A methodological primer of extracellular vesicles isolation and characterization via different techniquesMethods Article Published on 2024-02-132024-09-05 Journal: Biology Methods & Protocols [Category] update2024, [키워드] Exosome extraction PEG 8000 Polymer ultracentrifugation Ultrafiltration [DOI] 10.1093/biomethods/bpae009 PMC 바로가기 [Article Type] Methods Article
Congestion in Heart Failure: From the Secret of a Mummy to Today’s Novel Diagnostic and Therapeutic Approaches: A Comprehensive ReviewReview Published on 2023-12-192024-09-05 Journal: Journal of Clinical Medicine [Category] update2024, [키워드] acute decompensated heart failure diuretic resistance fluid overload loop diuretics Mineralocorticoid SGLT2 inhibitors Ultrafiltration [DOI] 10.3390/jcm13010012 PMC 바로가기 [Article Type] Review
Efavirenz-Based Antiretroviral Therapy but Not Pregnancy Increased Unbound Piperaquine Exposure in Women during Malaria ChemopreventionArticle Published on 2023-04-182024-08-28 Journal: Med Instrum [Category] 말라리아, [키워드] drug-drug interaction efavirenz malaria Pregnancy Ultrafiltration unbound piperaquine.
Development of an efficient wastewater testing protocol for high-throughput country-wide SARS-CoV-2 monitoringArticle Published on 2022-06-202023-07-08 Journal: The Science of the total environment [Category] COVID19(2023년), [키워드] COVID-19 wastewater-based surveillance PEG precipitation SARS-CoV-2 concentration Singapore Ultrafiltration [DOI] 10.1016/j.scitotenv.2022.154024 PMC 바로가기
Coronaviruses and SARS-CoV-2 in sewerage and their removal: Step by step in wastewater treatment plants하수도의 코로나바이러스 및 SARS-CoV-2 및 그 제거: 폐수 처리장의 단계별Review Published on 2022-05-012022-09-11 Journal: Environmental research [Category] COVID19(2023년), SARS, 치료기술, [키워드] activated adequate knowledge Adsorption Concentration condition Coronaviruses CoV CoVs debate decrease Detection limit digestion discharged Disinfection ENhance faece Faeces filtration help hydrophobic inactivated knowledge logarithm mesophilic microorganisms occur plant plants predation Prevent reduced required reuse SARS-CoV-2 sedimentation separated sewage significantly Sludge step temperature Transmission Treatment Ultrafiltration UV light Viral load viral loads virus wastewater Wastewater treatment plants [DOI] 10.1016/j.envres.2021.112204 PMC 바로가기 [Article Type] Review
Validating and optimizing the method for molecular detection and quantification of SARS-CoV-2 in wastewater폐수에서 SARS-CoV-2의 분자 검출 및 정량화 방법 검증 및 최적화Article Published on 2022-03-152022-09-11 Journal: The Science of the total environment [Category] MERS, SARS, 진단, 치료기술, [키워드] 229E best changes in Community community infections coronavirus COVID-19 COVID-19 disease cycle threshold disease DNA evaluated fecal FIVE followed by HCoV HCoV-229E hCoV-229E. human coronavirus Infection Isolation less methodology Mild molecular Neutral parameter PCR PMMoV prevalence of COVID-19 provide public health qPCR quantification reported RNA extraction RNA isolation kit RT-qPCR Salmon SARS-CoV-2 SARS-CoV-2 detection sensitivity significantly significantly increased Surveillance threshold Ultrafiltration Variation Viral Viral RNA virus viruses was used wastewater [DOI] 10.1016/j.scitotenv.2021.151434 PMC 바로가기 [Article Type] Article
