viral transcript – Communicable Diseases Research

Structural investigations of proteins encoded by SARS-CoV-2

Article

Published on 2022-09-012022-11-16
Journal: FEBS Open Bio [Category] SARS, 변종, 유전자 메커니즘,

Theaflavin 3-gallate inhibits the main protease (M pro) of SARS-CoV-2 and reduces its count in vitro

Theaflavin 3-gallate는 SARS-CoV-2의 주요 프로테아제(M pro)를 억제하고 시험관 내에서 그 수를 감소시킵니다.

Article

Published on 2022-07-302022-09-11
Journal: Scientific Reports [Category] COVID19(2023년), SARS, 신약개발, 치료제,

Targeting genomic SARS-CoV-2 RNA with siRNAs allows efficient inhibition of viral replication and spread

siRNA로 게놈 SARS-CoV-2 RNA를 표적화하면 바이러스 복제 및 확산을 효율적으로 억제할 수 있습니다.

AcademicSubjects/SCI00010

Published on 2022-01-112022-09-10
Journal: Nucleic Acids Research [Category] 진단, 치료제,

[키워드] 3′ end active against acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus-2 antiviral efficacy approach cell entry conserved conserved region Coronavirus-2 COVID-19 discontinuous transcription effective Ex vivo Frame genomic genomic RNA gRNA human lung inhibited Open reading frame open reading frame 1 ORF1 Prevent Prophylactic Region replicate Replication respiratory RNA SARS-CoV-2 SARS-CoV-2 replication SARS-CoV-2 RNA sequence Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus sgRNA sgRNAs siRNA siRNAs Spread target targeting therapy transcript Transcription transcripts translational unique Verifying Viral viral replication Viral RNA viral transcript viral transcripts virus-induced cell death
[DOI] 10.1093/nar/gkab1248 PMC 바로가기 [Article Type] AcademicSubjects/SCI00010

Highly Efficient SARS-CoV-2 Infection of Human Cardiomyocytes: Spike Protein-Mediated Cell Fusion and Its Inhibition

인간 심근세포의 고효율 SARS-CoV-2 감염: 스파이크 단백질 매개 세포 융합 및 억제

Virus-Cell Interactions

Published on 2021-10-062022-09-10
Journal: Journal of Virology [Category] MERS, SARS, 변종,

[키워드] 2019 aberrant host response accounted ACE2 Activation activity Acute respiratory infection acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Amino acid angiotensin Angiotensin-converting enzyme angiotensin-converting enzyme 2 Autopsy block canonical cardiac cardiac complication Cardiac complications cardiac damage cardiac tropism cardiovascular complication cardiovascular complications catalytic site Cell Cell fusion cell lines cell surface cell-cell fusion cells cleavage cleavage site coding coronavirus coronavirus disease correlated COVID-19 COVID-19 patient cytopathic Cytopathic effect cytoplasm cytoplasmic tail direct infection disrupt Drug discovery efficient examined exceeded Express expressed expression vector fluorescent furin furin cleavage motif furin-like protease furin-like proteases fused fusing fusion fusion inhibition green fluorescent protein Human human cardiomyocyte in vitro model inactive Infection inhibition Lungs mechanism monomeric mRNA mutant Mutation Myocardium occur other coronavirus other coronaviruses parental Particle patients peptide-based platform pluripotent stem cell-derived cardiomyocytes protease Protein protein cleavage Proteolytic processing receptor replicate required respiratory respiratory infection SARS-CoV-2 SARS-COV-2 infection Serine serine 2 severe acute respiratory syndrome Coronavirus spike Spike protein Spread structures Syncytia systemic inflammation the spike protein These data thrombosis tissue TMPRSS2 Transformed cell transmembrane transmembrane protease serine 2 tropism of SARS-CoV-2 Vesicle Viral viral replication viral transcript viral transcription viral transcripts Virus-like particle virus-like particles viruses
[DOI] 10.1128/JVI.01368-21 PMC 바로가기 [Article Type] Virus-Cell Interactions

Single-nucleus transcriptome analysis of human brain immune response in patients with severe COVID-19

중증 COVID-19 환자의 인간 뇌 면역 반응에 대한 단일 핵 전사체 분석

Research

Published on 2021-07-192022-09-10
Journal: Genome Medicine [Category] SARS, 유전자 메커니즘, 치료기술,

[키워드] absence acute COVID-19 acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Analysis biological processes Brain brain region brain tissue caused cells cellular activation Choroid plexus Control coronavirus coronavirus disease Coronavirus disease 2019 cortical COVID-19 COVID-19 patients death detect detectable Differential gene expression droplet examined Gene Expression gene regulatory network Host immune response immune response individual cell individual cells individuals Infection Macrophage macrophages Medulla oblongata microglia Mobility Monocytes Neuroinflammation neurological Neuropsychiatric oblongata Patient phagocytosis Prefrontal cortex proportion Region Result RNA sequencing S1 protein SARS-CoV-2 SARS-CoV-2 genome serotypes severe acute respiratory syndrome Coronavirus severe COVID-19 Spike protein stromal cells supplementary material time of death trajectory Transcription transcriptional change Transcriptional changes Transcriptome transcriptomes Viral viral spike viral spike protein viral transcript viral transcriptome viral transcripts virus was performed western blot
[DOI] 10.1186/s13073-021-00933-8 PMC 바로가기 [Article Type] Research

Reverse-transcribed SARS-CoV-2 RNA can integrate into the genome of cultured human cells and can be expressed in patient-derived tissues

역전사된 SARS-CoV-2 RNA는 배양된 인간 세포의 게놈에 통합될 수 있으며 환자 유래 조직에서 발현될 수 있습니다.

