The SARS-CoV-2 nucleoprotein associates with anionic lipid membranesResearch Article Published on 2024-06-102024-09-05 Journal: Brain stimulation [Category] update2024, [키워드] lipid-binding protein lipid-protein interaction Phosphatidylserine phosphoinositide RNA virus virus assembly [DOI] 10.1016/j.jbc.2024.107456 PMC 바로가기 [Article Type] Research Article
Live Cell Imaging Reveals HBV Capsid Translocation from the Nucleus To the Cytoplasm Enabled by Cell DivisionArticle Published on 2023-04-252023-06-13 Journal: mBio [Category] B형 간염, Fulltext, [키워드] core protein Hepatitis B virus live cell imaging nuclear export subcellular trafficking virus assembly [DOI] 10.1128/mbio.03303-22 PMC 바로가기
The “LLQY” Motif on SARS-CoV-2 Spike Protein Affects S Incorporation into Virus ParticlesSARS-CoV-2 스파이크 단백질의 "LLQY" 모티프는 S가 바이러스 입자에 통합되는 데 영향을 줍니다.Article Published on 2022-03-232022-09-11 Journal: Journal of Virology [Category] SARS, 변종, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus Affect Alter Amino acid amino acid residue amino acids antiviral drug antiviral drugs assembly biological significance Biosynthesis caused Cell culture cleavage cleavage site cleavage sites cleaved conformational conservation conserved Contact contribute coronavirus COVID-19 epidemic Critical demonstrated Effectiveness expressed glycoprotein hydrogen Hydrogen bond hydrogen bonds incorporation Infection intrachain junction key amino acid key amino acids less Lineage LLQY domain membrane fusion motif mutated Mutation Neutralizing antibodies Particle Prevent processing producer cell protease Protein protein synthesis protein synthesis and cleavage Pseudotyped virus pseudovirus pseudoviruses reduce residue S protein S1/S2 SARS-CoV-2 SARS-CoV-2 pseudoviruses SARS-CoV-2 variant SARS-CoV-2 variants sensitivity severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 spike Spike protein stability subcellular localization synthesis the S protein the SARS-CoV-2 Transmission tyrosine Vaccines viral entry viral infection virus virus assembly Virus-like particle virus-like particles [DOI] 10.1128/jvi.01897-21 PMC 바로가기 [Article Type] Article
Assembly and Entry of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV2): Evaluation Using Virus-Like Particles중증급성호흡기증후군 코로나바이러스 2(SARS-CoV2)의 조립 및 진입: 바이러스 유사 입자를 이용한 평가Article Published on 2021-04-092022-09-11 Journal: Cells [Category] MERS, SARS, 치료법, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus assembly Betacoronavirus BSL-3 can be used cell culture conditions chimeric complementary Complementation coronavirus coronavirus 2 COVID-19 Critical CTD cytoplasmic D614G dependent on domain effective envelope evaluate expressing Factors feature Features functional HiBiT homologous host cell inoculated investigated LgBiT matrix MERS-CoV MHV N protein N proteins NanoBiT technology nLuc nucleocapsid offer Particle peptide produced Proteins Release respiratory SARS-CoV2 secretion supported susceptibility target cell target cells Viral viral membrane virus virus assembly Virus-like particle virus-like particles VLP VLPs was used [DOI] 10.3390/cells10040853 PMC 바로가기 [Article Type] Article
Assembly and Cellular Exit of Coronaviruses: Hijacking an Unconventional Secretory Pathway from the Pre-Golgi Intermediate Compartment via the Golgi Ribbon to the Extracellular SpaceReview Published on 2021-02-262022-10-31 Journal: Cells [Category] COVID-19, MERS, [키워드] absence aggregate assembly bypasse carrier Cell compartment connection Coronavirus (CoV) CoV CoVs defined described element endocytic Endoplasmic reticulum endosome ER-Golgi intermediate compartment (IC or ERGIC) event exit extracellular space functional Golgi Golgi bypass Golgi ribbon GTPase indicate initial intermediate introduced Lipoproteins megavesicles pathway progeny promote Protein rab1 rab11 recycling endosome (RE) remained ribbon secretory Space Transport unconventional secretion vesicular tubular cluster (VTC) virus virus assembly virus egress viruses zone [DOI] 10.3390/cells10030503 PMC 바로가기 [Article Type] Review
