‘Long Covid’, or medical complications associated with post SARS-CoV-2 infection, is a significant post-viral complication that is being more and more commonly reported in patients. Therefore, there is an increasing need to understand the disease mechanisms, identify drug targets and inflammatory processes associated with a SARS-CoV-2 infection. To address this need, we created a targeted mass spectrometry based multiplexed panel of 96 immune response associated proteins. We applied the multiplex assay to a cohort of serum samples from asymptomatic and moderately affected patients. All patients had tested positive for a SARS-CoV-2 infection by PCR and were determined to be subsequently positive for antibodies. Even 40-60 days post-viral infection, we observed a significant remaining inflammatory response in all patients. Proteins that were still affected were associated with the anti-inflammatory response and mitochondrial stress. This indicates that biochemical and inflammatory pathways within the body can remain perturbed long after SARS-CoV-2 infections have subsided even in asymptomatic and moderately affected patients.
【저자키워드】 SARS-CoV-2, Inflammation, proteomics, mass spectrometry, Biomarker, 【초록키워드】 antibodies, viral infection, mass spectrometry, immune response, Stress, SARS-COV-2 infection, Infection, Proteins, Cohort, PCR, Multiplex assay, Asymptomatic, Patient, Complication, drug target, mechanisms, SARS-CoV-2 infections, patients, Inflammatory response, Inflammatory, mitochondrial, biochemical, serum samples, anti-inflammatory response, positive, serum sample, tested, identify, affected, reported, the disease, applied, indicate, inflammatory pathway, 【제목키워드】 Patient, Inflammatory response, affected,