To determine whether the neutralization activity of monoclonal antibodies, convalescent sera and vaccine-elicited sera was affected by the top five epidemic SARS-CoV-2 variants in the UK, including D614G+L18F+A222V, D614G+A222V, D614G+S477N, VOC-202012/01(B.1.1.7) and D614G+69-70del+N439K, a pseudovirus-neutralization assay was performed to evaluate the relative neutralization titers against the five SARS-CoV-2 variants and 12 single deconvolution mutants based on the variants. In this study, 18 monoclonal antibodies, 10 sera from convalescent COVID-19 patients, 10 inactivated-virus vaccine-elicited sera, 14 mRNA vaccine-elicited sera, nine RBD-immunized mouse sera, four RBD-immunized horse sera, and four spike-encoding DNA-immunized guinea pig sera were tested and analyzed. The N501Y, N439K, and S477N mutations caused immune escape from nine of 18 mAbs. However, the convalescent sera, inactivated virus vaccine-elicited sera, mRNA vaccine-elicited sera, spike DNA-elicited sera, and recombinant RBD protein-elicited sera could still neutralize these variants (within three-fold changes compared to the reference D614G variant). The neutralizing antibody responses to different types of vaccines were different, whereby the response to inactivated-virus vaccine was similar to the convalescent sera.
【저자키워드】 Vaccine, Mutation, neutralization, monoclonal antibody, Pseudotyped virus, B.1.1.7, 【초록키워드】 mRNA vaccine, variant, SARS-CoV-2 variant, monoclonal antibodies, virus, variants, Epidemic, Immune escape, SARS-CoV-2 variants, B.1.1.7, RBD, N501Y, mRNA, D614G variant, sera, mutant, change, N439K, convalescent sera, Neutralizing antibody response, mAbs, inactivated, convalescent COVID-19 patients, guinea pig, neutralization activity, neutralizing antibody responses, neutralization titer, S477N, recombinant RBD protein, FIVE, neutralize, tested, analyzed, affected, evaluate, caused, nine, was performed, determine, 【제목키워드】 SARS-CoV-2, United Kingdom,