Virus titration – Communicable Diseases Research

Infection and Transmission of SARS-CoV-2 B.1.617.2 Lineage (Delta Variant) among Fully Vaccinated Individuals

Article

Published on 2022-10-262022-11-15
Journal: Microbiology Spectrum [Category] COVID19(2023년), SARS, 변종, 치료기술,

Optimization and evaluation of a live virus SARS-CoV-2 neutralization assay

살아있는 바이러스 SARS-CoV-2 중화 분석의 최적화 및 평가

Article

Published on 2022-07-282022-09-11
Journal: PLoS ONE [Category] COVID19(2023년), MERS, SARS, 변종, 진단, 치료법,

[키워드] 24 hour 24 hours acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 added antibody application calculated cell monolayer cell suspension cells clinical isolates Coefficient of variance coronavirus correlated correlation Critical described dose ELISA enzyme enzyme-linked immunosorbent enzyme-linked immunosorbent assay evaluated functional antibody response functional antibody responses growth immune evasion Immunity include Incubation period infecting Influenza Influenza virus inoculation isolates Live virus microneutralization neutralization Neutralization assay neutralization titers nucleocapsid protein optimization parameter Post-infection Precision protein level reduced replicating virus respiratory SARS-CoV-2 SARS-CoV-2 clinical isolate SARS-CoV-2 clinical isolates SARS-CoV-2 microneutralization assay SARS-CoV-2 neutralization severe acute respiratory syndrome Coronavirus the SARS-CoV-2 the strongest therapeutic Vaccine variance Vero E6 Vero E6 cell VERO E6 cells Viral Viral variants virus virus infection virus inhibition virus inoculum virus neutralization assays Virus titration was determined WHO
[DOI] 10.1371/journal.pone.0272298 PMC 바로가기 [Article Type] Article

Improving human coronavirus OC43 (HCoV-OC43) research comparability in studies using HCoV-OC43 as a surrogate for SARS-CoV-2

Article

Published on 2021-10-092022-10-04
Journal: Journal of virological methods [Category] MERS, SARS, 치료기술, 치료제,

[키워드] accompany acute respiratory syndrome added African green monkey analyzed antibodies antibody concentration approach biosafety BSL-3 Cell cell line cells Colon cancer Combination common cold comparability complicate conducted coronavirus counter CPE Culture Cytopathic effect days post infection demonstrated described droplet fibroblast Flow virometry growth Growth Curve HCoV-OC43 human coronavirus human coronavirus OC43 human lung Improving Infection infectious dose investigated kidney less multiplicity of infection pandemic parameter Plaque assay produced quantification quantified reached reducing reproducibility required Research respiratory aerosol Respiratory system Sample SARS-CoV-2 Sartorius Seven Standardization target TCID these cell tissue transmitted Vero E6 Vero E6 cell virion virus particle Virus titration
[DOI] 10.1016/j.jviromet.2021.114317 PMC 바로가기 [Article Type] Article

Discrepancies in Infectivity of Flavivirus and SARS-CoV-2 Clinical Samples: An Improved Assay for Infectious Virus Shedding and Viremia Assessment

플라비바이러스와 SARS-CoV-2 임상 샘플의 감염력 불일치: 감염성 바이러스 배출 및 바이러스혈증 평가를 위한 개선된 분석

Article

Published on 2021-09-182022-09-10
Journal: International Journal of Environmental Research an [Category] SARS, 치료기술,

[키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addition alternative Antibody titers assessment Avicel Cellulose clinical clinical sample clinical samples coronavirus COVID-19 culture medium Dengue evaluated flavivirus Improved infectious particles Infectivity infectivity assessment Laboratory MCC medium methylcellulose microcrystalline microcrystalline cellulose Neutralizing antibody titer neutralizing antibody titers nucleic acid nucleic acid amplification nucleic acid amplification tests overlay medium Particle plaque Plaque assay plaques respiratory SARS-CoV-2 SARS-CoV-2 strains sensitivity severe acute respiratory syndrome Coronavirus shedding significantly time-consuming Viremia virus virus infectivity virus strains virus titer virus titers Virus titration was performed was used while
[DOI] 10.3390/ijerph18189845 PMC 바로가기 [Article Type] Article

In vitro Characterization of Fitness and Convalescent Antibody Neutralization of SARS-CoV-2 Cluster 5 Variant Emerging in Mink at Danish Farms

Microbiology

Published on 2021-06-252022-10-31
Journal: Frontiers in Microbiology [Category] COVID-19,

[키워드] Active surveillance acute respiratory syndrome addition alteration Amino acid amino acid change amino acid residue amino acid substitution animal hosts antigenicity cell line characterization characterized consequence convalescent coronavirus country COVID-19 COVID-19 samples cynomolgus macaques Deletion demonstrated Denmark Europe evaluated Evolution farm ferrets fitness genomic hamsters humans in vitro include microneutralization mink neutralization North America Potential proportion quantitation reduced reported rhesus macaques SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 variant Spread Substitution susceptible Temporal the spike protein variant VERO-E6 Viral RNA virulence virus Virus neutralization Virus titration Wuhan-Hu-1 Y453F
[DOI] 10.3389/fmicb.2021.698944 PMC 바로가기 [Article Type] Microbiology

An in vitro model for assessment of SARS-CoV-2 infectivity by defining the correlation between virus isolation and quantitative PCR value: isolation success of SARS-CoV-2 from oropharyngeal swabs correlates negatively with Cq value

바이러스 분리와 정량적 PCR 값 간의 상관 관계를 정의하여 SARS-CoV-2 감염성을 평가하기 위한 시험관 내 모델: 구강 인두 면봉에서 SARS-CoV-2의 분리 성공은 Cq 값과 음의 상관 관계가 있습니다.

Research

Published on 2021-04-072022-09-01
Journal: Virology Journal [Category] MERS, SARS, 진단,

[키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Applying calculated caused Cell Cell culture Chain Reaction coronavirus correlation COVID-19 patient Cq value decrease Diagnosis E-gene Epidemiological study experiment high risk highest in vitro in vitro model indication induce Infection infectious infective Infectivity inoculated Isolation Laboratory testing less long-lasting negative correlation novel Oropharyngeal oropharyngeal swab pandemic Pathogens Patient PCR polymerase chain polymerase chain reaction positive Probability Public public health qPCR Quantitation of viral infectivity Quantitative quantitative PCR quantitative polymerase chain reaction question raise respiratory Result retrospective risk factor risk of transmission RNA SARS-CoV-2 SARS-CoV2 severe acute respiratory syndrome Coronavirus significantly symptomatic Transmissibility Transmission Transmission pattern in vitro Viral Viral load viral pathogens virus virus isolation Virus titration
[DOI] 10.1186/s12985-021-01542-y PMC 바로가기 [Article Type] Research

Biopolymeric nano/microspheres for selective and reversible adsorption of coronaviruses

Article

Published on 2017-03-062023-06-15
Journal: Materials Science & Engineering. C, Materials for Biological Applications [Category] MERS,

[키워드] Adsorbent Adsorption chitosan coronavirus Cytopathic effect Genipin Microspheres Nanospheres polymerase chain reaction Virus titration
[DOI] 10.1016/j.msec.2017.03.047 PMC 바로가기 [Article Type] Article