Coronavirus disease 2019 (COVID-19), a global pandemic, is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Angiotensin-converting enzyme 2 (ACE2) is the receptor for SARS-CoV-2 and transmembrane serine protease 2 (TMPRSS2) facilitates ACE2-mediated virus entry. Moreover, the expression of ACE2 in the testes of infertile men is higher than normal, which indicates that infertile men may be susceptible to be infected and SARS-CoV-2 may cause reproductive disorder through the pathway induced by ACE2 and TMPRSS2 . Little is known about the pathway regulation of ACE2 and TMPRSS2 expression in male reproductive disorder. Since the regulation of gene expression is mediated by microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) at the post-transcriptional level, the aim of this study was to analyze the dysregulated miRNA–lncRNA interactions of ACE2 and TMPRSS2 in male reproductive disorder. Using bioinformatics analysis, we speculate that the predicted miRNAs including miR-125a-5p , miR-125b-5p , miR-574-5p , and miR-936 as regulators of ACE2 and miR-204-5p as a modulator of TMPRSS2 are associated with male infertility. The lncRNAs with a tissue-specific expression for testis including GRM7-AS3, ARHGAP26-AS1, BSN-AS1, KRBOX1-AS1, CACNA1C-IT3, AC012361.1, FGF14-IT1, AC012494.1, and GS1-24F4.2 were predicted. The identified miRNAs and lncRNAs are proposed as potential biomarkers to study the possible association between COVID-19 and male infertility. This study encourages further studies of miRNA–lncRNA interactions to explain the molecular mechanisms of male infertility in COVID-19 patients.
【저자키워드】 COVID-19, miRNA, male infertility, lncRNA, interactions, TMPRSS2, 【초록키워드】 coronavirus disease, SARS-CoV-2, Coronavirus disease 2019, ACE2, coronavirus, Gene Expression, microRNA, molecular mechanism, severe acute respiratory syndrome Coronavirus, angiotensin-converting enzyme 2, global pandemic, male infertility, miRNAs, male, virus entry, pathway, receptor, respiratory, transmembrane serine protease 2, Bioinformatics analysis, expression, MicroRNAs, long non-coding RNA, COVID-19 patients, infertility, Testis, association, Angiotensin-converting enzyme, Potential biomarker, Interaction, angiotensin, acute respiratory syndrome, Regulation, Serine, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, enzyme, molecular mechanisms, transmembrane serine protease, TMPRSS2 expression, potential biomarkers, testes, modulator, disorder, post-transcriptional level, tissue-specific expression, ARHGAP26, ARHGAP26-AS1, BSN-AS1, CACNA1C, CACNA1C-IT3, FGF14, FGF14-IT1, GRM7, GRM7-AS3, GS1-24F4.2, KRBOX1, KRBOX1-AS1, men, susceptible, predicted, caused, facilitate, indicate, dysregulated, explain, reproductive, miR-125a-5p, 【제목키워드】 identification, with COVID-19,