Identification of patients with activation of the immune system which indicates the presence of infection is essential, especially in the times of the global coronavirus 2019 (COVID-19) pandemic. The aim of the present study was to evaluate the reactive lymphocytes (RE-LYMP) parameter in COVID-19 and to correlate it with activation lymphocytes markers by flow cytometry. The study group consisted of 40 patients: with COVID-19 infection ( n = 20) and with others virus infections without COVID-19 (COVID-19(−) virus ( n = 20)) and 20 healthy donors (HC). Blood count and flow cytometry were performed. The COVID-19(+) group had significantly lower RE-LYMP parameter than the COVID-19(−) virus group (5.45 vs. 11.05, p < 0.05). We observed higher proportion of plasmablasts in the COVID-19(+) and COVID-19(−) virus groups than HC (8.8 vs. 11.1 vs. 2.7, p < 0.05). In the COVID-19(+) there was a lower proportion of CD4+ CD38+ cells than in the other groups (significant differences between COVID-19(+) and COVID-19(−) virus groups). RE-LYMP correlated with activated T lymphocytes CD38+ and HLA-DR+ in the COVID-19(−) virus group, however in the COVID-19(+) group correlations with T lymphocytes CD25+ and CD45RO+ were observed. In summary the analysis of the RE-LYMP together with flow cytometric activation markers can be helpful in identifying and distinguishing patients with COVID-19(+) from other viruses and HC.
【저자키워드】 COVID-19, flow cytometry, plasmablasts, reactive lymphocytes, CD25, CD45RO, HLA-DR, CD38, 【초록키워드】 viruses, coronavirus, pandemic, Lymphocytes, Infection, immune system, virus, flow cytometry, lymphocyte, COVID-19 infection, plasmablasts, Patient, T lymphocytes, virus infection, Coronavirus 2019, correlation, group, CD25, CD45RO, CD38, virus infections, marker, blood count, Analysis, identification, the times, Activation, study group, significant differences, T lymphocyte, CD4+, plasmablast, significantly lower, parameter, Cell, reactive, flow cytometric, healthy donor, performed, evaluate, proportion, activated, indicate, correlated, groups, CD38+, with COVID-19, 【제목키워드】 Lymphocytes, Flow, New,