422

Published on 2021-05-252022-09-10
Journal: Proceedings of the National Academy of Sciences of [Category] SARS, 유전자 메커니즘, 치료기술, 치료제,

[키워드] 3′ end absence acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 can not cellular chimeric chimeric RNAs clinical recovery Consensus contribute coronavirus COVID-19 Culture DNA duplication Evidence explain expressed fraction fusing Genome genomic integration help host cell human cell human cells Hypothesis in some infected cell Infection Infectious virus initial integration integration sites investigated LINE1 majority mechanism Patient patients PCR PCR-positive positive positive PCR test produced Prolonged recurrence reported respiratory reverse transcription RNA SARS-CoV-2 SARS-CoV-2 disease SARS-COV-2 infection SARS-CoV-2 infections SARS-CoV-2 RNA SARS-CoV-2 sequence SARS-CoV-2 sequences sequence severe acute respiratory syndrome Coronavirus Significance Support tissue tissues transcribed transcript Transcription transcripts Viral viral genome viral replication Viral RNA viral sequence viral sequences viral transcript viral transcripts
[DOI] 10.1073/pnas.2105968118 PMC 바로가기 [Article Type] 422

SARS-CoV-2 uses a multipronged strategy to impede host protein synthesis

Research

Published on 2021-05-122022-10-05
Journal: Nature [Category] SARS, 유전자 메커니즘,

Broad-Spectrum Antiviral Activity of 3D8, a Nucleic Acid-Hydrolyzing Single-Chain Variable Fragment (scFv), Targeting SARS-CoV-2 and Multiple Coronaviruses In Vitro

Article

Published on 2021-04-092022-10-28
Journal: Viruses [Category] COVID-19, MERS, SARS,

[키워드] 3D8 scFv activity acute respiratory syndrome antibody Antiviral antiviral activity Cell cell cultures cells coronavirus Coronaviruses COVID-19 Critical DNA and RNA epidemic diarrhea etiology evaluated exhibit fragment growth HCoV-OC43 human coronavirus OC43 in vitro inhibited MTT Multiple multiple coronaviruses novel coronavirus disease other coronavirus other coronaviruses pandemic Particles PEDV Plaque assay Prophylactic public health threat quantified Quantitative reduction replication of SARS-CoV-2 responsible RT-qPCR SARS-CoV-2 scFv single-chain variable fragment targeting therapeutic effect Vaccine variable Vero E6 VERO E6 cells viability viral transcript virus was determined zoonotic coronaviruses
[DOI] 10.3390/v13040650 PMC 바로가기 [Article Type] Article

SARS-CoV-2 Infects Human Pluripotent Stem Cell-Derived Cardiomyocytes, Impairing Electrical and Mechanical Function

Article

Published on 2021-02-132022-10-29
Journal: Stem Cell Reports [Category] COVID-19, MERS, SARS,

[키워드] ACE2 Alter arrhythmias and heart failure cardiac disease Cardiac infection cardiovascular complications cardiovascular disease cause caused cell death cellular chromatin contractile COVID-19 COVID-19 patient develop direct infection dose electrical Exocytosis fraction function functions Gene Expression hESC-CMs host gene expression hPSC-CMs Human human pluripotent stem cell-derived cardiomyocytes Hypothesis impair infect lysosome Mechanical metabolism Modification MOST mRNA oxidative Pathways receptor ACE2 replicate Replication RNA splicing SARS-CoV-2 SARS-COV-2 infection significantly smooth muscle cell STEM Support Symptom tropism of SARS-CoV-2 upregulate Vesicle viral entry viral infection viral myocarditis viral transcript widespread
[DOI] 10.1016/j.stemcr.2021.02.008 PMC 바로가기 [Article Type] Article

Coronavirus genomes carry the signatures of their habitats

코로나 바이러스 게놈은 서식지의 서명을 가지고 있습니다

Research Article

Published on 2020-12-222022-08-13
Journal: PLoS ONE [Category] MERS, SARS, 유전자 메커니즘, 진단,

[키워드] abundance Antiviral antiviral protein antiviral proteins Apolipoprotein B avoidance AVP bind Canis coronavirus CoV CpG Degradation dinucleotide effort enzyme evade Evolution Express facilitate generate Genome HIV-1 Homo Homo sapien Homo sapiens Host immune response host immune responses host immune system host tissue Hypothesis immune responses increase in infect infecting Infection Lupus MERS MHV motif mRNA Mus Mutation notable occur Protein residue SAME AS SARS-CoV SARS-CoV-2 SARS-CoV-2 genome SARS-CoV-2 vaccine selective pressure selective pressures sequence Seven Spread Temporal analysis tested the SARS-CoV-2 tissue tissues understanding variations Viral viral genome viral genomes viral transcript viral transcripts ZAP Zinc zinc finger antiviral protein
[DOI] 10.1371/journal.pone.0244025 PMC 바로가기 [Article Type] Research Article