An Intracellular Model of Hepatitis B Viral Infection: An In Silico Platform for Comparing Therapeutic StrategiesArticle Published on 2020-12-232023-06-02 Journal: Viruses [Category] B형 간염, [키워드] antiviral therapy Hepatitis B virus intracellular model packaging signal virus assembly [DOI] 10.3390/v13010011 PMC 바로가기 [Article Type] Article
The SARS-CoV-2 envelope and membrane proteins modulate maturation and retention of the spike protein, allowing assembly of virus-like particlesResearch Article Published on 2020-12-032022-10-30 Journal: The Journal of Biological Chemistry [Category] COVID-19, SARS, [키워드] acute respiratory syndrome biochemical C-terminal Cell composed coronavirus COVID-19 COVID-19 pandemic Critical cytoplasmic tail DMEM, Dulbecco’s modified minimal essential medium E, Envelope Endoplasmic reticulum ERGIC ERGIC, endoplasmic reticulum (ER)–Golgi intermediate compartment glycoprotein Golgi highlight IBV, infectious bronchitis virus IF, immunofluorescence induce infected cell Infectious disease Interaction Like M protein M, membrane maturation membrane membrane protein MERS-CoV, Middle East Respiratory Virus modulate modulating motif N-glycosylation N, nucleoprotein other coronaviruses Particle Particles Prevent promote Protein Proteins purified regulate Regulation required S protein S, spike SARS-CoV SARS-CoV-2 SARS-CoV-2 envelope SARS-CoV-2 viral particles SARS-CoV-2-infected cells SARS-CoV-2, Severe acute respiratory syndrome coronavirus 2 secretion secretory pathway structural protein syncytia formation the spike protein transfected cells Viral particles Viral protein virus assembly Virus-like particle VLPs, Virus-like particles vRNP, viral ribonucleoprotein β-coronavirus [DOI] 10.1074/jbc.RA120.016175 PMC 바로가기 [Article Type] Research Article
SARS-CoV-2 viral budding and entry can be modeled using BSL-2 level virus-like particlesResearch Article Published on 2020-11-272022-10-30 Journal: The Journal of Biological Chemistry [Category] COVID-19, SARS, [키워드] ACE2, angiotensin-converting enzyme 2 acute respiratory syndrome BCA, bicinchoninic acid assay BSL-2 BSL-3 BSL-3 facilities circumvent competent conditions coronavirus DAB, 3,3′-Diaminobenzidine DMEM, Dulbecco’s modified Eagle’s medium EEA1, early endosome antigen 1 effort electron microscopy (EM) ERGIC, endoplasmic reticulum–Golgi intermediary complex FBS, fetal bovine serum genetic material global pandemic globe Golgi human cell human cells inaccessible inhibitor Laboratory LAMP1, lysosomal associated membrane protein 1 majority mechanism membrane bilayer MERS coronaviruses MERS-CoV, Middle East respiratory syndrome coronavirus ORFs, open reading frames pandemic PFA, paraformaldehyde producing Research resource SARS-CoV-1 SARS-CoV-2 SARS-CoV-2 research SARS-CoV-2 viral SARS-CoV-2 VLP SARS-CoV-2, Severe acute respiratory syndrome coronavirus 2 SEM, scanning electron microscopy Spread structural protein TEM, transmission electron microscopy viral life cycle Viral protein Virology virus virus assembly virus budding virus entry Virus-like particle virus-like particle (VLP) VLPs VLPs, Virus-like particles WGA, wheat germ agglutinin Wuhan, China [DOI] 10.1074/jbc.RA120.016148 PMC 바로가기 [Article Type] Research Article
Molecular Architecture of the SARS-CoV-2 VirusArticle Published on 2020-10-292023-06-18 Journal: Cell [Category] Fulltext, SARS, [키워드] coronavirus cryo-electron tomography cryo-EM ribonucleoprotein SARS-CoV-2 spike glycoprotein subtomogram averaging virus assembly [DOI] 10.1016/j.cell.2020.09.018 PMC 바로가기
Host Cell Rab GTPases in Hepatitis B Virus InfectionReview Published on 2018-11-192023-08-30 Journal: Frontiers in Cell and Developmental Biology [Category] E형 간염, [키워드] autophagy HBV Rab effector Rab GAP Rab33B RAB7A virus assembly virus trafficking [DOI] 10.3389/fcell.2018.00154 PMC 바로가기 [Article Type] Review