The global outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is still ongoing, as is research on the molecular mechanisms underlying cellular infection by coronaviruses, with the hope of developing therapeutic agents against this pandemic. Other important respiratory viruses such as 2009 pandemic H1N1 and H7N9 avian influenza virus (AIV), influenza A viruses, are also responsible for a possible outbreak due to their respiratory susceptibility. However, the interaction of these viruses with host cells and the regulation of post-transcriptional genes remains unclear. In this study, we detected and analyzed the comparative transcriptome profiling of SARS-CoV-2, panH1N1 (A/California/07/2009), and H7N9 (A/Shanghai/1/2013) infected cells. The results showed that the commonly upregulated genes among the three groups were mainly involved in autophagy, pertussis, and tuberculosis, which indicated that autophagy plays an important role in viral pathogenicity. There are three groups of commonly downregulated genes involved in metabolic pathways. Notably, unlike panH1N1 and H7N9, SARS-CoV-2 infection can inhibit the m-TOR pathway and activate the p53 signaling pathway, which may be responsible for unique autophagy induction and cell apoptosis. Particularly, upregulated expression of IRF1 was found in SARS-CoV-2, panH1N1, and H7N9 infection. Further analysis showed SARS-CoV-2, panH1N1, and H7N9 infection-induced upregulation of lncRNA-34087.27 could serve as a competitive endogenous RNA to stabilize IRF1 mRNA by competitively binding with miR-302b-3p. This study provides new insights into the molecular mechanisms of influenza A virus and SARS-CoV-2 infection.
【저자키워드】 SARS-CoV-2, influenza A, autophagy, noncoding RNA, IRF1, 【초록키워드】 viruses, Transcriptome, Apoptosis, Coronaviruses, coronavirus, pandemic, Tuberculosis, Influenza, SARS-COV-2 infection, susceptibility, Infection, influenza A virus, Influenza virus, molecular mechanism, severe acute respiratory syndrome Coronavirus, virus, influenza A, autophagy, RNA, Viral, respiratory virus, outbreak, mRNA, Research, pathway, lncRNA, therapeutic agent, H1N1, therapeutic agents, signaling pathway, respiratory, pathogenicity, Other, expression, IRF1, binding, cellular, Interaction, p53, Analysis, metabolic pathways, Hope, pertussis, host cells, host cell, Avian Influenza, H7N9, acute respiratory syndrome, Regulation, these viruses, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, infected cells, molecular mechanisms, three groups, upregulation, viral pathogenicity, upregulated gene, influenza A viruses, IRF1 mRNA, p53 signaling pathway, Cell, responsible, analyzed, involved, indicated, inhibit, provide, in viral, unique, upregulated, activate, three group, downregulated gene, 【제목키워드】 RNA, response, network, endogenous, SARS-CoV-2-,