopharyngeal swabs Not contaminated Ocular surface organ organ culture pandemic pathway Patient PCR positive Positive test Postinfection promote promoted Protein qPCR quantified quantitative PCR question RdRP receptor Replication researcher respiratory respond retrieved reverse transcription Reverse transcription quantitative PCR risk risk of transmission RNA RNA copy RNA sequence RT-qPCR SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 receptor SARS-CoV-2 receptors SARS-CoV-2 RNA SARS-CoV-2 viral SARS-CoV-2 virus Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 significant increase Support TCID tested tissue tissue culture tissues TMPRSS2 Transmission utility Viral Viral detection Viral RNA was tested were infected worsened
[DOI] 10.1371/journal.pmed.1003922 PMC 바로가기 [Article Type] Research Article

Photothermal inactivation of universal viral particles by localized surface plasmon resonance mediated heating filter membrane

국소화 된 표면 플라즈몬 공명 매개 가열 필터 멤브레인에 의한 보편적 바이러스 입자의 광열 불 활성화

Article

Published on 2022-02-022022-09-01
Journal: Scientific Reports [Category] COVID19(2023년), MERS, SARS, 진단,

[키워드] 50% tissue culture infective dose cells Chain Reaction coated components Crystal violet Culture deformation denaturation dose effective gold H1Npdm09 HFM inactivation infectious sample infectious samples infective Infective dose Influenza virus irradiation Mardin membrane membrane protein Membrane proteins Nanoparticles Nanophotonics and plasmonics Natural hazards polymerase chain polymerase chain reaction provide qRT-PCR Quality control Quantitative reduce reduction reduction in reverse transcription room temperature SARS-CoV-2 staining surface plasmon surface plasmon resonance surface temperature TCID temperature tissue tissue culture Viral viral gene viral genes viral infectivity viral particle virus titer was reduced
[DOI] 10.1038/s41598-022-05738-2 PMC 바로가기 [Article Type] Article

Elimination of Aicardi–Goutières syndrome protein SAMHD1 activates cellular innate immunity and suppresses SARS-CoV-2 replication

Aicardi-Goutières 증후군 단백질 SAMHD1 제거는 세포 선천 면역을 활성화하고 SARS-CoV-2 복제를 억제합니다

Research Article

Published on 2022-01-252022-09-10
Journal: The Journal of Biological Chemistry [Category] MERS, SARS, 치료제,

[키워드] 293T ACE2, angiotensin-converting enzyme 2 activate activated Activation acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Affect AGS, Aicardi–Goutières syndrome Antiviral ARHGEF5, Rho guanine nucleotide exchange factor 5 Baricitinib cDNA, complementary DNA Cell cell lines cells cellular characterized clearance coronavirus COVID-19, Coronavirus disease 2019 CRISPR Culture Degradation demonstrated DMEM, Dulbecco's modified Eagle's medium downregulated elevated Elimination expression FBS, fetal bovine serum FDA, US Food and Drug Administration Gene Expression Genetic HCoV-OC43, human coronavirus OC43 human coronavirus human coronavirus OC43 IFN IFN signaling IFN, interferon IFNs immune responses Infection innate immune response Innate immunity interferon interferons interferons (IFNs) IRF3, IFN regulatory factor 3 ISG, IFN-stimulated gene JAK inhibitor JAK pathway JAK, Janus kinase lack lentiviral Macrophage macrophages MDM, monocyte-derived macrophage model system MOI, multiplicity of infection Monocyte-derived macrophages mRNA NSP, nonstructural protein Pathways Phosphorylation Protein protein expression pSTAT1, phosphorylated form of STAT1 qRT–PCR, quantitative RT–PCR regulate Replication respiratory SAMHD1 SAMHD1, sterile alpha motif and histidine–aspartate domain–containing protein 1 SARS-CoV-2 SARS-CoV-2 replication SARS-CoV-2 RNA SARS-CoV-2–infected patient SARS-CoV-2–infected patients SARS-CoV-2, Severe acute respiratory syndrome coronavirus 2 severe acute respiratory syndrome Coronavirus shown Signaling signaling pathway significantly increased Stat1 STAT1, signal transducer and activator of transcription 1 suppresse suppressed syndrome THP-1 tissue tissue culture Treatment upregulated Viral Viral protein Virus-like particle virus-like particles VLP, virus-like particle Vpx, viral protein X
[DOI] 10.1016/j.jbc.2022.101635 PMC 바로가기 [Article Type] Research Article

