Evaluation of low-cost SARS-CoV-2 RNA purification methods for viral quantification by RT-qPCR and next-generation sequencing analysis: Implications for wider wastewater-based epidemiology adoptionResearch Article Published on 2023-05-162024-09-05 Journal: Heliyon [Category] update2024, [키워드] COVID-19 RNA purification SARS-CoV-2 Viral genomic surveillance Virus quantification Wastewater-based epidemiology [DOI] 10.1016/j.heliyon.2023.e16130 PMC 바로가기 [Article Type] Research Article
Hepatitis E Virus: Isolation, Propagation, and QuantificationArticle Published on 2023-01-012023-06-11 Journal: Current protocols [Category] E형 간염, [키워드] HEV Isolation Propagation Virus quantification [DOI] 10.1002/cpz1.642
SARS-CoV-2 Viral Load in the Pulmonary Compartment of Critically Ill COVID-19 Patients Correlates with Viral Serum Load and Fatal Outcomes중환자 COVID-19 환자의 폐실에서 SARS-CoV-2 바이러스 부하가 바이러스 혈청 부하 및 치명적인 결과와 상관관계Article Published on 2022-06-142022-09-11 Journal: Viruses [Category] COVID19(2023년), SARS, 치료기술, [키워드] association BAL Blood bronchoalveolar Bronchoalveolar lavage clinical Clinical data Clinical outcome clinical outcomes compartment contribute copy number correlation COVID-19 COVID-19 disease COVID-19 disease severity Critical Critical care Critically ill Critically ill patient critically ill patients Croatia diagnostic disease elevated highest hospital ICU admission Ill intensive care intensive care unit load Lower respiratory tract lung Lungs mechanical ventilation support outcome outcomes pandemic parameter Predictive Prognostic value pulmonary qPCR quantification quantified SARS-CoV-2 SARS-CoV-2 detection serum sample serum samples sputum survival Swab swabs upper respiratory tract Viral load viral load analysis viral loads Virus quantification virus titers while [DOI] 10.3390/v14061292 PMC 바로가기 [Article Type] Article
Digital PCR can augment the interpretation of RT-qPCR Cq values for SARS-CoV-2 diagnostics디지털 PCR은 SARS-CoV-2 진단을 위한 RT-qPCR Cq 값의 해석을 보강할 수 있습니다.Article Published on 2022-05-012022-09-11 Journal: Methods (San Diego, Calif.) [Category] COVID19(2023년), MERS, SARS, 유전자 메커니즘, 진단, [키워드] acute health crisis acute respiratory disease Analysis bile salt bile salts caused common knowledge Compound coronavirus coronavirus disease Coronavirus disease 2019 coronavirus SARS-CoV-2 COVID-19 Cq value Cq value validity Cq values cut-off value cycle threshold demonstrated diagnostics Digital digital PCR disagreement disease dPCR Effect Efficiency effort exacerbated Health Health crisis highlight in urine include individual individuals infectious inhibiting inhibitor inhibitors Interpretation investigated knowledge MIQE and dMIQE guidelines molecular parameters PCR performed positive result qPCR quantification quantitative PCR Reporting Respiratory disease reverse transcription Reverse transcription quantitative PCR RNA extraction RT-qPCR Saliva Sample Sample matrix effects SARS-CoV-2 separated shown Standard Standard material susceptible Swab test results the disease These data Transmission Urine Validity Variability variable Viral viral burden Viral RNA Virus quantification [DOI] 10.1016/j.ymeth.2021.08.006 PMC 바로가기 [Article Type] Article
SARS-CoV-2 RNA detected in urban wastewater from Porto, Portugal: Method optimization and continuous 25-week monitoring포르투갈 포르토의 도시 폐수에서 SARS-CoV-2 RNA 검출: 분석법 최적화 및 25주 연속 모니터링Article Published on 2021-10-202022-09-11 Journal: The Science of the total environment [Category] SARS, 치료기술, [키워드] Analysis analyzed can be used centrifugation circulation city clinical testing collected complement Concentration continuous monitoring COVID-19 COVID-19 incidence COVID-19 pandemic develop effort eight Epidemiology evaluate first stage fraction implementation Improvements iterative Measures methodological methodology optimization organized pandemic plant Porto Portugal protocol public health quantification quantitative PCR reverse transcription Reverse transcription quantitative PCR RNA RNA extraction RT-qPCR SARS-CoV-2 SARS-CoV-2 RNA SARS-CoV-2 RNA detection selected Specific Support Surveillance the SARS-CoV-2 treated Treatment virus Virus quantification wastewater Wastewater-based epidemiology [DOI] 10.1016/j.scitotenv.2021.148467 PMC 바로가기 [Article Type] Article
Application of One-Step Reverse Transcription Droplet Digital PCR for Dengue Virus Detection and Quantification in Clinical SpecimensArticle Published on 2021-04-012023-08-30 Journal: Diagnostics [Category] E형 간염, [키워드] dengue virus droplet digital PCR virus detection Virus quantification [DOI] 10.3390/diagnostics11040639 PMC 바로가기 [Article Type] Article
Seasonal and diurnal surveillance of treated and untreated wastewater for human enteric virusesResearch Article Published on 2018-09-272023-08-30 Journal: Environmental science and pollution research inter [Category] E형 간염, [키워드] Activated sludge Autosampler Biofilter Sampling method Sewage treatment Virus quantification Water pollution [DOI] 10.1007/s11356-018-3261-y PMC 바로가기 [Article Type] Research Article
Hepatitis E Virus: Isolation, Propagation, and QuantificationArticle Published on 2018-02-222023-06-11 Journal: Curr Protoc Microbiol [Category] E형 간염, [키워드] HEV Isolation Propagation Virus quantification [DOI] 10.1002/cpmc.50
Bovine coronavirus in naturally and experimentally exposed calves; viral shedding and the potential for transmissionResearch Published on 2016-06-132022-10-28 Journal: Virology Journal [Category] Coronavirus, [키워드] analyzed antibodies BCoV BCV biosecurity Blood Clinical signs concerning conducted Contact coronavirus correlated cough Depression detectable disease euthanized experiment Experimental infection fecal group Hypothesis Infection information investigated knowledge lack lymphatic tissue Mild naïve nasal nasal swabs occur pathogen potential for transmission prevention producing Prolonged provide rectal respiratory rate Respiratory system Result RNA RT-qPCR secretion seropositive supplementary material swabs temperature tissue tissue sample transmission potential Viral RNA viral shedding Viremia virus virus isolation Virus quantification Virus shedding virus spread was performed [DOI] 10.1186/s12985-016-0555-x PMC 바로가기 [Article Type] Research