A review on detection of SARS-CoV-2 RNA in wastewater in light of the current knowledge of treatment process for removal of viral fragments바이러스 단편 제거 처리 공정에 대한 최신 지식에 비추어 폐수 내 SARS-CoV-2 RNA 검출에 대한 검토Review Published on 2021-12-012022-09-11 Journal: Journal of environmental management [Category] SARS, 치료기술, [키워드] 2019-nCoV acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 affected Analysis Chain Reaction Community Concentration condition coronavirus Coronavirus 2019 COVID-19 COVID-19 pandemic dependent on discharge disease Disinfection efficient eliminating environmental factor environmental factors Epidemiology global pandemic globe glycol implication include knowledge membrane MOST National outbreak pathogen Pathogens Polyethylene glycol polymerase chain reaction quantification Quantitative quantitative reverse transcription-polymerase chain reaction reduce regions removal reported RNA route RT-qPCR SARS-CoV-2 SARS-CoV-2 RNA severe acute respiratory syndrome Coronavirus Spread Surface water Surveillance the SARS-CoV-2 to be done Transmission transmission of SARS-CoV-2 Transmission routes Treatment ultracentrifugation Ultrafiltration Untreated wastewater Viral concentration viral concentrations viral fragment virus Virus concentration method wastewater Wastewater treatment process Wastewater–based epidemiology (WBE). water water bodies WHO World Health Organisation Worry [DOI] 10.1016/j.jenvman.2021.113563 PMC 바로가기 [Article Type] Review
Development of a rapid viability RT-PCR (RV-RT-PCR) method to detect infectious SARS-CoV-2 from swabs면봉에서 감염성 SARS-CoV-2를 검출하는 신속한 생존력 RT-PCR(RV-RT-PCR) 방법 개발Article Published on 2021-11-012022-09-11 Journal: Journal of virological methods [Category] SARS, 치료기술, [키워드] 96-well plate acute respiratory syndrome coronavirus Algorithm Analysis antiviral drug COVID-19 COVID-19 pandemic Culture cycle threshold detect determine development enrichment epidemiological include increase in Incubation period Infectious SARS-CoV-2 Infectious virus PCR method Post-infection potential surface Rapid Rapid viability representing required respiratory resulting reverse transcriptase reverse transcriptase PCR RT-PCR SARS-CoV-2 SARS-CoV-2 RNA SARS-CoV-2 virion SARS-CoV-2 virions Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus-2 Spread Swab swab sample swab samples swabs Transmission Ultrafiltration viability Viral detection viral infection virions virus [DOI] 10.1016/j.jviromet.2021.114251 PMC 바로가기 [Article Type] Article
Capsid integrity RT-qPCR for the selective detection of intact SARS-CoV-2 in wastewater폐수에서 온전한 SARS-CoV-2의 선택적 검출을 위한 캡시드 무결성 RT-qPCRArticle Published on 2021-10-152022-09-11 Journal: The Science of the total environment [Category] SARS, 치료기술, 치료법, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus applicability Area biosafety level Biosafety Level 3 capsid Capsid integrity RT-qPCR collected Concentration Concentration method coronavirus determine determined by Effect EMA evaluated form Genome genomes glycol heat-inactivated IMPROVE intact virus Interpretation investigated level 3 MHV murine hepatitis virus PMA Polyethylene glycol positive positive result positive sample qPCR reagents RT-qPCR SARS-CoV-2 selective severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 surrogate tested Tokyo Ultrafiltration virus was used wastewater [DOI] 10.1016/j.scitotenv.2021.148342 PMC 바로가기 [Article Type] Article
An optimised protocol for detection of SARS-CoV-2 in stool대변에서 SARS-COV-2를 탐지하기위한 최적화 된 프로토콜Methodology Article Published on 2021-09-062022-09-01 Journal: BMC Microbiology [Category] SARS, 진단, 치료기술, 치료제, [키워드] buffer can be used CDC Chain Reaction Clinical practice Clinical-test complexity COVID-19 patients COVID19 detect detected in stool FMT Gut high variability implication inactivated individual limit of detection microbiota Nasopharyngeal swab Nasopharyngeal swab samples Particle persistence polymerase chain polymerase chain reaction Prevalence primer probes protocol provided Result RNA RNA extraction robust RT-qPCR SARS-CoV-2 Stool Stool sample stool samples supplementary material swab samples tested titre titres Transmission Ultrafiltration Viral viral particle Viral particles virus [DOI] 10.1186/s12866-021-02297-w PMC 바로가기 [Article Type] Methodology Article