Air-Liquid-Interface Differentiated Human Nose Epithelium: A Robust Primary Tissue Culture Model of SARS-CoV-2 Infection

공기-액체-인터페이스 분화된 인간 코 상피: SARS-CoV-2 감염의 강력한 1차 조직 배양 모델

Article

Published on 2022-01-132022-09-10
Journal: International Journal of Molecular Sciences [Category] 변종,

[키워드] air–liquid-interface apical surface basal cells caused Cell cell damage ciliated cells communicable communicable disease condition confocal microscopy COVID-19 COVID-19 pandemic Culture culture system delta variant determined by Differentiated cell disease dominant Donor donors Effectiveness feature Human human nasal epithelium human SARS-CoV-2 human tissue human tissues immunofluorescence infected cell infected cells Infection initial inoculated invasive medical countermeasure Medical countermeasures Model nasal nasal epithelium nasal turbinate nose occurred offer organoids portal Prevent progenitor progenitor cells replicate respiratory respiratory tissue robust SARS-CoV-2 SARS-CoV-2 clinical isolates SARS-COV-2 infection Severe acute respiratory syndrome TCID tissue tissue culture unmet need urgency Viral viral entry virus was determined
[DOI] 10.3390/ijms23020835 PMC 바로가기 [Article Type] Article

Analytical Performance of Quantitative DiaSorin Liaison SARS-COV-2 Antigen Test for the Asymptomatic Population

무증상 집단에 대한 정량적 DiaSorin 연락 SARS-COV-2 항원 테스트의 분석 성능

Public Health

Published on 2022-01-072022-09-11
Journal: Frontiers in Public Health [Category] MERS, SARS, 치료기술,

[키워드] Antigen antigen detection Asymptomatic asymptomatic patients automated chemiluminescence conducted coronavirus coronavirus 2 correlation COVID-19 infection Cutoff cycle threshold Diagnosis DiaSorin evaluate exceeded facilitate flexibility group identify material milliliter molecular monitoring population nasal swab nasal swabs negative sample NPs pandemic Patient performance Population positive Rapid reached respiratory Retrospective study reverse transcriptase risk RT-PCR Sample SARS-CoV-2 sensitivity Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 specificity TCID Test tested tissue culture Tissue Culture Infectious Dose Variability Viral Viral load
[DOI] 10.3389/fpubh.2021.788581 PMC 바로가기 [Article Type] Public Health

Safety and immunogenicity of a measles-vectored SARS-CoV-2 vaccine candidate, V591 / TMV-083, in healthy adults: results of a randomized, placebo-controlled Phase I study

건강한 성인에서 홍역 매개 SARS-CoV-2 백신 후보 V591/TMV-083의 안전성 및 면역원성: 무작위, 위약 대조 1상 연구 결과

Clinical Trial

Published on 2022-01-012022-09-11
Journal: EBioMedicine [Category] SARS, 임상, 치료기술,

[키워드] adverse event adverse events age antibodies antibody Belgium binding antibodies Bioscience CEPI Chikungunya Coalition cohorts COVID-19 detectable dose Dose escalation double-blind evaluate expressing France healthy High dose high dose vaccine immune response Immunity immunogenicity Inc. injection Injections Innovation Intramuscular injection low dose low dose vaccine measles vector median Neutralizing antibodies neutralizing antibody participant performed phase Placebo placebo recipients placebo-controlled pre-fusion Randomized receiving reported responses Safety safety profile SARS-CoV-2 SARS-CoV-2 spike SARS-CoV-2 spike protein SARS-CoV-2 vaccine screened Serious Adverse Event Serious Adverse Events Spike protein spike-specific T cell statistically significant subsidiary Support T cell T cell response T cell responses TCID THEMIS tissue culture treatment phase Trial Vaccine vaccine candidate vaccinee Volunteer was measured while
[DOI] 10.1016/j.ebiom.2021.103810 PMC 바로가기 [Article Type] Clinical Trial